Aarhus University Seal / Aarhus Universitets segl

Mogens Agerbo Krogh

Evaluation of Two Fecal Occult Blood Tests for Detecting Non-Perforating Abomasal Lesions in Cattle

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review


  • Sarah Lee Munch, University of Copenhagen, Denmark
  • Søren Saxmose Nielsen, University of Copenhagen, Denmark
  • Mogens Agerbo Krogh
  • Nynne Capion, University of Copenhagen, Denmark
Abomasal lesions in dairy cattle are highly prevalent, but diagnosis of the non-penetrating lesions is a challenge. We performed one experiment to estimate the amount of blood detectable in feces using two different tests, one experiment to determine if hemoglobin is degraded in the rumen to find possible false-positive tests due to blood from extra gastrointestinal sources and subsequently an observational study to estimate the diagnostic properties of the test with the observed lower detection limit. The observational study included primarily dairy cattle at slaughter, where we could observe the actual lesions postmortem. The detection limits of the tests marketed as Hemo-Fec® and Hemoccult II® SENSA® were 1–2 mL blood/kg feces and 2–4.5 mL blood/kg feces, respectively. Hemoglobin was not degraded in ruminal fluid and could possibly bypass the rumen and be detected in feces. In the observational study, the Hemo-Fec® test had no diagnostic value in dairy cattle with superficial erosions, with scarring, and with <4 acute or chronic lesions. The test had diagnostic potential in cattle with ≥4 acute or chronic lesions, where the proportion of true positives exceeded the proportion of false-positive results. However, many false-positive reactions make the use of the test a challenge.
Original languageEnglish
Article number2356
Number of pages13
Publication statusPublished - 9 Dec 2020

    Research areas

  • abomasal lesion, abomasal ulcer, bovine, dairy, diagnostic test, fecal occult blood

See relations at Aarhus University Citationformats

ID: 205897733