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Søren Lykke-Andersen

RNA Exosome Depletion Reveals Transcription Upstream of Active Human Promoters

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  • Bioinformatics Research Centre (BiRC)
  • Department of Molecular Biology
  • Department of Biological Sciences, Genetics and Ecology
Studies have shown that the bulk of eukaryotic genomes is transcribed. Transcriptome maps are frequently updated, but low-abundant transcripts have likely gone unnoticed. To eliminate RNA degradation, we depleted the exonucleolytic RNA exosome from human cells and then subjected the RNA to tiling microarray analysis. This revealed a class of short, polyadenylated and highly unstable RNAs. These promoter upstream transcripts (PROMPTs) are produced about 0.5 to 2.5 kb upstream of active transcription start sites (TSSs). PROMPT transcription occurs in both sense and antisense directions with respect to the downstream gene. In addition, it requires the presence of the gene promoter, and is positively correlated with gene activity. We propose that PROMPT transcription is a common characteristic of RNA polymerase II (RNAPII) transcribed genes with a possible regulatory potential.
Original languageEnglish
JournalScience
Volume322
Issue5909
Pages (from-to)1851-1854
Number of pages4
ISSN0036-8075
DOIs
Publication statusPublished - 19 Dec 2008

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