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Peter Kappel Theil

Comparison of urine production measured by total collection and para-aminohippuric acid infusion methods

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  • Takele Feyera
  • Pan Zhou, A Key Laboratory of Animal Disease-Resistance Nutrition and Feed Science, Ministry of Agriculture, P. R. China, Animal Nutrition Institute, Sichuan Agricultural University
  • ,
  • Trine Friis Pedersen
  • ,
  • Uffe Krogh Larsen
  • ,
  • Peter Kappel Theil
Urine production measured by para-aminohippuric acid (pAH) infusion method is more accurate than total collection method but it need surgical implantation of catheter and pAH pump. The present study aimed to compare sow urine production measured by total collection (TC) for six hours with that measured by para-aminohippuric acid (pAH) infusion. Ten sows were surgically implanted with mammary vein catheter for infusion of pAH on 0 (during farrowing), 7 and 17 day in milk (DIM). Insertion of urinary catheter and infusion of pAH was done at least 2 hours before the first sampling and the bladder was emptied at 0, 2, 4 and 6 hours on 0, 7 and 17 DIM. The collected urine at 2, 4 and 6 hours were weighed and subsampled for pAH analysis. Urine production measured by pAH method was calculated as infusion rate of pAH/concentration of pAH in the urine. Urine measured by the TC and pAH methods were analyzed using the MIXED procedure of SAS including sampling time, DIM and their interaction as a fixed effect and sow as a random component. Correlation of urine measured by the two methods was assessed using the CORR procedure of SAS. The results were reported as mean±SEM. Average urine production measured by the TC and pAH methods were 163±19 and 170±17 g/h, respectively, and these two methods were highly correlated (r = 0.84; P < 0.001). Irrespective of the methods, urine production was decreased as sampling time progressed (P=0.002), but was similar across DIM. The linear regression of TC method on pAH method had an intercept which was close to zero (-0.30±139; P=0.99) and slope close to one (0.96±0.12; P<0.001). In conclusion, urine production measured by the pAH method and TC for 6 hours gives comparable results and neither of the two methods seems to be superior in terms of precision.
Original languageEnglish
Title of host publicationProceedings of the 14th International Symposium on Digestive Physiology of Pigs
Publication year21 Aug 2018
Article number505
Publication statusPublished - 21 Aug 2018
Event14th International Symposium on Digestive Physiology of Pigs - Brisbane, Australia
Duration: 21 Aug 201824 Aug 2018
Conference number: 14

Conference

Conference14th International Symposium on Digestive Physiology of Pigs
Nummer14
LandAustralia
ByBrisbane
Periode21/08/201824/08/2018

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