Søren Kragh Moestrup

Characterization of Microfibrillar-associated Protein 4 (MFAP4) as a Tropoelastin- and Fibrillin-binding Protein Involved in Elastic Fiber Formation

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Characterization of Microfibrillar-associated Protein 4 (MFAP4) as a Tropoelastin- and Fibrillin-binding Protein Involved in Elastic Fiber Formation. / Pilecki, Bartosz; Holm, Anne T; Schlosser, Anders; Moeller, Jesper B; Wohl, Alexander P; Zuk, Alexandra V; Heumüller, Stefanie E; Wallis, Russell; Moestrup, Soren K; Sengle, Gerhard; Holmskov, Uffe; Sorensen, Grith L.

In: The Journal of biological chemistry, Vol. 291, No. 3, 15.01.2016, p. 1103-14.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Pilecki, B, Holm, AT, Schlosser, A, Moeller, JB, Wohl, AP, Zuk, AV, Heumüller, SE, Wallis, R, Moestrup, SK, Sengle, G, Holmskov, U & Sorensen, GL 2016, 'Characterization of Microfibrillar-associated Protein 4 (MFAP4) as a Tropoelastin- and Fibrillin-binding Protein Involved in Elastic Fiber Formation', The Journal of biological chemistry, vol. 291, no. 3, pp. 1103-14. https://doi.org/10.1074/jbc.M115.681775

APA

Pilecki, B., Holm, A. T., Schlosser, A., Moeller, J. B., Wohl, A. P., Zuk, A. V., Heumüller, S. E., Wallis, R., Moestrup, S. K., Sengle, G., Holmskov, U., & Sorensen, G. L. (2016). Characterization of Microfibrillar-associated Protein 4 (MFAP4) as a Tropoelastin- and Fibrillin-binding Protein Involved in Elastic Fiber Formation. The Journal of biological chemistry, 291(3), 1103-14. https://doi.org/10.1074/jbc.M115.681775

CBE

Pilecki B, Holm AT, Schlosser A, Moeller JB, Wohl AP, Zuk AV, Heumüller SE, Wallis R, Moestrup SK, Sengle G, Holmskov U, Sorensen GL. 2016. Characterization of Microfibrillar-associated Protein 4 (MFAP4) as a Tropoelastin- and Fibrillin-binding Protein Involved in Elastic Fiber Formation. The Journal of biological chemistry. 291(3):1103-14. https://doi.org/10.1074/jbc.M115.681775

MLA

Vancouver

Pilecki B, Holm AT, Schlosser A, Moeller JB, Wohl AP, Zuk AV et al. Characterization of Microfibrillar-associated Protein 4 (MFAP4) as a Tropoelastin- and Fibrillin-binding Protein Involved in Elastic Fiber Formation. The Journal of biological chemistry. 2016 Jan 15;291(3):1103-14. https://doi.org/10.1074/jbc.M115.681775

Author

Pilecki, Bartosz ; Holm, Anne T ; Schlosser, Anders ; Moeller, Jesper B ; Wohl, Alexander P ; Zuk, Alexandra V ; Heumüller, Stefanie E ; Wallis, Russell ; Moestrup, Soren K ; Sengle, Gerhard ; Holmskov, Uffe ; Sorensen, Grith L. / Characterization of Microfibrillar-associated Protein 4 (MFAP4) as a Tropoelastin- and Fibrillin-binding Protein Involved in Elastic Fiber Formation. In: The Journal of biological chemistry. 2016 ; Vol. 291, No. 3. pp. 1103-14.

