We investigated the association between the amount of alcohol-based hand rub (hereafter, "hand rub") used and the incidence of healthcare-acquired bloodstream infection (HCR-BSI) from 2004 through early 2008. The amount of hand rub used increased significantly, and the incidence of HCR-BSI remained stable. There was no significant association between the amount of hand rub used and the incidence of HCR-BSI.