Louise Feld

Horizontal transfer of tet(M) and erm(B) resistance plasmids from food strains of Lactobacillus plantarum to Enterococcus faecalis JH2-2 in the gastrointestinal tract of gnotobiotic rats

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Louise Jacobsen
  • Andrea Wilcks, Technical University of Denmark
  • ,
  • Karin Hammer, Technical University of Denmark
  • ,
  • Geert Huys, Ghent University
  • ,
  • Dirk Gevers, Ghent University, Hogeschool Gent and Universiteit Gent
  • ,
  • Sigrid Rita Andersen, Technical University of Denmark, University College Øresund

Two wild-type strains of Lactobacillus plantarum previously isolated from fermented dry sausages were analysed for their ability to transfer antibiotic resistance plasmids in the gastrointestinal tract. For this purpose, we used gnotobiotic rats as an in vivo model. Rats were initially inoculated with the recipient Enterococcus faecalis JH2-2 at a concentration of 1010 CFU mL-1. After a week, either of the two donors L. plantarum DG 522 (harbouring a tet(M)-containing plasmid of c. 40 kb) or L. plantarum DG 507 [harbouring a tet(M)-containing plasmid of c. 10 kb and an erm(B)-containing plasmid of c. 8.5 kb] was introduced at concentrations in the range of 10 8-1010 CFU mL-1. Two days after donor introduction, the first transconjugants (TCs) were detected in faecal samples. The detected numbers of tet(M)-TCs were comparable for the two donors. In both cases, this number increased to c. 5 × 102 CFU g-1 faeces towards the end of the experiment. For erm(B)-TCs, the number was significantly higher and increased to c. 103 CFU g-1 faeces. To our knowledge, this is the first study showing in vivo transfer of wild-type antibiotic resistance plasmids from L. plantarum to E. faecalis.

Original languageEnglish
JournalFEMS Microbiology Ecology
Volume59
Issue1
Pages (from-to)158-166
Number of pages9
ISSN0168-6496
DOIs
Publication statusPublished - Jan 2007
Externally publishedYes

    Research areas

  • Antibiotic resistance, Gastrointestinal tract, Gnotobiotic rats, Horizontal gene transfer, Lactobacillus plantarum

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