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Poul Nissen

On Allosteric Modulation of P-Type Cu+-ATPases

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  • Daniel Mattle, Denmark
  • Oleg Sitsel, Denmark
  • Henriette Elisabeth Autzen, Denmark
  • Gabriele Meloni, Denmark
  • Pontus Gourdon, Denmark
  • Poul Nissen
P-type ATPases perform active transport of various compounds across biological membranes and are crucial for ion homeostasis and the asymmetric composition of lipid bilayers. Although their functional cycle share principles of phosphoenzyme intermediates, P-type ATPases also show subclass-specific sequence motifs and structural elements that are linked to transport specificity and mechanistic modulation. Here we provide an overview of the Cu+-transporting ATPases (of subclass PIB) and compare them to the well-studied sarco(endo)plasmic reticulum Ca2 +-ATPase (of subclass PIIA). Cu+ ions in the cell are delivered by soluble chaperones to Cu+-ATPases, which expose a putative “docking platform” at the intracellular interface. Cu+-ATPases also contain heavy-metal binding domains providing a basis for allosteric control of pump activity. Database analysis of Cu+ ligating residues questions a two-site model of intramembranous Cu+ binding, and we suggest an alternative role for the proposed second site in copper translocation and proton exchange. The class-specific features demonstrate that topological diversity in P-type ATPases may tune a general energy coupling scheme to the translocation of compounds with remarkably different properties.
Original languageEnglish
JournalJournal of Molecular Biology
Pages (from-to)2299-2308
Number of pages9
Publication statusPublished - 10 Jul 2013

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