Ole Bækgaard Nielsen

Extracellular magnesium and calcium reduce myotonia in isolated ClC-1 chloride channel-inhibited human muscle

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Extracellular magnesium and calcium reduce myotonia in isolated ClC-1 chloride channel-inhibited human muscle. / Skov, Martin; De Paoli, Frank Vincenzo; Lausten, Jesper; Nielsen, Ole Baekgaard; Pedersen, Thomas Holm.

In: Muscle & Nerve, Vol. 51, No. 1, 01.2015, p. 65-71.

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@article{f642e80fc9c04b8297291bf904bcbc82,
title = "Extracellular magnesium and calcium reduce myotonia in isolated ClC-1 chloride channel-inhibited human muscle",
abstract = "INTRODUCTION: Experimental myotonia induced in rat muscle by ClC-1 chloride channel-inhibited has been shown to be related inversely to extracellular concentrations of Mg(2+) and Ca(2+) ([Mg(2+) ]o and [Ca(2+) ]o) within physiological ranges. Because this implicates a role for [Mg(2+)]o and [Ca(2+)]o in the variability of symptoms among myotonia congenita patients, we searched for similar effects of [Mg(2+)]o and [Ca(2+)]o on myotonia in human muscle.METHODS: Bundles of muscle fibers were isolated from abdominal rectus in patients undergoing abdominal surgery. Myotonia was induced by ClC-1 inhibition using 9-anthracene carboxylic acid (9-AC) and was assessed from integrals of force induced by 5-Hz stimulation for 2 seconds.RESULTS: Myotonia disappeared gradually when [Mg(2+)]o or [Ca(2+)]o were elevated throughout their physiological ranges. These effects of [Mg(2+)]o and [Ca(2+)]o were additive and interchangeable.CONCLUSIONS: These findings suggest that variations in symptoms in myotonia congenita patients may arise from physiological variations in serum Mg(2+) and Ca(2+).",
keywords = "Adult, Aged, Aged, 80 and over, Anthracenes, Area Under Curve, Biophysics, Calcium, Chloride Channels, Dose-Response Relationship, Drug, Electric Stimulation, Female, Humans, In Vitro Techniques, Magnesium, Male, Middle Aged, Muscle Contraction, Muscle Fibers, Skeletal, Myotonia",
author = "Martin Skov and {De Paoli}, {Frank Vincenzo} and Jesper Lausten and Nielsen, {Ole Baekgaard} and Pedersen, {Thomas Holm}",
note = "{\textcopyright} 2014 Wiley Periodicals, Inc.",
year = "2015",
month = jan,
doi = "10.1002/mus.24260",
language = "English",
volume = "51",
pages = "65--71",
journal = "Muscle & Nerve",
issn = "0148-639X",
publisher = "JohnWiley & Sons, Inc.",
number = "1",

}

RIS

TY - JOUR

T1 - Extracellular magnesium and calcium reduce myotonia in isolated ClC-1 chloride channel-inhibited human muscle

AU - Skov, Martin

AU - De Paoli, Frank Vincenzo

AU - Lausten, Jesper

AU - Nielsen, Ole Baekgaard

AU - Pedersen, Thomas Holm

N1 - © 2014 Wiley Periodicals, Inc.

PY - 2015/1

Y1 - 2015/1

N2 - INTRODUCTION: Experimental myotonia induced in rat muscle by ClC-1 chloride channel-inhibited has been shown to be related inversely to extracellular concentrations of Mg(2+) and Ca(2+) ([Mg(2+) ]o and [Ca(2+) ]o) within physiological ranges. Because this implicates a role for [Mg(2+)]o and [Ca(2+)]o in the variability of symptoms among myotonia congenita patients, we searched for similar effects of [Mg(2+)]o and [Ca(2+)]o on myotonia in human muscle.METHODS: Bundles of muscle fibers were isolated from abdominal rectus in patients undergoing abdominal surgery. Myotonia was induced by ClC-1 inhibition using 9-anthracene carboxylic acid (9-AC) and was assessed from integrals of force induced by 5-Hz stimulation for 2 seconds.RESULTS: Myotonia disappeared gradually when [Mg(2+)]o or [Ca(2+)]o were elevated throughout their physiological ranges. These effects of [Mg(2+)]o and [Ca(2+)]o were additive and interchangeable.CONCLUSIONS: These findings suggest that variations in symptoms in myotonia congenita patients may arise from physiological variations in serum Mg(2+) and Ca(2+).

AB - INTRODUCTION: Experimental myotonia induced in rat muscle by ClC-1 chloride channel-inhibited has been shown to be related inversely to extracellular concentrations of Mg(2+) and Ca(2+) ([Mg(2+) ]o and [Ca(2+) ]o) within physiological ranges. Because this implicates a role for [Mg(2+)]o and [Ca(2+)]o in the variability of symptoms among myotonia congenita patients, we searched for similar effects of [Mg(2+)]o and [Ca(2+)]o on myotonia in human muscle.METHODS: Bundles of muscle fibers were isolated from abdominal rectus in patients undergoing abdominal surgery. Myotonia was induced by ClC-1 inhibition using 9-anthracene carboxylic acid (9-AC) and was assessed from integrals of force induced by 5-Hz stimulation for 2 seconds.RESULTS: Myotonia disappeared gradually when [Mg(2+)]o or [Ca(2+)]o were elevated throughout their physiological ranges. These effects of [Mg(2+)]o and [Ca(2+)]o were additive and interchangeable.CONCLUSIONS: These findings suggest that variations in symptoms in myotonia congenita patients may arise from physiological variations in serum Mg(2+) and Ca(2+).

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Anthracenes

KW - Area Under Curve

KW - Biophysics

KW - Calcium

KW - Chloride Channels

KW - Dose-Response Relationship, Drug

KW - Electric Stimulation

KW - Female

KW - Humans

KW - In Vitro Techniques

KW - Magnesium

KW - Male

KW - Middle Aged

KW - Muscle Contraction

KW - Muscle Fibers, Skeletal

KW - Myotonia

U2 - 10.1002/mus.24260

DO - 10.1002/mus.24260

M3 - Journal article

C2 - 24710922

VL - 51

SP - 65

EP - 71

JO - Muscle & Nerve

JF - Muscle & Nerve

SN - 0148-639X

IS - 1

ER -