Poul Henning Jensen

Direct MRI-guided stereotaxic viral mediated gene transfer of alpha-synuclein in the Gottingen minipig CNS

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Direct MRI-guided stereotaxic viral mediated gene transfer of alpha-synuclein in the Gottingen minipig CNS. / GLUD, AN; Hedegaard, Claus; Nielsen, Mette Slot et al.

In: Acta Neurobiologiae Experimentalis, Vol. 71, No. 4, 2011, p. 508-518.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

GLUD, AN, Hedegaard, C, Nielsen, MS, Sorensen, JC, Bendixen, C, Jensen, PH, Mogensen, PH, Larsen, K & Bjarkam, CR 2011, 'Direct MRI-guided stereotaxic viral mediated gene transfer of alpha-synuclein in the Gottingen minipig CNS', Acta Neurobiologiae Experimentalis, vol. 71, no. 4, pp. 508-518.

APA

GLUD, AN., Hedegaard, C., Nielsen, M. S., Sorensen, J. C., Bendixen, C., Jensen, P. H., Mogensen, P. H., Larsen, K., & Bjarkam, C. R. (2011). Direct MRI-guided stereotaxic viral mediated gene transfer of alpha-synuclein in the Gottingen minipig CNS. Acta Neurobiologiae Experimentalis, 71(4), 508-518.

CBE

GLUD AN, Hedegaard C, Nielsen MS, Sorensen JC, Bendixen C, Jensen PH, Mogensen PH, Larsen K, Bjarkam CR. 2011. Direct MRI-guided stereotaxic viral mediated gene transfer of alpha-synuclein in the Gottingen minipig CNS. Acta Neurobiologiae Experimentalis. 71(4):508-518.

MLA

Vancouver

GLUD AN, Hedegaard C, Nielsen MS, Sorensen JC, Bendixen C, Jensen PH et al. Direct MRI-guided stereotaxic viral mediated gene transfer of alpha-synuclein in the Gottingen minipig CNS. Acta Neurobiologiae Experimentalis. 2011;71(4):508-518.

Author

GLUD, AN ; Hedegaard, Claus ; Nielsen, Mette Slot et al. / Direct MRI-guided stereotaxic viral mediated gene transfer of alpha-synuclein in the Gottingen minipig CNS. In: Acta Neurobiologiae Experimentalis. 2011 ; Vol. 71, No. 4. pp. 508-518.

Bibtex

@article{f475d72076154228bc4ba033348a6e57,
title = "Direct MRI-guided stereotaxic viral mediated gene transfer of alpha-synuclein in the Gottingen minipig CNS",
abstract = "The aim was to establish a non-primate large animal PD model by lentiviral vector mediated mutant alpha-synuclein overexpression in the substantia nigra. Lentivirus encoding A53T alpha-synuclein (6 x 2.5 microliter) was stereotaxically injected into the substantia nigra of six adult female Gottingen minipigs. Contralateral control injections encoding enhanced green fluorescent protein (EGFP) were performed. Gait-analysis was performed pre- and postoperatively. PCR of the transgenes and immunohistochemical staining against alpha-synuclein, EGFP, GFAP and TH was performed after 20 weeks. Gait analysis revealed a significant increase in step length and height, and a decrease in the double stand phase. PCR verified the mesencephalic presence of transgenes. IHC analysis showed alpha-synuclein expression in nigral neurons, around the injection tract and in related nigrostriatal projections. The alpha-synuclein positive neurons appeared swollen and vacuolated, in contrast to the EGFP-injected control side. To transduct all nigrostriatal cells with few microinjections, wider dissemination of the transgene must be achieved.",
author = "AN GLUD and Claus Hedegaard and Nielsen, {Mette Slot} and Sorensen, {Jens Christian} and Christian Bendixen and Jensen, {Poul Henning} and Mogensen, {Poul Henning} and Knud Larsen and Bjarkam, {Carsten Reidies}",
year = "2011",
language = "English",
volume = "71",
pages = "508--518",
journal = "Acta Neurobiologiae Experimentalis",
issn = "0065-1400",
publisher = "Polska Akademia Nauk Instytut Biologii Doswiadczalnej im. M. Nenckiego",
number = "4",

}

RIS

TY - JOUR

T1 - Direct MRI-guided stereotaxic viral mediated gene transfer of alpha-synuclein in the Gottingen minipig CNS

AU - GLUD, AN

AU - Hedegaard, Claus

AU - Nielsen, Mette Slot

AU - Sorensen, Jens Christian

AU - Bendixen, Christian

AU - Jensen, Poul Henning

AU - Mogensen, Poul Henning

AU - Larsen, Knud

AU - Bjarkam, Carsten Reidies

PY - 2011

Y1 - 2011

N2 - The aim was to establish a non-primate large animal PD model by lentiviral vector mediated mutant alpha-synuclein overexpression in the substantia nigra. Lentivirus encoding A53T alpha-synuclein (6 x 2.5 microliter) was stereotaxically injected into the substantia nigra of six adult female Gottingen minipigs. Contralateral control injections encoding enhanced green fluorescent protein (EGFP) were performed. Gait-analysis was performed pre- and postoperatively. PCR of the transgenes and immunohistochemical staining against alpha-synuclein, EGFP, GFAP and TH was performed after 20 weeks. Gait analysis revealed a significant increase in step length and height, and a decrease in the double stand phase. PCR verified the mesencephalic presence of transgenes. IHC analysis showed alpha-synuclein expression in nigral neurons, around the injection tract and in related nigrostriatal projections. The alpha-synuclein positive neurons appeared swollen and vacuolated, in contrast to the EGFP-injected control side. To transduct all nigrostriatal cells with few microinjections, wider dissemination of the transgene must be achieved.

AB - The aim was to establish a non-primate large animal PD model by lentiviral vector mediated mutant alpha-synuclein overexpression in the substantia nigra. Lentivirus encoding A53T alpha-synuclein (6 x 2.5 microliter) was stereotaxically injected into the substantia nigra of six adult female Gottingen minipigs. Contralateral control injections encoding enhanced green fluorescent protein (EGFP) were performed. Gait-analysis was performed pre- and postoperatively. PCR of the transgenes and immunohistochemical staining against alpha-synuclein, EGFP, GFAP and TH was performed after 20 weeks. Gait analysis revealed a significant increase in step length and height, and a decrease in the double stand phase. PCR verified the mesencephalic presence of transgenes. IHC analysis showed alpha-synuclein expression in nigral neurons, around the injection tract and in related nigrostriatal projections. The alpha-synuclein positive neurons appeared swollen and vacuolated, in contrast to the EGFP-injected control side. To transduct all nigrostriatal cells with few microinjections, wider dissemination of the transgene must be achieved.

M3 - Journal article

C2 - 22237496

VL - 71

SP - 508

EP - 518

JO - Acta Neurobiologiae Experimentalis

JF - Acta Neurobiologiae Experimentalis

SN - 0065-1400

IS - 4

ER -