Poul Henning Jensen

Direct gene transfer in the Gottingen minipig CNS using stereotaxic lentiviral microinjections

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Department of Medical Biochemistry
  • Molekylær Genetik og Systembiologi
  • Master Program in Biomedical Engineering
  • Department of Anatomy
We aim to induce direct viral mediated gene transfer in the substantia nigra (SN) of the Gottingen minipig using MRI guided stereotaxic injections of lentiviral vectors encoding enhanced green fluorescent protein (EGFP). Nine female Gottingen minipigs were injected unilaterally into the SN with 6 per 2.5 microliters lentivirus capable of transducing cells and mediating expression of recombinant EGFP. The animals were euthanized after four (n=3) or twenty weeks (n=6). Fresh brain tissue from three animals was used for PCR. The remaining six brains were cryo- or paraffin-sectioned for fluorescence, Nissl-, and immunohistochemical EGFP visualization. EGFP was seen in nigral neurons, axons, glial cells, endothelial cells, and in nigral fibers targeting the striatum. PCR-based detection confirmed the presence of the transgene in SN, whereas all other examined brain areas were negative, indicating that the immunohistochemically detected EGFP in the striatum derived from transfected nigral cells.
Original languageEnglish
JournalActa Neurobiologiae Experimentalis
Volume70
Issue3
Pages (from-to)308-315
Number of pages8
ISSN0065-1400
Publication statusPublished - 2010

    Research areas

  • animal model, gene therapy, substantia nigra, Sus scrofa, transgenesis

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