Bibtex

@article{e91f79b3f63346329860b97beb842038,
title = "Characterization of Microfibrillar-associated Protein 4 (MFAP4) as a Tropoelastin- and Fibrillin-binding Protein Involved in Elastic Fiber Formation",
abstract = "MFAP4 (microfibrillar-associated protein 4) is an extracellular glycoprotein found in elastic fibers without a clearly defined role in elastic fiber assembly. In the present study, we characterized molecular interactions between MFAP4 and elastic fiber components. We established that MFAP4 primarily assembles into trimeric and hexameric structures of homodimers. Binding analysis revealed that MFAP4 specifically binds tropoelastin and fibrillin-1 and -2, as well as the elastin cross-linking amino acid desmosine, and that it co-localizes with fibrillin-1-positive fibers in vivo. Site-directed mutagenesis disclosed residues Phe(241) and Ser(203) in MFAP4 as being crucial for type I collagen, elastin, and tropoelastin binding. Furthermore, we found that MFAP4 actively promotes tropoelastin self-assembly. In conclusion, our data identify MFAP4 as a new ligand of microfibrils and tropoelastin involved in proper elastic fiber organization.",
keywords = "Amino Acid Substitution, Animals, Carrier Proteins, Desmosine, Elastic Tissue, Extracellular Matrix Proteins, Fibrillin-1, Fibrillins, Glycoproteins, Humans, Ligands, Male, Mice, Inbred C57BL, Mice, Mutant Strains, Microfibrils, Microfilament Proteins, Models, Molecular, Mutation, Peptide Fragments, Protein Interaction Domains and Motifs, Protein Isoforms, Protein Multimerization, Protein Transport, Recombinant Proteins, Tropoelastin, Journal Article, Research Support, Non-U.S. Gov't",
author = "Bartosz Pilecki and Holm, {Anne T} and Anders Schlosser and Moeller, {Jesper B} and Wohl, {Alexander P} and Zuk, {Alexandra V} and Heum{\"u}ller, {Stefanie E} and Russell Wallis and Moestrup, {Soren K} and Gerhard Sengle and Uffe Holmskov and Sorensen, {Grith L}",
note = "{\textcopyright} 2016 by The American Society for Biochemistry and Molecular Biology, Inc.",
year = "2016",
month = jan,
day = "15",
doi = "10.1074/jbc.M115.681775",
language = "English",
volume = "291",
pages = "1103--14",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "3",

}

RIS

TY - JOUR

T1 - Characterization of Microfibrillar-associated Protein 4 (MFAP4) as a Tropoelastin- and Fibrillin-binding Protein Involved in Elastic Fiber Formation

AU - Pilecki, Bartosz

AU - Holm, Anne T

AU - Schlosser, Anders

AU - Moeller, Jesper B

AU - Wohl, Alexander P

AU - Zuk, Alexandra V

AU - Heumüller, Stefanie E

AU - Wallis, Russell

AU - Moestrup, Soren K

AU - Sengle, Gerhard

AU - Holmskov, Uffe

AU - Sorensen, Grith L

N1 - © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

PY - 2016/1/15

Y1 - 2016/1/15

N2 - MFAP4 (microfibrillar-associated protein 4) is an extracellular glycoprotein found in elastic fibers without a clearly defined role in elastic fiber assembly. In the present study, we characterized molecular interactions between MFAP4 and elastic fiber components. We established that MFAP4 primarily assembles into trimeric and hexameric structures of homodimers. Binding analysis revealed that MFAP4 specifically binds tropoelastin and fibrillin-1 and -2, as well as the elastin cross-linking amino acid desmosine, and that it co-localizes with fibrillin-1-positive fibers in vivo. Site-directed mutagenesis disclosed residues Phe(241) and Ser(203) in MFAP4 as being crucial for type I collagen, elastin, and tropoelastin binding. Furthermore, we found that MFAP4 actively promotes tropoelastin self-assembly. In conclusion, our data identify MFAP4 as a new ligand of microfibrils and tropoelastin involved in proper elastic fiber organization.

AB - MFAP4 (microfibrillar-associated protein 4) is an extracellular glycoprotein found in elastic fibers without a clearly defined role in elastic fiber assembly. In the present study, we characterized molecular interactions between MFAP4 and elastic fiber components. We established that MFAP4 primarily assembles into trimeric and hexameric structures of homodimers. Binding analysis revealed that MFAP4 specifically binds tropoelastin and fibrillin-1 and -2, as well as the elastin cross-linking amino acid desmosine, and that it co-localizes with fibrillin-1-positive fibers in vivo. Site-directed mutagenesis disclosed residues Phe(241) and Ser(203) in MFAP4 as being crucial for type I collagen, elastin, and tropoelastin binding. Furthermore, we found that MFAP4 actively promotes tropoelastin self-assembly. In conclusion, our data identify MFAP4 as a new ligand of microfibrils and tropoelastin involved in proper elastic fiber organization.

KW - Amino Acid Substitution

KW - Animals

KW - Carrier Proteins

KW - Desmosine

KW - Elastic Tissue

KW - Extracellular Matrix Proteins

KW - Fibrillin-1

KW - Fibrillins

KW - Glycoproteins

KW - Humans

KW - Ligands

KW - Male

KW - Mice, Inbred C57BL

KW - Mice, Mutant Strains

KW - Microfibrils

KW - Microfilament Proteins

KW - Models, Molecular

KW - Mutation

KW - Peptide Fragments

KW - Protein Interaction Domains and Motifs

KW - Protein Isoforms

KW - Protein Multimerization

KW - Protein Transport

KW - Recombinant Proteins

KW - Tropoelastin

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1074/jbc.M115.681775

DO - 10.1074/jbc.M115.681775

M3 - Journal article

C2 - 26601954

VL - 291

SP - 1103

EP - 1114

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 3

ER -