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Peter Kappel Theil

Hepatic metabolism of anaesthetized growing pigs during acute portal infusion of volatile fatty acids and hydroxy-methyl butyrate

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Hepatic metabolism of anaesthetized growing pigs during acute portal infusion of volatile fatty acids and hydroxy-methyl butyrate. / Theil, Peter Kappel; Larsen, Uffe Krogh; Bjerre-Harpøth, Vibeke; Storm, Adam Christian.

In: Journal of Animal Science, Vol. 94, No. Suppl. 3, 09.11.2016, p. 324-327.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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Theil, PK, Larsen, UK, Bjerre-Harpøth, V & Storm, AC 2016, 'Hepatic metabolism of anaesthetized growing pigs during acute portal infusion of volatile fatty acids and hydroxy-methyl butyrate', Journal of Animal Science, vol. 94, no. Suppl. 3, pp. 324-327. https://doi.org/10.2527/jas.2015-9780

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Author

Theil, Peter Kappel ; Larsen, Uffe Krogh ; Bjerre-Harpøth, Vibeke ; Storm, Adam Christian. / Hepatic metabolism of anaesthetized growing pigs during acute portal infusion of volatile fatty acids and hydroxy-methyl butyrate. In: Journal of Animal Science. 2016 ; Vol. 94, No. Suppl. 3. pp. 324-327.

Bibtex

@article{1f24a2f50a134431862db7aeaf9257ed,
title = "Hepatic metabolism of anaesthetized growing pigs during acute portal infusion of volatile fatty acids and hydroxy-methyl butyrate",
abstract = "ABSTRACT: The objective of the experiment was to study hepatic metabolism during infusion of volatile fatty acids (VFA) differing in amounts and composition or infusion of HMB. Three fasted (20 h) pigs (mean BW ± SE; 58 kg ± 1) were fitted with indwelling catheters in the portal vein, hepatic vein, mesenteric artery and two in mesenteric veins. One of the mesenteric vein catheters was used to infuse VFA in the anesthetized pigs to mimic effects of increased consumption of dietary fibers. Sixteen sets of blood samples were simultaneously drawn from the artery and portal and hepatic veins at 15 min intervals and analyzed for contents of paraamino- hippuric acid (PAH; blood flow marker) and plasma metabolites. Total VFA was infused at a rate of 0 mmol/h (background; Inf1, Inf6), 60 mmol/h (Inf2) or 120 mmol/h (Inf3 to Inf5). Infused VFA contained 70, 20, and 5% of acetate, propionate, and butyrate, respectively, for Inf2 and Inf3, or 65%, 20%, and 10% of acetate, propionate, and butyrate, respectively, for Inf4 and Inf5. In addition, for Inf5, HMB was infused at 2 mmol/h. Statistical analysis included fixed effects of infusion and interaction between infusion and samplings within infusion while accounting for repeated measurements. A net hepatic uptake of propionate, butyrate, and lactate was observed, whereas the liver released acetate, glucose, and urea. The portal lactate absorption could not account for the net hepatic uptake of lactate, suggesting lactate originated from partial oxidation of glycogen in peripheral muscle tissues. The net hepatic lactate uptake could account for 29% to 84% of the hepatic glucose released during VFA infusions. Net portal recovery rates of infused VFA were between 90% and 105%, indicating that PAH is a reliable blood flow marker. In conclusion, lactate and AA from peripheral tissues were likely the two most important glycogenic precursors for gluconeogenesis in the liver during fasting.",
keywords = "dietary fiber, fermentation, liver, plasma flow, quantitative metabolism",
author = "Theil, {Peter Kappel} and Larsen, {Uffe Krogh} and Vibeke Bjerre-Harp{\o}th and Storm, {Adam Christian}",
year = "2016",
month = nov,
day = "9",
doi = "10.2527/jas.2015-9780",
language = "English",
volume = "94",
pages = "324--327",
journal = "Journal of Animal Science",
issn = "0021-8812",
publisher = "AMER SOC ANIMAL SCIENCE",
number = "Suppl. 3",

}

RIS

TY - JOUR

T1 - Hepatic metabolism of anaesthetized growing pigs during acute portal infusion of volatile fatty acids and hydroxy-methyl butyrate

