Aarhus University Seal / Aarhus Universitets segl

Peter Kappel Theil

Oxidative stress-induced metabolic changes in mouse C2C12 myotubes studied with high-resolution 13C, 1H, and 31P NMR spectroscopy

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Nutrition and Production Physiology
  • Dairy, Meat and Egg Quality and Technology
  • Plant Food Science
  • Department of Food Science
  • Department of Animal Health and Bioscience
  • Research Unit for Molecular Medicine
In this study, stress in relation to slaughter was investigated in a model system by the use of (13)C, (1)H, and (31)P nuclear magnetic resonance (NMR) spectroscopy for elucidating changes in the metabolites in C2C12 myotubes exposed to H(2)O(2)-induced stress. Oxidative stress resulted in lower levels of several metabolites, mainly amino acids; however, higher levels of alanine were apparent in the (13)C spectra after incubation with [(13)C(1)]glucose. In the (13)C spectra [(13)C(3)]lactate tended to increase after exposure to increasing concentrations of H(2)O(2); conversely, a tendency to lower levels of the unlabeled ((12)C) lactate were identified in the (1)H spectra after stress exposure. These data indicate an increase in de novo synthesis of alanine, concomitant with a release of lactate from the myotubes to the medium at oxidative stress conditions. The changes in the metabolite levels could possibly be useful as markers for meat quality traits.
Original languageEnglish
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue3
Pages (from-to)1918-26
Number of pages9
ISSN0021-8561
DOIs
Publication statusPublished - 2010

See relations at Aarhus University Citationformats

ID: 2785648