Peter Funch

Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis. / Eriksen, Niels Thomas; Wollenberg, Rasmus Dam ; Tophøj, Jakob; Sondergaard, Teis Esben; Funch, Peter; Andersen, Per.

In: Scientific Reports, Vol. 9, No. 1, 14543, 10.10.2019.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Eriksen, NT, Wollenberg, RD, Tophøj, J, Sondergaard, TE, Funch, P & Andersen, P 2019, 'Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis', Scientific Reports, vol. 9, no. 1, 14543. https://doi.org/10.1038/s41598-019-50998-0

APA

Eriksen, N. T., Wollenberg, R. D., Tophøj, J., Sondergaard, T. E., Funch, P., & Andersen, P. (2019). Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis. Scientific Reports, 9(1), [14543]. https://doi.org/10.1038/s41598-019-50998-0

CBE

Eriksen NT, Wollenberg RD, Tophøj J, Sondergaard TE, Funch P, Andersen P. 2019. Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis. Scientific Reports. 9(1):Article 14543. https://doi.org/10.1038/s41598-019-50998-0

MLA

Vancouver

Eriksen NT, Wollenberg RD, Tophøj J, Sondergaard TE, Funch P, Andersen P. Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis. Scientific Reports. 2019 Oct 10;9(1). 14543. https://doi.org/10.1038/s41598-019-50998-0

Author

Eriksen, Niels Thomas ; Wollenberg, Rasmus Dam ; Tophøj, Jakob ; Sondergaard, Teis Esben ; Funch, Peter ; Andersen, Per. / Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis. In: Scientific Reports. 2019 ; Vol. 9, No. 1.

Bibtex

@article{4ca0d5f5a6164372a68c8b6522fdb47c,
title = "Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis",
abstract = "Choanoflagellates are common members of planktonic communities. Some have complex life histories that involve transitions between multiple cell stages. We have grown the loricate choanoflagellate Diaphanoeca grandis on the bacterium Pantoea sp. and integrated kinetic observations at the culture level and at the single cell level. The life history of D. grandis includes a cell division cycle with a number of recognisable cell stages. Mature, loricate D. grandis were immobile and settled on the bottom substratum. Daughter cells were ejected from the lorica 30 min. after cell division, became motile and glided on the bottom substratum until they assembled a lorica. Single cell kinetics could explain overall growth kinetics in D. grandis cultures. The specific growth rate was 0.72 day−1 during exponential growth while mature D. grandis produced daughter cells at a rate of 0.9 day−1. Daughter cells took about 1.2 h to mature. D. grandis was able to abandon and replace its lorica, an event thatdelayed daughter cell formation by more than 2 days. The frequency of daughter cell formation varied considerably among individuals and single cell kinetics demonstrated an extensive degree of heterogeneity in D. grandis cultures, also when growth appeared to be balanced.",
author = "Eriksen, {Niels Thomas} and Wollenberg, {Rasmus Dam} and Jakob Toph{\o}j and Sondergaard, {Teis Esben} and Peter Funch and Per Andersen",
year = "2019",
month = oct,
day = "10",
doi = "10.1038/s41598-019-50998-0",
language = "English",
volume = "9",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis

AU - Eriksen, Niels Thomas

AU - Wollenberg, Rasmus Dam

AU - Tophøj, Jakob

AU - Sondergaard, Teis Esben

AU - Funch, Peter

AU - Andersen, Per

PY - 2019/10/10

Y1 - 2019/10/10

N2 - Choanoflagellates are common members of planktonic communities. Some have complex life histories that involve transitions between multiple cell stages. We have grown the loricate choanoflagellate Diaphanoeca grandis on the bacterium Pantoea sp. and integrated kinetic observations at the culture level and at the single cell level. The life history of D. grandis includes a cell division cycle with a number of recognisable cell stages. Mature, loricate D. grandis were immobile and settled on the bottom substratum. Daughter cells were ejected from the lorica 30 min. after cell division, became motile and glided on the bottom substratum until they assembled a lorica. Single cell kinetics could explain overall growth kinetics in D. grandis cultures. The specific growth rate was 0.72 day−1 during exponential growth while mature D. grandis produced daughter cells at a rate of 0.9 day−1. Daughter cells took about 1.2 h to mature. D. grandis was able to abandon and replace its lorica, an event thatdelayed daughter cell formation by more than 2 days. The frequency of daughter cell formation varied considerably among individuals and single cell kinetics demonstrated an extensive degree of heterogeneity in D. grandis cultures, also when growth appeared to be balanced.

AB - Choanoflagellates are common members of planktonic communities. Some have complex life histories that involve transitions between multiple cell stages. We have grown the loricate choanoflagellate Diaphanoeca grandis on the bacterium Pantoea sp. and integrated kinetic observations at the culture level and at the single cell level. The life history of D. grandis includes a cell division cycle with a number of recognisable cell stages. Mature, loricate D. grandis were immobile and settled on the bottom substratum. Daughter cells were ejected from the lorica 30 min. after cell division, became motile and glided on the bottom substratum until they assembled a lorica. Single cell kinetics could explain overall growth kinetics in D. grandis cultures. The specific growth rate was 0.72 day−1 during exponential growth while mature D. grandis produced daughter cells at a rate of 0.9 day−1. Daughter cells took about 1.2 h to mature. D. grandis was able to abandon and replace its lorica, an event thatdelayed daughter cell formation by more than 2 days. The frequency of daughter cell formation varied considerably among individuals and single cell kinetics demonstrated an extensive degree of heterogeneity in D. grandis cultures, also when growth appeared to be balanced.

U2 - 10.1038/s41598-019-50998-0

DO - 10.1038/s41598-019-50998-0

M3 - Journal article

C2 - 31601859

VL - 9

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 14543

ER -