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Mathias Neumann Andersen

Cytosolic Glutamine Synthetase is Important for Photosynthetic Efficiency and Water Use Efficiency in Potato as Revealed by High Throughput Sequencing QTL analysis

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  • Kacper Piotr Kaminski, Department of Chemistry and Bioscience, Aalborg University, Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Denmark
  • Kirsten Kørup Sørensen
  • Mathias Neumann Andersen
  • Mads Sønderkær, Department of Biotechnology, Chemistry and Environmental Engineering, 9000 Aalborg University, Denmark
  • Mette Sondrup Andersen, Department of Chemistry and Bioscience, Aalborg University, Denmark
  • Hanne Grethe Kirk, Danish Potato Breeding Foundation, Denmark
  • Kåre Lehmann Nielsen, Department of Chemistry and Bioscience, Aalborg University, Denmark
Potato (Solanum tuberosum L.) closes its stomata at relatively low soil water deficits frequently encountered in normal field conditions resulting in unnecessary annual yield losses and extensive use of artificial irrigation. Therefore, unraveling the genetics underpinning variation in water use efficiency (WUE) of potato is important, but has been limited by technical difficulties in assessing the trait on individual plants and thus is poorly understood. In this study, a mapping population of potatoes has been robustly phenotyped, and considerable variation in WUE under well-watered conditions was observed. Two extreme WUE bulks of clones were identified and pools of genomic DNA from them as well as the parents were sequenced and mapped to reference potato genome. Following a novel data analysis approach, two highly resolved QTLs were found on chromosome 1 and 9. Interestingly, three genes encoding isoforms of cytosolic glutamine synthase were located in the QTL at chromosome 1 suggesting a major contribution of this enzyme to photosynthetic efficiency and thus WUE in potato. Indeed, Glutamine synthetase enzyme activity of leaf extracts was measured and found to be correlated with contrasting WUE phenotypes.
Original languageEnglish
JournalTheoretical and Applied Genetics
Volume128
Issue11
Pages (from-to)2143-2153
ISSN0040-5752
DOIs
Publication statusPublished - 2015

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