Louise Feld

Selective pressure affects transfer and establishment of a Lactobacillus plantarum resistance plasmid in the gastrointestinal environment

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

DOI

  • Louise Feld
  • Susanne Schjørring, Statens Serum Institut
  • ,
  • Karin Hammer, Technical University of Denmark
  • ,
  • Tine Rask Licht, Technical University of Denmark
  • ,
  • Morten Danielsen, Chr. Hansen A/S
  • ,
  • Karen Krogfelt, Statens Serum Institut
  • ,
  • Andrea Wilcks, Technical University of Denmark

Objectives and methods: A Lactobacillus plantarum strain recently isolated from French raw-milk cheese was tested for its ability to transfer a small plasmid pLFE1 harbouring the erythromycin resistance gene erm (B) to Enterococcus faecalis. Mating was studied in vitro and in different gastrointestinal environments using gnotobiotic rats as a simple in vivo model and streptomycin-treated mice as a more complex model. Transfer and establishment of transconjugants in the intestine were investigated with and without selective pressure. Results: Compared with the relatively low transfer frequency of ∼5.7 × 10-8 transconjugants/recipient obtained in vitro by filter mating, a surprisingly high number of transconjugants (10-4 transconjugants/recipient) was observed in gnotobiotic rats even without antibiotic treatment. When erythromycin was administered, a transfer rate of ∼100% was observed, i.e. the recipient population turned completely into transconjugants (3 × 109 cfu/g faeces). Additionally, the time to reach a stable transconjugant population level was much faster in the erythromycin-treated gnotobiotic rats (1 day) than in the untreated animals (4-5 days). Transconjugants persisted in the gut in relatively stable numbers at least 12 days after termination of antibiotic treatment. In the streptomycin-treated mice, no transfer was observed either with or without erythromycin treatment. Conclusions: The overall results imply that the gastrointestinal tract may comprise a more favourable environment for antibiotic resistance transfer than conditions provided in vitro. However, the indigenous gut microbiota severely restricts transfer, thus minimizing the number of detectable transfer events. Treatment with erythromycin strongly favoured transfer and establishment of pLFE1.

Original languageEnglish
JournalJournal of Antimicrobial Chemotherapy
Volume61
Issue4
Pages (from-to)845-852
Number of pages8
ISSN0305-7453
DOIs
Publication statusPublished - Apr 2008
Externally publishedYes

    Research areas

  • Antibiotic resistance, Gastrointestinal tract, Horizontal gene transfer, L. plantarum

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