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Peter Kappel Theil

Oxidative stress-induced metabolic changes in mouse C2C12 myotubes studied with high-resolution 13C, 1H, and 31P NMR spectroscopy

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Oxidative stress-induced metabolic changes in mouse C2C12 myotubes studied with high-resolution 13C, 1H, and 31P NMR spectroscopy. / Straadt, Ida K; Young, Jette F; Petersen, Bent O; Duus, Jens Ø; Gregersen, Niels; Bross, Peter; Oksbjerg, Niels; Theil, Peter K; Bertram, Hanne C.

In: Journal of Agricultural and Food Chemistry, Vol. 58, No. 3, 2010, p. 1918-26.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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Author

Straadt, Ida K ; Young, Jette F ; Petersen, Bent O ; Duus, Jens Ø ; Gregersen, Niels ; Bross, Peter ; Oksbjerg, Niels ; Theil, Peter K ; Bertram, Hanne C. / Oxidative stress-induced metabolic changes in mouse C2C12 myotubes studied with high-resolution 13C, 1H, and 31P NMR spectroscopy. In: Journal of Agricultural and Food Chemistry. 2010 ; Vol. 58, No. 3. pp. 1918-26.

Bibtex

@article{89d22700c54511de8cbb000ea68e967b,
title = "Oxidative stress-induced metabolic changes in mouse C2C12 myotubes studied with high-resolution 13C, 1H, and 31P NMR spectroscopy",
abstract = "In this study, stress in relation to slaughter was investigated in a model system by the use of (13)C, (1)H, and (31)P nuclear magnetic resonance (NMR) spectroscopy for elucidating changes in the metabolites in C2C12 myotubes exposed to H(2)O(2)-induced stress. Oxidative stress resulted in lower levels of several metabolites, mainly amino acids; however, higher levels of alanine were apparent in the (13)C spectra after incubation with [(13)C(1)]glucose. In the (13)C spectra [(13)C(3)]lactate tended to increase after exposure to increasing concentrations of H(2)O(2); conversely, a tendency to lower levels of the unlabeled ((12)C) lactate were identified in the (1)H spectra after stress exposure. These data indicate an increase in de novo synthesis of alanine, concomitant with a release of lactate from the myotubes to the medium at oxidative stress conditions. The changes in the metabolite levels could possibly be useful as markers for meat quality traits.",
author = "Straadt, {Ida K} and Young, {Jette F} and Petersen, {Bent O} and Duus, {Jens {\O}} and Niels Gregersen and Peter Bross and Niels Oksbjerg and Theil, {Peter K} and Bertram, {Hanne C}",
year = "2010",
doi = "10.1021/jf903505a",
language = "English",
volume = "58",
pages = "1918--26",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "AMER CHEMICAL SOC",
number = "3",

}

RIS

TY - JOUR

T1 - Oxidative stress-induced metabolic changes in mouse C2C12 myotubes studied with high-resolution 13C, 1H, and 31P NMR spectroscopy

AU - Straadt, Ida K

AU - Young, Jette F

AU - Petersen, Bent O

AU - Duus, Jens Ø

AU - Gregersen, Niels

AU - Bross, Peter

AU - Oksbjerg, Niels

AU - Theil, Peter K

AU - Bertram, Hanne C

PY - 2010

Y1 - 2010

N2 - In this study, stress in relation to slaughter was investigated in a model system by the use of (13)C, (1)H, and (31)P nuclear magnetic resonance (NMR) spectroscopy for elucidating changes in the metabolites in C2C12 myotubes exposed to H(2)O(2)-induced stress. Oxidative stress resulted in lower levels of several metabolites, mainly amino acids; however, higher levels of alanine were apparent in the (13)C spectra after incubation with [(13)C(1)]glucose. In the (13)C spectra [(13)C(3)]lactate tended to increase after exposure to increasing concentrations of H(2)O(2); conversely, a tendency to lower levels of the unlabeled ((12)C) lactate were identified in the (1)H spectra after stress exposure. These data indicate an increase in de novo synthesis of alanine, concomitant with a release of lactate from the myotubes to the medium at oxidative stress conditions. The changes in the metabolite levels could possibly be useful as markers for meat quality traits.

AB - In this study, stress in relation to slaughter was investigated in a model system by the use of (13)C, (1)H, and (31)P nuclear magnetic resonance (NMR) spectroscopy for elucidating changes in the metabolites in C2C12 myotubes exposed to H(2)O(2)-induced stress. Oxidative stress resulted in lower levels of several metabolites, mainly amino acids; however, higher levels of alanine were apparent in the (13)C spectra after incubation with [(13)C(1)]glucose. In the (13)C spectra [(13)C(3)]lactate tended to increase after exposure to increasing concentrations of H(2)O(2); conversely, a tendency to lower levels of the unlabeled ((12)C) lactate were identified in the (1)H spectra after stress exposure. These data indicate an increase in de novo synthesis of alanine, concomitant with a release of lactate from the myotubes to the medium at oxidative stress conditions. The changes in the metabolite levels could possibly be useful as markers for meat quality traits.

U2 - 10.1021/jf903505a

DO - 10.1021/jf903505a

M3 - Journal article

C2 - 20073468

VL - 58

SP - 1918

EP - 1926

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 3

ER -