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Peter Funch

Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis

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  • Niels Thomas Eriksen, Aalborg Universitet
  • ,
  • Rasmus Dam Wollenberg, Aalborg University, Denmark
  • Jakob Tophøj, Aalborg Universitet
  • ,
  • Teis Esben Sondergaard, Aalborg University, Denmark
  • Peter Funch
  • Per Andersen
Choanoflagellates are common members of planktonic communities. Some have complex life histories that involve transitions between multiple cell stages. We have grown the loricate choanoflagellate Diaphanoeca grandis on the bacterium Pantoea sp. and integrated kinetic observations at the culture level and at the single cell level. The life history of D. grandis includes a cell division cycle with a number of recognisable cell stages. Mature, loricate D. grandis were immobile and settled on the bottom substratum. Daughter cells were ejected from the lorica 30 min. after cell division, became motile and glided on the bottom substratum until they assembled a lorica. Single cell kinetics could explain overall growth kinetics in D. grandis cultures. The specific growth rate was 0.72 day−1 during exponential growth while mature D. grandis produced daughter cells at a rate of 0.9 day−1. Daughter cells took about 1.2 h to mature. D. grandis was able to abandon and replace its lorica, an event that
delayed daughter cell formation by more than 2 days. The frequency of daughter cell formation varied considerably among individuals and single cell kinetics demonstrated an extensive degree of heterogeneity in D. grandis cultures, also when growth appeared to be balanced.
Original languageEnglish
Article number14543
JournalScientific Reports
Volume9
Issue1
Number of pages1
ISSN2045-2322
DOIs
Publication statusPublished - 10 Oct 2019

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