Marco Capogna

Organotypic slice cultures: a technique has come of age

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperReviewResearchpeer-review

Standard

Organotypic slice cultures : a technique has come of age. / Gähwiler, B H; Capogna, M; Debanne, D et al.

In: Trends in Neurosciences, Vol. 20, No. 10, 10.1997, p. 471-7.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperReviewResearchpeer-review

Harvard

Gähwiler, BH, Capogna, M, Debanne, D, McKinney, RA & Thompson, SM 1997, 'Organotypic slice cultures: a technique has come of age', Trends in Neurosciences, vol. 20, no. 10, pp. 471-7.

APA

Gähwiler, B. H., Capogna, M., Debanne, D., McKinney, R. A., & Thompson, S. M. (1997). Organotypic slice cultures: a technique has come of age. Trends in Neurosciences, 20(10), 471-7.

CBE

Gähwiler BH, Capogna M, Debanne D, McKinney RA, Thompson SM. 1997. Organotypic slice cultures: a technique has come of age. Trends in Neurosciences. 20(10):471-7.

MLA

Gähwiler, B H et al. "Organotypic slice cultures: a technique has come of age". Trends in Neurosciences. 1997, 20(10). 471-7.

Vancouver

Gähwiler BH, Capogna M, Debanne D, McKinney RA, Thompson SM. Organotypic slice cultures: a technique has come of age. Trends in Neurosciences. 1997 Oct;20(10):471-7.

Author

Gähwiler, B H ; Capogna, M ; Debanne, D et al. / Organotypic slice cultures : a technique has come of age. In: Trends in Neurosciences. 1997 ; Vol. 20, No. 10. pp. 471-7.

Bibtex

@article{021a560ef35f42f7818dbb611c5f6e4e,
title = "Organotypic slice cultures: a technique has come of age",
abstract = "Slices of CNS tissue prepared from young rodents can be maintained in culture for many weeks to months. The basic requirements are simple: a stable substratum, culture medium, sufficient oxygenation and incubation at a temperature of about 36 degrees C. Under these conditions, nerve cells continue to differentiate and to develop a tissue organization that closely resembles that observed in situ. Several alternative culturing methods have been developed recently. Slices maintained in stationary culture with the interface method are ideally suited for questions requiring a three-dimensional structure, whereas slices cultured in roller-tubes remain the method of choice for experiments that require optimal optical conditions. In this report, three typical experiments are discussed that illustrate the potential of the slice-culture technique. The first example indicates that, due to their high neuronal connectivity, slice cultures provide a very useful tool for studying the properties of synaptic transmission between monosynaptically coupled cell pairs. The other two studies show how long-term application of substances to slice cultures can be used to examine the consequences of epileptic discharges in vitro, as well as the effects of slowly acting clostridial neurotoxins on synaptic transmission.",
keywords = "Animals, Humans, Neurology/methods, Organ Culture Techniques",
author = "G{\"a}hwiler, {B H} and M Capogna and D Debanne and McKinney, {R A} and Thompson, {S M}",
year = "1997",
month = oct,
language = "English",
volume = "20",
pages = "471--7",
journal = "Trends in Neurosciences",
issn = "0166-2236",
publisher = "Elsevier Ltd. * Trends Journals",
number = "10",

}

RIS

TY - JOUR

T1 - Organotypic slice cultures

T2 - a technique has come of age

AU - Gähwiler, B H

AU - Capogna, M

AU - Debanne, D

AU - McKinney, R A

AU - Thompson, S M

PY - 1997/10

Y1 - 1997/10

N2 - Slices of CNS tissue prepared from young rodents can be maintained in culture for many weeks to months. The basic requirements are simple: a stable substratum, culture medium, sufficient oxygenation and incubation at a temperature of about 36 degrees C. Under these conditions, nerve cells continue to differentiate and to develop a tissue organization that closely resembles that observed in situ. Several alternative culturing methods have been developed recently. Slices maintained in stationary culture with the interface method are ideally suited for questions requiring a three-dimensional structure, whereas slices cultured in roller-tubes remain the method of choice for experiments that require optimal optical conditions. In this report, three typical experiments are discussed that illustrate the potential of the slice-culture technique. The first example indicates that, due to their high neuronal connectivity, slice cultures provide a very useful tool for studying the properties of synaptic transmission between monosynaptically coupled cell pairs. The other two studies show how long-term application of substances to slice cultures can be used to examine the consequences of epileptic discharges in vitro, as well as the effects of slowly acting clostridial neurotoxins on synaptic transmission.

AB - Slices of CNS tissue prepared from young rodents can be maintained in culture for many weeks to months. The basic requirements are simple: a stable substratum, culture medium, sufficient oxygenation and incubation at a temperature of about 36 degrees C. Under these conditions, nerve cells continue to differentiate and to develop a tissue organization that closely resembles that observed in situ. Several alternative culturing methods have been developed recently. Slices maintained in stationary culture with the interface method are ideally suited for questions requiring a three-dimensional structure, whereas slices cultured in roller-tubes remain the method of choice for experiments that require optimal optical conditions. In this report, three typical experiments are discussed that illustrate the potential of the slice-culture technique. The first example indicates that, due to their high neuronal connectivity, slice cultures provide a very useful tool for studying the properties of synaptic transmission between monosynaptically coupled cell pairs. The other two studies show how long-term application of substances to slice cultures can be used to examine the consequences of epileptic discharges in vitro, as well as the effects of slowly acting clostridial neurotoxins on synaptic transmission.

KW - Animals

KW - Humans

KW - Neurology/methods

KW - Organ Culture Techniques

M3 - Review

C2 - 9347615

VL - 20

SP - 471

EP - 477

JO - Trends in Neurosciences

JF - Trends in Neurosciences

SN - 0166-2236

IS - 10

ER -