Aarhus University Seal / Aarhus Universitets segl

Ricarda Margarete Engberg

Veterinary inspector , Associate Professor

Profile photoRicarda Margarete Engberg
Associate professor
Department of Animal Science
Blichers Allé 20
8830 Tjele
Email: ricarda.engberg@anis.au.dk
Phone: +4587157904
Web: https://pure.au.dk/portal/en/persons/ricarda-margarete-engberg(a50303d7-b723-4f86-8f6a-6bf787cdaa3b)/cv.html?id=34827055


The area of research is focused on the microbial fermentation in feed and in the digestive tract of monogastric animals (pigs and poultry) with special reference to broilers.The research work includes studies on the composition of the natural intestinal microflora as well as the dietary factors influencing the microbial development and fermentation in the digestive tract. The aim of the work is to improve animal growth and gastrointestinal health without the use of antibiotic growth promoters. The focus of interest is to find feeding strategies in order to strengthen the natural microflora by addition of yeast or bacterial cultures or other substances like feeding enzymes, organic acids and specific carbohydrates etc.

The methods used in our laboratory include among others anaerobic culturing of bacteria, determination of the microbial activity in the gastrointestinal tract, quantitative analysis of bacterial fermentation products (including short chain fatty acids (SCAFA) and gas

1986: Cand. med. vet. Ludwig-Maximilians-Universität (LMU) Munich, Germany
1988: Dr. med. vet. (LMU) Munich. Germany

01/09 1988-31/10 1988: Meat Control at the municipal slaughterhouse of Aarhus, Denmark.
01/11 1988-31/12 1996: Scientific assistent at the Department of Animal Physiology and Bio¬chemistry, National Institute of Animal Science, Research Centre Foulum, Denmark.
01/01 1997-31/12 2010: Senior Research Scientist, Dept. of Animal Science, Faculty of Science and Technology, AU.01/01 2011-: Associate Professor, Dept. of Animal Science, Faculty of Science and Technology, AU.

Research profile
Composition and activity of the natural intestinal microbiota in relation to dietary factors. Feeding and management strategies strengthening the natural microbiota and the gut associated immune function (fermented feed, probiotics, feeding enzymes, organic acids, specific carbohydrates and plant extracts). Focus on dietary factors that support gastro-intestinal health and suppress the abundance of zoonotic bacteria as well as bird pathogenic bacteria and parasites.
•Author/co-author of peer review articles in international journals: 76
•Number of contributions to international conferences, proceedings and abstracts: 73
•H-index (11/2 2019):, Web of Science: 23, Google Scholar: 26, No. of citations: 2241
•Responsible supervisor and of master and PhD.-students 

Current research collaborations
•Collaboration with the University of Poznan (Poland) on the use of insects in feed for poultry
•Collaboration with France, Netherlands, Belgium, Italy, Finland, Germany and Romania. in an EU project (Horizont 2020) Poultry and Pig Low-input and Organic production systems’ Welfare (PPILOW)
•Colaboraion with the Technical Institute (Denmark) on the use of insects in monogastric nutrition
•Collaboration with SEGES (Knowledge Transfer, Denmark) and DLG (feed industry) on projects in relation to poultry gut health
•External examiner of master and PhD-students

Research based consulting of authorities
•Use of insects in organic production of pigs and poultry
•Risk assessment of Salmonella presence in stored ground cereals and oil seeds
•Knowledge synthesis on the humane killing of new born piglets with a birth weight below 5 kg
•Knowledge synthesis on the stunning of broilers and ducks with carbondioxide

Leadership and administration
•Head of the Master`s program in Agrobiology, AU
•Member of the Teaching Board at the Dept.of Animal Science, AU
•Inspecting veterinarian in the stable facilities at the Dept. of Animal Science, AU
•Member of the Animal Welfare Body at the Dept.of Animal Science, AU
•Chair and member of scientific evaluation commitees for positions as associate professor, assistant professor, post doc and PhD.
•Member of the Editorial board Acta Agriculturae Scandinavica, Section A – Animal Science.
•Ad hoc referee for the following journals: Animal Feed Science and Technology, Poultry Science, British Poultry Science, The Journal of Nutrition, Journal of Animal and Feed Sciences, Animal, Avian pathology, Avian Diseases, Applied and Environmental Microbiology
•Member of the World´s Poultry Science Association 

•Responsible for a 10 ECTS course in Livestock Diseases and Disease Prevention (MSc)
•Responsible for a 5 ECTS course in Animal Hygiene and Diseases (BSc)
•Responsible for a Ph.D.-course in Poultry Production and Health
•Responsible for a Ph.D.-course in Animal Experimentation (Category B)
•Contribution with lectures and practicals to the courses Comparative Anatomy and Physiology (BSc), Animal nutrition (BSc), Quantitative Nutrion and Physiology (MSc), Animal Production Practices (MSc)

Innovation, Industry collaboration and Technology Transfer
•Collaboration with Malcisbo (Swizerland) on the development of a carbohydrate based vaccine against Campylobacter jejuni for broilers.
•Collaboration with Force Technology (Denmark) on the evaluation of Aerocollect, a device measuring the presence of Salmonella in stable air samples.
•Collaboration with Adisseo (France) evaluating the effect of a probiotic against necrotic enteritis in broilers.

