Lise Lotte Hansen

Sensitive and fast mutation detection by solid phase chemical cleavage

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

We have developed a solid phase chemical cleavage method (SpCCM) for screening large DNA fragments for mutations. All reactions can be carried out in microtiterwells from the first amplification of the patient (or test) DNA through the search for mutations. The reaction time is significantly reduced compared to the conventional chemical cleavage method (CCM), and even by using a uniformly labelled probe, the exact position and nature of the mutation can be revealed. The SpCCM is suitable for automatization using a workstation to carry out the reactions and a fluorescent detection-based DNA sequencing system to analyze the cleaved fragments
Original languageEnglish
JournalHuman Mutation
Volume7
Issue3
Pages (from-to)256-263
Number of pages8
ISSN1059-7794
DOIs
Publication statusPublished - 1996

    Research areas

  • mutation detection, chemical cleavage

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