Marco Capogna

Different fear states engage distinct networks within the intercalated cell clusters of the amygdala

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Different fear states engage distinct networks within the intercalated cell clusters of the amygdala. / Busti, Daniela; Geracitano, Raffaella; Whittle, Nigel; Dalezios, Yannis; Mańko, Miroslawa; Kaufmann, Walter; Sätzler, Kurt; Singewald, Nicolas; Capogna, Marco; Ferraguti, Francesco.

In: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, No. 13, 30.03.2011, p. 5131-44.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Busti, D, Geracitano, R, Whittle, N, Dalezios, Y, Mańko, M, Kaufmann, W, Sätzler, K, Singewald, N, Capogna, M & Ferraguti, F 2011, 'Different fear states engage distinct networks within the intercalated cell clusters of the amygdala', The Journal of neuroscience : the official journal of the Society for Neuroscience, vol. 31, no. 13, pp. 5131-44. https://doi.org/10.1523/JNEUROSCI.6100-10.2011

APA

Busti, D., Geracitano, R., Whittle, N., Dalezios, Y., Mańko, M., Kaufmann, W., Sätzler, K., Singewald, N., Capogna, M., & Ferraguti, F. (2011). Different fear states engage distinct networks within the intercalated cell clusters of the amygdala. The Journal of neuroscience : the official journal of the Society for Neuroscience, 31(13), 5131-44. https://doi.org/10.1523/JNEUROSCI.6100-10.2011

CBE

Busti D, Geracitano R, Whittle N, Dalezios Y, Mańko M, Kaufmann W, Sätzler K, Singewald N, Capogna M, Ferraguti F. 2011. Different fear states engage distinct networks within the intercalated cell clusters of the amygdala. The Journal of neuroscience : the official journal of the Society for Neuroscience. 31(13):5131-44. https://doi.org/10.1523/JNEUROSCI.6100-10.2011

MLA

Busti, Daniela et al. "Different fear states engage distinct networks within the intercalated cell clusters of the amygdala". The Journal of neuroscience : the official journal of the Society for Neuroscience. 2011, 31(13). 5131-44. https://doi.org/10.1523/JNEUROSCI.6100-10.2011

Vancouver

Busti D, Geracitano R, Whittle N, Dalezios Y, Mańko M, Kaufmann W et al. Different fear states engage distinct networks within the intercalated cell clusters of the amygdala. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2011 Mar 30;31(13):5131-44. https://doi.org/10.1523/JNEUROSCI.6100-10.2011

Author

Busti, Daniela ; Geracitano, Raffaella ; Whittle, Nigel ; Dalezios, Yannis ; Mańko, Miroslawa ; Kaufmann, Walter ; Sätzler, Kurt ; Singewald, Nicolas ; Capogna, Marco ; Ferraguti, Francesco. / Different fear states engage distinct networks within the intercalated cell clusters of the amygdala. In: The Journal of neuroscience : the official journal of the Society for Neuroscience. 2011 ; Vol. 31, No. 13. pp. 5131-44.

Bibtex

@article{aac3ba5360b042a9b24b33d8ed824b56,
title = "Different fear states engage distinct networks within the intercalated cell clusters of the amygdala",
abstract = "Although extinction-based therapies are among the most effective treatments for anxiety disorders, the neural bases of fear extinction remain still essentially unclear. Recent evidence suggests that the intercalated cell masses of the amygdala (ITCs) are critical structures for fear extinction. However, the neuronal organization of ITCs and how distinct clusters contribute to different fear states are still entirely unknown. Here, by combining whole-cell patch-clamp recordings and biocytin labeling with full anatomical reconstruction of the filled neurons and ultrastructural analysis of their synaptic contacts, we have elucidated the cellular organization and efferent connections of one of the main ITC clusters in mice. Our data showed an unexpected heterogeneity in the axonal pattern of medial paracapsular ITC (Imp) neurons and the presence of three distinct neuronal subtypes. Functionally, we observed that the Imp was preferentially activated during fear expression, whereas extinction training and extinction retrieval activated the main ITC nucleus (IN), as measured by quantifying Zif268 expression. This can be explained by the IPSPs evoked in the IN after Imp stimulation, most likely through the GABAergic monosynaptic innervation of IN neurons by one subtype of Imp cells, namely the medial capsular-projecting (MCp)-Imp neurons. MCp-Imp neurons also target large ITC cells that surround ITC clusters and express the metabotropic glutamate receptor 1α. These findings reveal a distinctive participation of ITC clusters to different fear states and the underlying anatomical circuitries, hence shedding new light on ITC networks and providing a novel framework to elucidate their role in fear expression and extinction.",
keywords = "Amygdala/cytology, Animals, Axons/physiology, Cell Communication/physiology, Fear/physiology, Interneurons/cytology, Male, Mice, Mice, Inbred C57BL, Mice, Transgenic, Nerve Net/cytology",
author = "Daniela Busti and Raffaella Geracitano and Nigel Whittle and Yannis Dalezios and Miroslawa Ma{\'n}ko and Walter Kaufmann and Kurt S{\"a}tzler and Nicolas Singewald and Marco Capogna and Francesco Ferraguti",
year = "2011",
month = mar,
day = "30",
doi = "10.1523/JNEUROSCI.6100-10.2011",
language = "English",
volume = "31",
pages = "5131--44",
journal = "The Journal of neuroscience : the official journal of the Society for Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "13",

