Department of Biology

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Mark Lever

Growth of sedimentary Bathyarchaeota on lignin as an energy source

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

DOI

  • Tiantian Yu, Shanghai Jiao Tong University
  • ,
  • Weichao Wu, University of Bremen
  • ,
  • Wenyue Liang, Shanghai Jiao Tong University
  • ,
  • Mark Alexander Lever
  • Kai Uwe Hinrichs, University of Bremen
  • ,
  • Fengping Wang, Shanghai Jiao Tong University

Members of the archaeal phylum Bathyarchaeota are among the most abundant microorganisms on Earth. Although versatile metabolic capabilities such as acetogenesis, methanogenesis, and fermentation have been suggested for bathyarchaeotal members, no direct confirmation of these metabolic functions has been achieved through growth of Bathyarchaeota in the laboratory. Here we demonstrate, on the basis of gene-copy numbers and probing of archaeal lipids, the growth of Bathyarchaeota subgroup Bathy-8 in enrichments of estuarine sediments with the biopolymer lignin. Other organic substrates (casein, oleic acid, cellulose, and phenol) did not significantly stimulate growth of Bathyarchaeota. Meanwhile, putative bathyarchaeotal tetraether lipids incorporated13C from13C-bicarbonate only when added in concert with lignin. Our results are consistent with organoautotrophic growth of a bathyarchaeotal group with lignin as an energy source and bicarbonate as a carbon source and shed light into the cycling of one of Earth’s most abundant biopolymers in anoxic marine sediment.

Original languageEnglish
JournalProceedings of the National Academy of Sciences of the United States of America
Volume115
Issue23
Pages (from-to)6022-6027
Number of pages6
ISSN0027-8424
DOIs
Publication statusPublished - 5 Jun 2018
Externally publishedYes

Bibliographical note

Funding Information:
We thank Karl O. Stetter (University of Regensburg) and Hongbo Yu (Huazhong University of Science and Technology) for kind suggestions to improve the manuscript and Jiahua Wang (Shanghai Jiao Tong University) for help with the metagenome analysis. This work was supported financially by State Key Research and Development Project of China Grant 2016YFA0601102, Natural Science Foundation of China Grants 41525011, 91751205, and 91428308, and the Deutsche Forschungsgemein-schaft through the Gottfried Wilhelm Leibniz Program Award Hi 616-14-1 (to K.-U.H.). This study is also a contribution to the Integrated Marine Biosphere Research International Project and the Deep Carbon Observatory.

Funding Information:
ACKNOWLEDGMENTS. We thank Karl O. Stetter (University of Regensburg) and Hongbo Yu (Huazhong University of Science and Technology) for kind suggestions to improve the manuscript and Jiahua Wang (Shanghai Jiao Tong University) for help with the metagenome analysis. This work was supported financially by State Key Research and Development Project of China Grant 2016YFA0601102, Natural Science Foundation of China Grants 41525011, 91751205, and 91428308, and the Deutsche Forschungsgemein-schaft through the Gottfried Wilhelm Leibniz Program Award Hi 616-14-1 (to K.-U.H.). This study is also a contribution to the Integrated Marine Biosphere Research International Project and the Deep Carbon Observatory.

Publisher Copyright:
© 2018 National Academy of Sciences. All Rights Reserved.

Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.

    Research areas

  • Bathyarchaeota, Carbon fixation, Lignin degradation, Lipid, Sedimentary carbon cycling

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