Research outputs from the last five years (peer reviewed)

A new tool for air sample-based surveillance of Campylobacter and Salmonella in poultry flocks

Andersen, K. B., Engberg, R. M. & Skov, J., Jun 2022, In: Journal of Applied Poultry Research. 31, 2, 12 p., 100236.

Impact of Dietary Sodium Butyrate and Salinomycin on Performance and Intestinal Microbiota in a Broiler Gut Leakage Model

Naghizadeh, M., Klaver, L., Schönherz, A. A., Rani, S., Dalgaard, T. S. & Engberg, R. M., Jan 2022, In: Animals. 12, 1, 23 p., 111.

Effects of encapsulated butyrate and salinomycin on gut leakage and intestinal inflammation in broilers

Naghizadeh, M., Dalgaard, T. S., Klaver, L. & Engberg, R. M., 2022, (E-pub ahead of print) In: British Poultry Science.

Performance, nutrient digestibility and selected gut health parameters of broilers fed with black soldier fly, lesser mealworm and yellow mealworm

van der Heide, M. E., Nørgaard, J. V. & Engberg, R. M., 2021, In: Journal of Insects as Food and Feed. 7, 6, p. 1011-1022 12 p.

Effect of serum anti-phage activity on colibacillosis control by repeated phage therapy in broilers

Naghizadeh, M., Karimi Torshizi, M. A., Rahimi, S., Engberg, R. M. & Sørensen Dalgaard, T., Jul 2019, In: Veterinary Microbiology. 234, p. 61-71 11 p.

The influence of selenium and selenoproteins on immune responses of poultry and pigs

Dalgaard, T. S., Briens, M., Engberg, R. M. & Lauridsen, C., 1 Apr 2018, In: Animal Feed Science and Technology. 238, p. 73-83 11 p.

Influence of silver nanoparticles on growth and health of broiler chickens after infection with Campylobacter jejuni

Vadalasetty, K. P., Lauridsen, C., Engberg, R. M., Vadalasetty, R., Kutwin, M., Chwalibog, A. & Sawosz, E., 2018, In: B M C Veterinary Research. 14, 11 p.

Influence of feeding crimped kernel maize silage on the course of subclinical necrotic enteritis in a broiler disease model

Sharma, N., Ranjitkar, S., Sharma, N. K. & Engberg, R. M., Dec 2017, In: Animal Nutrition. 3, 4, p. 392-398 7 p.

Insect proteins as a potential source of antimicrobial peptides in livestock production

Józefiak, A. & Engberg, R. M., 15 May 2017, In: Journal of Animal and Feed Sciences. 26, 2, p. 87-99 13 p.

The nisin improves broiler chicken growth performance and interacts with salinomycin in terms of gastrointestinal tract microbiota composition

Kieronczyk, B., Pruszyńska-Oszmałek, E., Swiatkiewicz, S., Rawski, M., Dlugosz, J., Engberg, R. G. & Józefiak, D., 15 Dec 2016, In: Journal of Animal and Feed Sciences. 25, 4, p. 309-316 8 p.

Helminth infection is associated with hen mortality in Danish organic egg production

Hinrichsen, L. K., Labouriau, R., Engberg, R. M., Knierim, U. & Sørensen, J. T., 23 Aug 2016, In: Veterinary Record. 179, 8, p. article 196 5 p.

The influence of feeding crimped kernel maize silage on growth performance and intestinal colonization with Campylobacter jejuni in broilers

Ranjitkar, S. & Engberg, R. G., May 2016, In: Avian Pathology. 45, 2, p. 253-260 8 p.

Bacterial successions in the Broiler Gastrointestinal tract

Ranjitkar, S., Lawley, B., Tannock, G. & Engberg, R. G., Apr 2016, In: Applied and Environmental Microbiology. 82, 8, p. 2399-2410 11 p.

Insects - a natural nutrient source for poultry - a review

Józefiak, D., Josefiak, A., Kieronczyk, B., Rawski, M., Swiatkiewicz, S., Dlugosz, J. & Engberg, R. G., Apr 2016, In: Annals of Animal Science. 16, 2, p. 297-313 17 p.