}

RIS

TY - JOUR

T1 - Different fear states engage distinct networks within the intercalated cell clusters of the amygdala

AU - Busti, Daniela

AU - Geracitano, Raffaella

AU - Whittle, Nigel

AU - Dalezios, Yannis

AU - Mańko, Miroslawa

AU - Kaufmann, Walter

AU - Sätzler, Kurt

AU - Singewald, Nicolas

AU - Capogna, Marco

AU - Ferraguti, Francesco

PY - 2011/3/30

Y1 - 2011/3/30

N2 - Although extinction-based therapies are among the most effective treatments for anxiety disorders, the neural bases of fear extinction remain still essentially unclear. Recent evidence suggests that the intercalated cell masses of the amygdala (ITCs) are critical structures for fear extinction. However, the neuronal organization of ITCs and how distinct clusters contribute to different fear states are still entirely unknown. Here, by combining whole-cell patch-clamp recordings and biocytin labeling with full anatomical reconstruction of the filled neurons and ultrastructural analysis of their synaptic contacts, we have elucidated the cellular organization and efferent connections of one of the main ITC clusters in mice. Our data showed an unexpected heterogeneity in the axonal pattern of medial paracapsular ITC (Imp) neurons and the presence of three distinct neuronal subtypes. Functionally, we observed that the Imp was preferentially activated during fear expression, whereas extinction training and extinction retrieval activated the main ITC nucleus (IN), as measured by quantifying Zif268 expression. This can be explained by the IPSPs evoked in the IN after Imp stimulation, most likely through the GABAergic monosynaptic innervation of IN neurons by one subtype of Imp cells, namely the medial capsular-projecting (MCp)-Imp neurons. MCp-Imp neurons also target large ITC cells that surround ITC clusters and express the metabotropic glutamate receptor 1α. These findings reveal a distinctive participation of ITC clusters to different fear states and the underlying anatomical circuitries, hence shedding new light on ITC networks and providing a novel framework to elucidate their role in fear expression and extinction.

AB - Although extinction-based therapies are among the most effective treatments for anxiety disorders, the neural bases of fear extinction remain still essentially unclear. Recent evidence suggests that the intercalated cell masses of the amygdala (ITCs) are critical structures for fear extinction. However, the neuronal organization of ITCs and how distinct clusters contribute to different fear states are still entirely unknown. Here, by combining whole-cell patch-clamp recordings and biocytin labeling with full anatomical reconstruction of the filled neurons and ultrastructural analysis of their synaptic contacts, we have elucidated the cellular organization and efferent connections of one of the main ITC clusters in mice. Our data showed an unexpected heterogeneity in the axonal pattern of medial paracapsular ITC (Imp) neurons and the presence of three distinct neuronal subtypes. Functionally, we observed that the Imp was preferentially activated during fear expression, whereas extinction training and extinction retrieval activated the main ITC nucleus (IN), as measured by quantifying Zif268 expression. This can be explained by the IPSPs evoked in the IN after Imp stimulation, most likely through the GABAergic monosynaptic innervation of IN neurons by one subtype of Imp cells, namely the medial capsular-projecting (MCp)-Imp neurons. MCp-Imp neurons also target large ITC cells that surround ITC clusters and express the metabotropic glutamate receptor 1α. These findings reveal a distinctive participation of ITC clusters to different fear states and the underlying anatomical circuitries, hence shedding new light on ITC networks and providing a novel framework to elucidate their role in fear expression and extinction.

KW - Amygdala/cytology

KW - Animals

KW - Axons/physiology

KW - Cell Communication/physiology

KW - Fear/physiology

KW - Interneurons/cytology

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Transgenic

KW - Nerve Net/cytology

U2 - 10.1523/JNEUROSCI.6100-10.2011

DO - 10.1523/JNEUROSCI.6100-10.2011

M3 - Journal article

C2 - 21451049

VL - 31

SP - 5131

EP - 5144

JO - The Journal of neuroscience : the official journal of the Society for Neuroscience

JF - The Journal of neuroscience : the official journal of the Society for Neuroscience

SN - 0270-6474

IS - 13

ER -