Marco Capogna

Neurogliaform cells of amygdala: a source of slow phasic inhibition in the basolateral complex

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Mirosława Mańko, MRC Anatomical Neuropharmacology Unit, Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3TH, UK. Electronic address: marco.capogna@pharm.ox.ac.uk.
  • ,
  • Thomas C M Bienvenu
  • ,
  • Yannis Dalezios
  • ,
  • Marco Capogna

Synaptic inhibition in the amygdala actively participates in processing emotional information. To improve the understanding of interneurons in amygdala networks it is necessary to characterize the GABAergic cell types, their connectivity and physiological roles. We used a mouse line expressing a green fluorescent protein (GFP) under the neuropeptide Y (NPY) promoter. Paired recordings between presynaptic NPY-GFP-expressing (+) cells and postsynaptic principal neurons (PNs) of the basolateral amygdala (BLA) were performed. The NPY-GFP+ neurons displayed small somata and short dendrites embedded in a cloud of highly arborized axon, suggesting a neurogliaform cell (NGFC) type. We discovered that a NPY-GFP+ cell evoked a GABA(A) receptor-mediated slow inhibitory postsynaptic current (IPSC) in a PN and an autaptic IPSC. The slow kinetics of these IPSCs was likely caused by the low concentration and spillover of extracellular GABA. We also report that NGFCs of the BLA fired action potentials phase-locked to hippocampal theta oscillations in anaesthetized rats. When this firing was re-played in NPY+-NGFCs in vitro, it evoked a transient depression of the IPSCs. Presynaptic GABA(B) receptors and functional depletion of synaptic vesicles determined this short-term plasticity. Synaptic contacts made by recorded NGFCs showed close appositions, and rarely identifiable classical synaptic structures. Thus, we report here a novel interneuron type of the amygdala that generates volume transmission of GABA. The peculiar functional mode of NGFCs makes them unique amongst all GABAergic cell types of the amygdala identified so far.

Original languageEnglish
JournalThe Journal of Physiology
Volume590
Issue22
Pages (from-to)5611-27
Number of pages17
ISSN0022-3751
DOIs
Publication statusPublished - 15 Nov 2012

    Research areas

  • Action Potentials/drug effects, Amygdala/cytology, Animals, Axons/physiology, Dendrites/physiology, GABA Antagonists/pharmacology, GABAergic Neurons/classification, Inhibitory Postsynaptic Potentials, Interneurons/classification, Neuronal Plasticity, Rats, Synapses/physiology, Synaptic Transmission, Theta Rhythm, gamma-Aminobutyric Acid/metabolism

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