Søren Kragh Moestrup

The macrophage-related biomarkers sCD163 and sCD206 are released by different shedding mechanisms

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The macrophage-related biomarkers sCD163 and sCD206 are released by different shedding mechanisms. / Nielsen, Marlene Christina; Andersen, Morten Nørgaard; Rittig, Nikolaj; Rødgaard-Hansen, Sidsel; Grønbaek, Henning; Moestrup, Søren Kragh; Møller, Holger Jon; Etzerodt, Anders.

In: Journal of Leukocyte Biology, Vol. 106, No. 5, 11.2019, p. 1129-1138.

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@article{3758e6b1933f4b5dbf584621f8e7220f,
title = "The macrophage-related biomarkers sCD163 and sCD206 are released by different shedding mechanisms",
abstract = "The hemoglobin receptor CD163 and the mannose receptor CD206 are both expressed on the surface of human macrophages. Upon inflammatory activation, the receptors are shed from the macrophage surface generating soluble products. The plasma concentration of both soluble CD163 (sCD163) and soluble CD206 (sCD206) are increased in several diseases, including inflammatory conditions and cancer. Here, we show that in contrast to CD163, LPS-mediated shedding of CD206 in humans is slow and a result of indirect signaling. Although both sCD163 and sCD206 were increased in response to LPS stimulation in vivo, only CD163 was shed from LPS-stimulated macrophages in vitro. Although both sCD163 and sCD206 were released from cultured macrophages stimulated with zymosan and PMA, shedding of CD206 was generally slower and less efficient and not reduced by inhibitors against the major protease classes. These data indicate that CD163 and CD206 are shed from the macrophages by very different mechanisms potentially involving distinctive inflammatory processes.",
keywords = "LPS, PKC, PMA, Zymosan, protease inhibitor",
author = "Nielsen, {Marlene Christina} and Andersen, {Morten N{\o}rgaard} and Nikolaj Rittig and Sidsel R{\o}dgaard-Hansen and Henning Gr{\o}nbaek and Moestrup, {S{\o}ren Kragh} and M{\o}ller, {Holger Jon} and Anders Etzerodt",
note = "{\textcopyright}2019 Society for Leukocyte Biology.",
year = "2019",
month = nov,
doi = "10.1002/JLB.3A1218-500R",
language = "English",
volume = "106",
pages = "1129--1138",
journal = "Journal of Leukocyte Biology",
issn = "0741-5400",
publisher = "Federation of American Societies for Experimental Biology",
number = "5",

}

RIS

TY - JOUR

T1 - The macrophage-related biomarkers sCD163 and sCD206 are released by different shedding mechanisms

AU - Nielsen, Marlene Christina

AU - Andersen, Morten Nørgaard

AU - Rittig, Nikolaj

AU - Rødgaard-Hansen, Sidsel

AU - Grønbaek, Henning

AU - Moestrup, Søren Kragh

AU - Møller, Holger Jon

AU - Etzerodt, Anders

N1 - ©2019 Society for Leukocyte Biology.

PY - 2019/11

Y1 - 2019/11

N2 - The hemoglobin receptor CD163 and the mannose receptor CD206 are both expressed on the surface of human macrophages. Upon inflammatory activation, the receptors are shed from the macrophage surface generating soluble products. The plasma concentration of both soluble CD163 (sCD163) and soluble CD206 (sCD206) are increased in several diseases, including inflammatory conditions and cancer. Here, we show that in contrast to CD163, LPS-mediated shedding of CD206 in humans is slow and a result of indirect signaling. Although both sCD163 and sCD206 were increased in response to LPS stimulation in vivo, only CD163 was shed from LPS-stimulated macrophages in vitro. Although both sCD163 and sCD206 were released from cultured macrophages stimulated with zymosan and PMA, shedding of CD206 was generally slower and less efficient and not reduced by inhibitors against the major protease classes. These data indicate that CD163 and CD206 are shed from the macrophages by very different mechanisms potentially involving distinctive inflammatory processes.

AB - The hemoglobin receptor CD163 and the mannose receptor CD206 are both expressed on the surface of human macrophages. Upon inflammatory activation, the receptors are shed from the macrophage surface generating soluble products. The plasma concentration of both soluble CD163 (sCD163) and soluble CD206 (sCD206) are increased in several diseases, including inflammatory conditions and cancer. Here, we show that in contrast to CD163, LPS-mediated shedding of CD206 in humans is slow and a result of indirect signaling. Although both sCD163 and sCD206 were increased in response to LPS stimulation in vivo, only CD163 was shed from LPS-stimulated macrophages in vitro. Although both sCD163 and sCD206 were released from cultured macrophages stimulated with zymosan and PMA, shedding of CD206 was generally slower and less efficient and not reduced by inhibitors against the major protease classes. These data indicate that CD163 and CD206 are shed from the macrophages by very different mechanisms potentially involving distinctive inflammatory processes.

KW - LPS

KW - PKC

KW - PMA

KW - Zymosan

KW - protease inhibitor

UR - http://www.scopus.com/inward/record.url?scp=85068160191&partnerID=8YFLogxK

U2 - 10.1002/JLB.3A1218-500R

DO - 10.1002/JLB.3A1218-500R

M3 - Journal article

C2 - 31242338

VL - 106

SP - 1129

EP - 1138

JO - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

SN - 0741-5400

IS - 5

ER -