Laboratory Technichian. Employed since 1994.
Within the past year I have worked on the following projects:
- Milk quality – importance of somatic cells
- The protein profile of milk
- Toxido
My fields of activity are for the present:
Fractionation of milk
Determination of plasmin activity
Determination of free amino groups (Fluram)
Determination of casein micelles (Master sizer)
Immunological detection of enzymes (Western blotting)
Expert in TOXIDO – running in – start-up
Previous fields of activities:
Protein oxidation in animal raw material:
- Including radical transmissions between proteins made in different model systems using heme proteins, (metmyoglobin, horse radish peroxidase, lacto peroxidase)
- Running-in/carrying out different methods for determination of proteinoxidation
- Dityrosin (HPLC)
- Carbonyl groups (HPLC)
- Freeze-quench electron spin resonance for direct detection of radicals (ESR)
- Determination of antioxidants
Enzyme activities (on Elisa reader):
- Lactoperoxidase
- Glutathione peroxidase (GSH)
- Lactate dehydrogenase (LDH)
- Catalase
- Superoxide Dismutase (SOD)
Other methods:
- Electroforese (one-dimensional and two-dimensional)
- Erythrocyte stability (Elisa reader)
- Histology: Image analysis colouring
I have experience in working with the following equipment:
- HPLC – Varian – Star
- HPLC – HP-Chemstation
- FPLC – Äkta
- Elisareader – KC4
- Spectrophotometer – HP
- Fluorometer
- Others
- Training of students and laboratory worker trainees
- Sample pre-treatment in stable and slaughterhouse
- Data processing, Excel