The influence of Blue mussel (Mytilus edulis) and Starfish (Asterias rubens) meals on production performance, egg quality and apparent total tract digestibility of nutrients of laying hens

Afrose, S., Hammershøj, M., Nørgaard, J. V., Engberg, R. G. & Steenfeldt, S., Mar 2016, In: Animal Feed Science and Technology. 213, p. 108-117 10 p.

Clostridium perfringens challenge and dietary fat type modifies performance, microbiota composition and histomorphology of the broiler chicken gastrointestinal tract

Josefiak, D., Swiatkiewicz, S., Kieronczyk, B., Rawski, M., Dlugosz, J., Engberg, R. G. & Højberg, O., 2016, In: Archiv fuer Gefluegelkunde. 80

The influence of feeding crimped kernel maize silage on broiler production, nutrient digestibility and meat quality

Ranjitkar, S., Karlsson, A. H., Petersen, M. A., Bredie, W. L. P., Petersen, J. S. & Engberg, R. G., 2016, In: British Poultry Science. 57, 1, p. 93-104

Effects of Bos taurus autosome 9-located quantitative trait loci haplotypes on enzymatic mastitis indicators of milk from dairy cows experimentally inoculated with Escherichia coli

Sørensen, L. P., Engberg, R. G., Løvendahl, P. & Larsen, T., Aug 2015, In: Journal of Dairy Science. 98, 8, p. 5440-5447 8 p.

Influence of genotype and feeding strategy on pig performance, plasma concentrations of micro nutrients, immune responses and faecal microbiota composition of growing-finishing pigs in a forage-based system

Kongsted, A. G., Nørgaard, J. V., Jensen, S. K., Lauridsen, C., Juul-Madsen, H. R., Norup, L. R., Engberg, R. M., Horsted, K. & Hermansen, J. E., 2015, In: Livestock Science. 178, p. 263-271

Bioassay-guided chromatographic isolation and identification of antibacterial compounds from Artemisia annua L. That inhibit Clostridium perfringens growth

Ivarsen, E., Fretté, X., Christensen, K. B., Christensen, L. P., Engberg, R. M., Grevsen, K. & Kjær, A., Oct 2014, In: A O A C International. Journal. 97, 5, p. 1282-1290 9 p.

Clostridium perfringens challenge and dietary fat type affect broiler chicken performance and fermentation in the gastrointestinal tract

Jozefiak, D., Kieronczyk, B., Rawski, M., Hejdysz, M., Rutkowski, A., Engberg, R. M. & Højberg, O., Jun 2014, In: Animal. 8, 6, p. 912-922 11 p.


BIOCONVAL: BIOCONVAL Integrated larvea production for feed in organic egg production

Engberg, R. M. & Jozefiak, D.


MultiChick: Diversity and integrity in organic poultry meat production (MultiChick)

Steenfeldt, S., Horsted, K., Noe, E. & Engberg, R. M.


Dysbakteriose hos slagtekyllinger

Engberg, R. M. & Ranjitkar, S.



Fodringsstrategi til at reducere verotoxin-producerende Escherichia coli hos kalve før slagtning

Engberg, R. M.

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Identification of potential biomarkers for leaky intestine in broiler chickens

Engberg, R. M., Naghizadeh, M. & Dalgaard, T. S.

24/03/2018 → …

MAFFRA: Multicomponent antibaterial feed additive for weaning piglets against intestinal diseases (MAFFRA)

Jensen, M., Canibe, N., Grevsen, K., Engberg, R. M., Hojberg, O., Lauridsen, C., Elgaard, T. & Williams, J.


Salmonelladiagnostik ved hjælp af luftprøver

Engberg, R. M.


Slagtekyllingers tarmsundhed, produktivitet og robusthed

Engberg, R. M.


Slagtekyllingers tarmsundhed, produktivitet og robusthed

Engberg, R. M.


SUMMER: Superb and Marketable Meat from Efficient and Robust Animals

Hermansen, J. E., Vestergaard, M., Juul-Madsen, H. R., Steenfeldt, S., Therkildsen, M., Jensen, S. K., Kongsted, A. G., Krystallis Krontalis, A., Engberg, R. M., Nørgaard, J. V., Lauridsen, C. & Zollitsch, W.

GUDP - Økologisk Forsknings-, Udviklings- og demonstrationsprogram


The efficacy of a carbohydrate based campylobacter vaccine against Campylobacter jejuni using a broiler challenge model

Engberg, R. M. & Naghizadeh, M.

13/02/2018 → …

UVIBA: UV kombilys i staldmiljø - virkning og effekt (UVIBA)

Canibe, N., Engberg, R. M., Jensen, S. K., Boes, J., Olesen, T., Svendsen, O. J., Jakobsen, M. L. & Olsen, J. E.