Thomas Damgaard Sandahl

Mass spectrometry characterization of circulating human serum albumin microheterogeneity in patients with alcoholic hepatitis

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Marina Naldi, Department of Pharmacy and Biotechnology, Via Sand Donato 15, 40127, University of Bologna, Italy; Center for Applied Biomedical Research (C.R.B.A.), S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy.
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  • Maurizio Baldassarre, Center for Applied Biomedical Research (C.R.B.A.), S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy; Department of Medical and Surgical Sciences, Via Massarenti 9, 40138, University of Bologna, Italy.
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  • Marco Domenicali, Center for Applied Biomedical Research (C.R.B.A.), S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy; Department of Medical and Surgical Sciences, Via Massarenti 9, 40138, University of Bologna, Italy.
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  • Ferdinando Antonino Giannone, Center for Applied Biomedical Research (C.R.B.A.), S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy; Department of Medical and Surgical Sciences, Via Massarenti 9, 40138, University of Bologna, Italy.
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  • Matteo Bossi, Department of Medical and Surgical Sciences, Via Massarenti 9, 40138, University of Bologna, Italy.
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  • Jonathan Montomoli, Institut for Klinisk Medicin - Medicinsk afdeling V, NBG
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  • Thomas Damgaard Sandahl
  • Emilie Glavind
  • Hendrik Vilstrup
  • Paolo Caraceni, Center for Applied Biomedical Research (C.R.B.A.), S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy; Department of Medical and Surgical Sciences, Via Massarenti 9, 40138, University of Bologna, Italy.
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  • Carlo Bertucci, Department of Pharmacy and Biotechnology, Via Sand Donato 15, 40127, University of Bologna, Italy. Electronic address: carlo.bertucci@unibo.it.

Human serum albumin (HSA) is the most abundant plasma protein, endowed with several biological properties unrelated to its oncotic power, such as antioxidant and free-radicals scavenging activities, binding and transport of many endogenous and exogenous substances, and regulation of endothelial function and inflammatory response. These non-oncotic activities are closely connected to the peculiarly dynamic structure of the albumin molecule. HSA undergoes spontaneous structural modifications, mainly by reaction with oxidants and saccharides; however, patients with cirrhosis show extensive post-transcriptional changes at several molecular sites of HSA, the degree of which parallels the severity of the disease. The present work reports the development and application of an innovative LC-MS analytical method for a rapid and reproducible determination of the relative abundance of HSA isoforms in plasma samples from alcoholic hepatitis (AH) patients. A condition of severe oxidative stress, similar to that observed in AH patients, is associated with profound changes in circulating HSA microheterogeneity. More interestingly, the high resolution provided by the analytical platform allowed the monitoring of novel oxidative products of HSA never reported before.

Original languageEnglish
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume122
Pages (from-to)141-7
Number of pages7
ISSN0731-7085
DOIs
Publication statusPublished - 15 Apr 2016

    Research areas

  • Journal Article, Research Support, Non-U.S. Gov't

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