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Søren Lykke-Andersen

Identification of a Nuclear Exosome Decay Pathway for Processed Transcripts

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Identification of a Nuclear Exosome Decay Pathway for Processed Transcripts. / Meola, Nicola; Domanski, Michal; Karadoulama, Evdoxia; Chen, Yun; Gentil, Coline; Pultz, Dennis; Vitting-Seerup, Kristoffer; Lykke-Andersen, Søren; Andersen, Jens S; Sandelin, Albin; Jensen, Torben Heick.

In: Molecular Cell, Vol. 64, No. 3, 03.11.2016, p. 520-533.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Meola, N, Domanski, M, Karadoulama, E, Chen, Y, Gentil, C, Pultz, D, Vitting-Seerup, K, Lykke-Andersen, S, Andersen, JS, Sandelin, A & Jensen, TH 2016, 'Identification of a Nuclear Exosome Decay Pathway for Processed Transcripts', Molecular Cell, vol. 64, no. 3, pp. 520-533. https://doi.org/10.1016/j.molcel.2016.09.025

APA

CBE

Meola N, Domanski M, Karadoulama E, Chen Y, Gentil C, Pultz D, Vitting-Seerup K, Lykke-Andersen S, Andersen JS, Sandelin A, Jensen TH. 2016. Identification of a Nuclear Exosome Decay Pathway for Processed Transcripts. Molecular Cell. 64(3):520-533. https://doi.org/10.1016/j.molcel.2016.09.025

MLA

Vancouver

Meola N, Domanski M, Karadoulama E, Chen Y, Gentil C, Pultz D et al. Identification of a Nuclear Exosome Decay Pathway for Processed Transcripts. Molecular Cell. 2016 Nov 3;64(3):520-533. https://doi.org/10.1016/j.molcel.2016.09.025

Author

Meola, Nicola ; Domanski, Michal ; Karadoulama, Evdoxia ; Chen, Yun ; Gentil, Coline ; Pultz, Dennis ; Vitting-Seerup, Kristoffer ; Lykke-Andersen, Søren ; Andersen, Jens S ; Sandelin, Albin ; Jensen, Torben Heick. / Identification of a Nuclear Exosome Decay Pathway for Processed Transcripts. In: Molecular Cell. 2016 ; Vol. 64, No. 3. pp. 520-533.

Bibtex

@article{9b05dc938db6462e932c54b51e994132,
title = "Identification of a Nuclear Exosome Decay Pathway for Processed Transcripts",
abstract = "The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its co-factor Mtr4p/hMTR4, which links to RNA-binding protein adaptors. One example is the trimeric human nuclear exosome targeting (NEXT) complex, which is composed of hMTR4, the Zn-finger protein ZCCHC8, and the RNA-binding factor RBM7. NEXT primarily targets early and unprocessed transcripts, which demands a rationale for how the nuclear exosome recognizes processed RNAs. Here, we describe the poly(A) tail exosome targeting (PAXT) connection, which comprises the ZFC3H1 Zn-knuckle protein as a central link between hMTR4 and the nuclear poly(A)-binding protein PABPN1. Individual depletion of ZFC3H1 and PABPN1 results in the accumulation of common transcripts that are generally both longer and more extensively polyadenylated than NEXT substrates. Importantly, ZFC3H1/PABPN1 and ZCCHC8/RBM7 contact hMTR4 in a mutually exclusive manner, revealing that the exosome targets nuclear transcripts of different maturation status by substituting its hMTR4-associating adaptors.",
author = "Nicola Meola and Michal Domanski and Evdoxia Karadoulama and Yun Chen and Coline Gentil and Dennis Pultz and Kristoffer Vitting-Seerup and S{\o}ren Lykke-Andersen and Andersen, {Jens S} and Albin Sandelin and Jensen, {Torben Heick}",
note = "Copyright {\circledC} 2016 Elsevier Inc. All rights reserved.",
year = "2016",
month = "11",
day = "3",
doi = "10.1016/j.molcel.2016.09.025",
language = "English",
volume = "64",
pages = "520--533",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "3",

}

RIS

TY - JOUR

T1 - Identification of a Nuclear Exosome Decay Pathway for Processed Transcripts

AU - Meola, Nicola

AU - Domanski, Michal

AU - Karadoulama, Evdoxia

AU - Chen, Yun

AU - Gentil, Coline

AU - Pultz, Dennis

AU - Vitting-Seerup, Kristoffer

AU - Lykke-Andersen, Søren

AU - Andersen, Jens S

AU - Sandelin, Albin

AU - Jensen, Torben Heick

N1 - Copyright © 2016 Elsevier Inc. All rights reserved.

PY - 2016/11/3

Y1 - 2016/11/3

N2 - The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its co-factor Mtr4p/hMTR4, which links to RNA-binding protein adaptors. One example is the trimeric human nuclear exosome targeting (NEXT) complex, which is composed of hMTR4, the Zn-finger protein ZCCHC8, and the RNA-binding factor RBM7. NEXT primarily targets early and unprocessed transcripts, which demands a rationale for how the nuclear exosome recognizes processed RNAs. Here, we describe the poly(A) tail exosome targeting (PAXT) connection, which comprises the ZFC3H1 Zn-knuckle protein as a central link between hMTR4 and the nuclear poly(A)-binding protein PABPN1. Individual depletion of ZFC3H1 and PABPN1 results in the accumulation of common transcripts that are generally both longer and more extensively polyadenylated than NEXT substrates. Importantly, ZFC3H1/PABPN1 and ZCCHC8/RBM7 contact hMTR4 in a mutually exclusive manner, revealing that the exosome targets nuclear transcripts of different maturation status by substituting its hMTR4-associating adaptors.

AB - The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its co-factor Mtr4p/hMTR4, which links to RNA-binding protein adaptors. One example is the trimeric human nuclear exosome targeting (NEXT) complex, which is composed of hMTR4, the Zn-finger protein ZCCHC8, and the RNA-binding factor RBM7. NEXT primarily targets early and unprocessed transcripts, which demands a rationale for how the nuclear exosome recognizes processed RNAs. Here, we describe the poly(A) tail exosome targeting (PAXT) connection, which comprises the ZFC3H1 Zn-knuckle protein as a central link between hMTR4 and the nuclear poly(A)-binding protein PABPN1. Individual depletion of ZFC3H1 and PABPN1 results in the accumulation of common transcripts that are generally both longer and more extensively polyadenylated than NEXT substrates. Importantly, ZFC3H1/PABPN1 and ZCCHC8/RBM7 contact hMTR4 in a mutually exclusive manner, revealing that the exosome targets nuclear transcripts of different maturation status by substituting its hMTR4-associating adaptors.

U2 - 10.1016/j.molcel.2016.09.025

DO - 10.1016/j.molcel.2016.09.025

M3 - Journal article

VL - 64

SP - 520

EP - 533

JO - Molecular Cell

JF - Molecular Cell

SN - 1097-2765

IS - 3

ER -