Thomas Damgaard Sandahl

Changes in the levels of mannan-binding lectin and ficolins during head-down tilted bed rest

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Changes in the levels of mannan-binding lectin and ficolins during head-down tilted bed rest. / Kelsen, Jens; Sandahl, Thomas D; Storm, Line et al.

In: Aviation, Space, and Environmental Medicine, Vol. 85, No. 8, 08.2014, p. 805-11.

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Kelsen, Jens ; Sandahl, Thomas D ; Storm, Line et al. / Changes in the levels of mannan-binding lectin and ficolins during head-down tilted bed rest. In: Aviation, Space, and Environmental Medicine. 2014 ; Vol. 85, No. 8. pp. 805-11.

Bibtex

@article{9023f48284924954b90397eee2e03027,
title = "Changes in the levels of mannan-binding lectin and ficolins during head-down tilted bed rest",
abstract = "BACKGROUND: Spaceflight studies and ground-based analogues of microgravity indicate a weakening of human immunity. Mannan-binding lectin (MBL) and H-, L-, and M-ficolin together constitute the lectin pathway and mediate the clearance of pathogens through complement activation. We hypothesized that simulated microgravity may weaken human innate immune functions and studied the impact of 6° head-down tilted bed rest (HDT) for 21 d on MBL and ficolin levels.METHODS: Within a 6-mo period, seven men underwent two periods of HDT. Blood samples were analyzed for MBL, H-, L-, and M-ficolin, mannose-binding lectin-associated protein of 44 kDa (MAp44), and collectin liver 1 (CL-L1) by time-resolved immunofluorometric assays (TRIFMA).RESULTS: We observed well-defined individual preintervention levels of MBL and ficolins. Remarkably similar intraindividual changes occurred for MBL and MBL levels decreased (mean 282 ng · ml(-1)) in the recovery phase. Conversely, CL-L1, a protein with MBL-like properties, increased (mean 102 ng · ml(-1)) during the recovery phase. M-ficolin increased (mean 79 ng · ml(-1)) within the first 2 d of HDT, followed by a decrease (mean 112 ng · ml(-1)) during the recovery phase. L-ficolin increased (mean 304 ng · ml(-1)) during HDT, while H-ficolin was essentially unaffected. MAp44, a down-regulator of the lectin pathway, decreased initially (mean 78 ng · ml(-1)) in the recovery phase followed by an increase (mean 131 ng · ml(-1)).DISCUSSION: Alterations in MBL and ficolin levels were modest and with our current knowledge do not lead to overt immunodeficiency. Pronounced changes occurred when the subjects resumed the upright position. In selected individuals, these changes appear to be a conserved response to HDT.",
author = "Jens Kelsen and Sandahl, {Thomas D} and Line Storm and Petra Frings-Meuthen and Dahlerup, {Jens F} and Steffen Thiel",
year = "2014",
month = aug,
doi = "10.3357/ASEM.3915.2014",
language = "English",
volume = "85",
pages = "805--11",
journal = "Aviation, Space, and Environmental Medicine",
issn = "0095-6562",
publisher = "Aerospace Medical Association",
number = "8",

}

RIS

TY - JOUR

T1 - Changes in the levels of mannan-binding lectin and ficolins during head-down tilted bed rest

AU - Kelsen, Jens

AU - Sandahl, Thomas D

AU - Storm, Line

AU - Frings-Meuthen, Petra

AU - Dahlerup, Jens F

AU - Thiel, Steffen

PY - 2014/8

Y1 - 2014/8

N2 - BACKGROUND: Spaceflight studies and ground-based analogues of microgravity indicate a weakening of human immunity. Mannan-binding lectin (MBL) and H-, L-, and M-ficolin together constitute the lectin pathway and mediate the clearance of pathogens through complement activation. We hypothesized that simulated microgravity may weaken human innate immune functions and studied the impact of 6° head-down tilted bed rest (HDT) for 21 d on MBL and ficolin levels.METHODS: Within a 6-mo period, seven men underwent two periods of HDT. Blood samples were analyzed for MBL, H-, L-, and M-ficolin, mannose-binding lectin-associated protein of 44 kDa (MAp44), and collectin liver 1 (CL-L1) by time-resolved immunofluorometric assays (TRIFMA).RESULTS: We observed well-defined individual preintervention levels of MBL and ficolins. Remarkably similar intraindividual changes occurred for MBL and MBL levels decreased (mean 282 ng · ml(-1)) in the recovery phase. Conversely, CL-L1, a protein with MBL-like properties, increased (mean 102 ng · ml(-1)) during the recovery phase. M-ficolin increased (mean 79 ng · ml(-1)) within the first 2 d of HDT, followed by a decrease (mean 112 ng · ml(-1)) during the recovery phase. L-ficolin increased (mean 304 ng · ml(-1)) during HDT, while H-ficolin was essentially unaffected. MAp44, a down-regulator of the lectin pathway, decreased initially (mean 78 ng · ml(-1)) in the recovery phase followed by an increase (mean 131 ng · ml(-1)).DISCUSSION: Alterations in MBL and ficolin levels were modest and with our current knowledge do not lead to overt immunodeficiency. Pronounced changes occurred when the subjects resumed the upright position. In selected individuals, these changes appear to be a conserved response to HDT.

AB - BACKGROUND: Spaceflight studies and ground-based analogues of microgravity indicate a weakening of human immunity. Mannan-binding lectin (MBL) and H-, L-, and M-ficolin together constitute the lectin pathway and mediate the clearance of pathogens through complement activation. We hypothesized that simulated microgravity may weaken human innate immune functions and studied the impact of 6° head-down tilted bed rest (HDT) for 21 d on MBL and ficolin levels.METHODS: Within a 6-mo period, seven men underwent two periods of HDT. Blood samples were analyzed for MBL, H-, L-, and M-ficolin, mannose-binding lectin-associated protein of 44 kDa (MAp44), and collectin liver 1 (CL-L1) by time-resolved immunofluorometric assays (TRIFMA).RESULTS: We observed well-defined individual preintervention levels of MBL and ficolins. Remarkably similar intraindividual changes occurred for MBL and MBL levels decreased (mean 282 ng · ml(-1)) in the recovery phase. Conversely, CL-L1, a protein with MBL-like properties, increased (mean 102 ng · ml(-1)) during the recovery phase. M-ficolin increased (mean 79 ng · ml(-1)) within the first 2 d of HDT, followed by a decrease (mean 112 ng · ml(-1)) during the recovery phase. L-ficolin increased (mean 304 ng · ml(-1)) during HDT, while H-ficolin was essentially unaffected. MAp44, a down-regulator of the lectin pathway, decreased initially (mean 78 ng · ml(-1)) in the recovery phase followed by an increase (mean 131 ng · ml(-1)).DISCUSSION: Alterations in MBL and ficolin levels were modest and with our current knowledge do not lead to overt immunodeficiency. Pronounced changes occurred when the subjects resumed the upright position. In selected individuals, these changes appear to be a conserved response to HDT.

U2 - 10.3357/ASEM.3915.2014

DO - 10.3357/ASEM.3915.2014

M3 - Journal article

C2 - 25199121

VL - 85

SP - 805

EP - 811

JO - Aviation, Space, and Environmental Medicine

JF - Aviation, Space, and Environmental Medicine

SN - 0095-6562

IS - 8

ER -