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Steen Vang Petersen

Expression of single-chain variable fragments fused with the Fc-region of rabbit IgG in Leishmania tarentolae

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research

Standard

Expression of single-chain variable fragments fused with the Fc-region of rabbit IgG in Leishmania tarentolae. / Jørgensen, Mathias Lindh; Friis, Niels Anton; Just, Jesper et al.
In: Microbial Cell Factories, Vol. 13, No. 1, 15.01.2014, p. 9.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research

Harvard

Jørgensen, ML, Friis, NA, Just, J, Madsen, P, Petersen, SV & Kristensen, P 2014, 'Expression of single-chain variable fragments fused with the Fc-region of rabbit IgG in Leishmania tarentolae', Microbial Cell Factories, vol. 13, no. 1, pp. 9. https://doi.org/10.1186/1475-2859-13-9

APA

Jørgensen, M. L., Friis, N. A., Just, J., Madsen, P., Petersen, S. V., & Kristensen, P. (2014). Expression of single-chain variable fragments fused with the Fc-region of rabbit IgG in Leishmania tarentolae. Microbial Cell Factories, 13(1), 9. https://doi.org/10.1186/1475-2859-13-9

CBE

Jørgensen ML, Friis NA, Just J, Madsen P, Petersen SV, Kristensen P. 2014. Expression of single-chain variable fragments fused with the Fc-region of rabbit IgG in Leishmania tarentolae. Microbial Cell Factories. 13(1):9. https://doi.org/10.1186/1475-2859-13-9

MLA

Jørgensen, Mathias Lindh et al. "Expression of single-chain variable fragments fused with the Fc-region of rabbit IgG in Leishmania tarentolae". Microbial Cell Factories. 2014, 13(1). 9. https://doi.org/10.1186/1475-2859-13-9

Vancouver

Jørgensen ML, Friis NA, Just J, Madsen P, Petersen SV, Kristensen P. Expression of single-chain variable fragments fused with the Fc-region of rabbit IgG in Leishmania tarentolae. Microbial Cell Factories. 2014 Jan 15;13(1):9. doi: 10.1186/1475-2859-13-9

Author

Jørgensen, Mathias Lindh ; Friis, Niels Anton ; Just, Jesper et al. / Expression of single-chain variable fragments fused with the Fc-region of rabbit IgG in Leishmania tarentolae. In: Microbial Cell Factories. 2014 ; Vol. 13, No. 1. pp. 9.

Bibtex

@article{3de9e0939cf84163ae0fa70e69a0e309,
title = "Expression of single-chain variable fragments fused with the Fc-region of rabbit IgG in Leishmania tarentolae",
abstract = "In recent years the generation of antibodies by recombinant methods, such as phage display technology, has increased the speed by which antibodies can be obtained. However, in some cases when recombinant antibodies have to be validated, expression in E. coli can be problematic. This primarily occurs when codon usage or protein folding of specific antibody fragments is incompatible with the E. coli translation and folding machinery, for instance when recombinant antibody formats that include the Fc-region are needed. In such cases other expression systems can be used, including the protozoan parasite Leishmania tarentolae (L. tarentolae). This novel host for recombinant protein expression has recently shown promising properties for the expression of single-chain antibody fragments. We have utilised the L. tarentolae T7-TR system to achieve expression and secretion of two scFvs fused to the Fc-region of rabbit immunoglobulin G (IgG).",
author = "J{\o}rgensen, {Mathias Lindh} and Friis, {Niels Anton} and Jesper Just and Peder Madsen and Petersen, {Steen Vang} and Peter Kristensen",
year = "2014",
month = jan,
day = "15",
doi = "10.1186/1475-2859-13-9",
language = "English",
volume = "13",
pages = "9",
journal = "Microbial Cell Factories",
issn = "1475-2859",
publisher = "BioMed Central",
number = "1",

}

RIS

TY - JOUR

T1 - Expression of single-chain variable fragments fused with the Fc-region of rabbit IgG in Leishmania tarentolae

AU - Jørgensen, Mathias Lindh

AU - Friis, Niels Anton

AU - Just, Jesper

AU - Madsen, Peder

AU - Petersen, Steen Vang

AU - Kristensen, Peter

PY - 2014/1/15

Y1 - 2014/1/15

N2 - In recent years the generation of antibodies by recombinant methods, such as phage display technology, has increased the speed by which antibodies can be obtained. However, in some cases when recombinant antibodies have to be validated, expression in E. coli can be problematic. This primarily occurs when codon usage or protein folding of specific antibody fragments is incompatible with the E. coli translation and folding machinery, for instance when recombinant antibody formats that include the Fc-region are needed. In such cases other expression systems can be used, including the protozoan parasite Leishmania tarentolae (L. tarentolae). This novel host for recombinant protein expression has recently shown promising properties for the expression of single-chain antibody fragments. We have utilised the L. tarentolae T7-TR system to achieve expression and secretion of two scFvs fused to the Fc-region of rabbit immunoglobulin G (IgG).

AB - In recent years the generation of antibodies by recombinant methods, such as phage display technology, has increased the speed by which antibodies can be obtained. However, in some cases when recombinant antibodies have to be validated, expression in E. coli can be problematic. This primarily occurs when codon usage or protein folding of specific antibody fragments is incompatible with the E. coli translation and folding machinery, for instance when recombinant antibody formats that include the Fc-region are needed. In such cases other expression systems can be used, including the protozoan parasite Leishmania tarentolae (L. tarentolae). This novel host for recombinant protein expression has recently shown promising properties for the expression of single-chain antibody fragments. We have utilised the L. tarentolae T7-TR system to achieve expression and secretion of two scFvs fused to the Fc-region of rabbit immunoglobulin G (IgG).

U2 - 10.1186/1475-2859-13-9

DO - 10.1186/1475-2859-13-9

M3 - Journal article

C2 - 24428896

VL - 13

SP - 9

JO - Microbial Cell Factories

JF - Microbial Cell Factories

SN - 1475-2859

IS - 1

ER -