AU - Theil, Peter Kappel

AU - Larsen, Uffe Krogh

AU - Bjerre-Harpøth, Vibeke

AU - Storm, Adam Christian

PY - 2016/11/9

Y1 - 2016/11/9

N2 - ABSTRACT: The objective of the experiment was to study hepatic metabolism during infusion of volatile fatty acids (VFA) differing in amounts and composition or infusion of HMB. Three fasted (20 h) pigs (mean BW ± SE; 58 kg ± 1) were fitted with indwelling catheters in the portal vein, hepatic vein, mesenteric artery and two in mesenteric veins. One of the mesenteric vein catheters was used to infuse VFA in the anesthetized pigs to mimic effects of increased consumption of dietary fibers. Sixteen sets of blood samples were simultaneously drawn from the artery and portal and hepatic veins at 15 min intervals and analyzed for contents of paraamino- hippuric acid (PAH; blood flow marker) and plasma metabolites. Total VFA was infused at a rate of 0 mmol/h (background; Inf1, Inf6), 60 mmol/h (Inf2) or 120 mmol/h (Inf3 to Inf5). Infused VFA contained 70, 20, and 5% of acetate, propionate, and butyrate, respectively, for Inf2 and Inf3, or 65%, 20%, and 10% of acetate, propionate, and butyrate, respectively, for Inf4 and Inf5. In addition, for Inf5, HMB was infused at 2 mmol/h. Statistical analysis included fixed effects of infusion and interaction between infusion and samplings within infusion while accounting for repeated measurements. A net hepatic uptake of propionate, butyrate, and lactate was observed, whereas the liver released acetate, glucose, and urea. The portal lactate absorption could not account for the net hepatic uptake of lactate, suggesting lactate originated from partial oxidation of glycogen in peripheral muscle tissues. The net hepatic lactate uptake could account for 29% to 84% of the hepatic glucose released during VFA infusions. Net portal recovery rates of infused VFA were between 90% and 105%, indicating that PAH is a reliable blood flow marker. In conclusion, lactate and AA from peripheral tissues were likely the two most important glycogenic precursors for gluconeogenesis in the liver during fasting.

AB - ABSTRACT: The objective of the experiment was to study hepatic metabolism during infusion of volatile fatty acids (VFA) differing in amounts and composition or infusion of HMB. Three fasted (20 h) pigs (mean BW ± SE; 58 kg ± 1) were fitted with indwelling catheters in the portal vein, hepatic vein, mesenteric artery and two in mesenteric veins. One of the mesenteric vein catheters was used to infuse VFA in the anesthetized pigs to mimic effects of increased consumption of dietary fibers. Sixteen sets of blood samples were simultaneously drawn from the artery and portal and hepatic veins at 15 min intervals and analyzed for contents of paraamino- hippuric acid (PAH; blood flow marker) and plasma metabolites. Total VFA was infused at a rate of 0 mmol/h (background; Inf1, Inf6), 60 mmol/h (Inf2) or 120 mmol/h (Inf3 to Inf5). Infused VFA contained 70, 20, and 5% of acetate, propionate, and butyrate, respectively, for Inf2 and Inf3, or 65%, 20%, and 10% of acetate, propionate, and butyrate, respectively, for Inf4 and Inf5. In addition, for Inf5, HMB was infused at 2 mmol/h. Statistical analysis included fixed effects of infusion and interaction between infusion and samplings within infusion while accounting for repeated measurements. A net hepatic uptake of propionate, butyrate, and lactate was observed, whereas the liver released acetate, glucose, and urea. The portal lactate absorption could not account for the net hepatic uptake of lactate, suggesting lactate originated from partial oxidation of glycogen in peripheral muscle tissues. The net hepatic lactate uptake could account for 29% to 84% of the hepatic glucose released during VFA infusions. Net portal recovery rates of infused VFA were between 90% and 105%, indicating that PAH is a reliable blood flow marker. In conclusion, lactate and AA from peripheral tissues were likely the two most important glycogenic precursors for gluconeogenesis in the liver during fasting.

KW - dietary fiber

KW - fermentation

KW - liver

KW - plasma flow

KW - quantitative metabolism

U2 - 10.2527/jas.2015-9780

DO - 10.2527/jas.2015-9780

M3 - Journal article

VL - 94

SP - 324

EP - 327

JO - Journal of Animal Science

JF - Journal of Animal Science

SN - 0021-8812

IS - Suppl. 3

ER -