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Lene Pedersen

A correlation between dexamethasone inducibility and basal expression levels of retroviral vector proviruses

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Standard

A correlation between dexamethasone inducibility and basal expression levels of retroviral vector proviruses. / Duch, M; Paludan, K; Lovmand, J; Pedersen, L; Jørgensen, Poul; Pedersen, F S.

In: Nucleic Acids Research, Vol. 21, No. 20, 1993, p. 4777-82.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Duch, M, Paludan, K, Lovmand, J, Pedersen, L, Jørgensen, P & Pedersen, FS 1993, 'A correlation between dexamethasone inducibility and basal expression levels of retroviral vector proviruses', Nucleic Acids Research, vol. 21, no. 20, pp. 4777-82.

APA

Duch, M., Paludan, K., Lovmand, J., Pedersen, L., Jørgensen, P., & Pedersen, F. S. (1993). A correlation between dexamethasone inducibility and basal expression levels of retroviral vector proviruses. Nucleic Acids Research, 21(20), 4777-82.

CBE

Duch M, Paludan K, Lovmand J, Pedersen L, Jørgensen P, Pedersen FS. 1993. A correlation between dexamethasone inducibility and basal expression levels of retroviral vector proviruses. Nucleic Acids Research. 21(20):4777-82.

MLA

Vancouver

Duch M, Paludan K, Lovmand J, Pedersen L, Jørgensen P, Pedersen FS. A correlation between dexamethasone inducibility and basal expression levels of retroviral vector proviruses. Nucleic Acids Research. 1993;21(20):4777-82.

Author

Duch, M ; Paludan, K ; Lovmand, J ; Pedersen, L ; Jørgensen, Poul ; Pedersen, F S. / A correlation between dexamethasone inducibility and basal expression levels of retroviral vector proviruses. In: Nucleic Acids Research. 1993 ; Vol. 21, No. 20. pp. 4777-82.

Bibtex

@article{b6c5b270ab1711dd889c000ea68e967b,
title = "A correlation between dexamethasone inducibility and basal expression levels of retroviral vector proviruses",
abstract = "Identical transcription units inserted at different positions of mammalian chromosomes may vary widely in transcriptional activity. We have used a set of ten cell clones with random unselected single integrations of retroviral vectors to study such position effects. The vector used carries a neo gene driven by the Akv murine leukemia virus long terminal repeat that has only a weak promoter-enhancer activity in the target cell, the lymphoid cell line L691. Under transient expression conditions, the strength of the Akv promoter-enhancer in the L691 cells is increased by dexamethasone. In cell clones with single vector integrations, a correlation is observed between the non-induced expression levels and the degree of dexamethasone induction. The strongest relative induction is found for the integrated vectors with the lowest non-induced expression levels and approaches the inducibility under transient expression. These results indicate that expression levels are composed of distinct contributions from the integrated vector and from the site of integration and are best explained in terms of a model in which the sites of chromosomal integration exert variable positive enhancer effects upon vector transcription.",
keywords = "Animals, Base Sequence, Cell Line, DNA, Viral, Dexamethasone, Gene Expression Regulation, Viral, Genetic Vectors, Mice, Molecular Sequence Data, Promoter Regions (Genetics), Proviruses, Retroviridae, Transcription, Genetic",
author = "M Duch and K Paludan and J Lovmand and L Pedersen and Poul J{\o}rgensen and Pedersen, {F S}",
year = "1993",
language = "English",
volume = "21",
pages = "4777--82",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "20",

}

RIS

TY - JOUR

T1 - A correlation between dexamethasone inducibility and basal expression levels of retroviral vector proviruses

AU - Duch, M

AU - Paludan, K

AU - Lovmand, J

AU - Pedersen, L

AU - Jørgensen, Poul

AU - Pedersen, F S

PY - 1993

Y1 - 1993

N2 - Identical transcription units inserted at different positions of mammalian chromosomes may vary widely in transcriptional activity. We have used a set of ten cell clones with random unselected single integrations of retroviral vectors to study such position effects. The vector used carries a neo gene driven by the Akv murine leukemia virus long terminal repeat that has only a weak promoter-enhancer activity in the target cell, the lymphoid cell line L691. Under transient expression conditions, the strength of the Akv promoter-enhancer in the L691 cells is increased by dexamethasone. In cell clones with single vector integrations, a correlation is observed between the non-induced expression levels and the degree of dexamethasone induction. The strongest relative induction is found for the integrated vectors with the lowest non-induced expression levels and approaches the inducibility under transient expression. These results indicate that expression levels are composed of distinct contributions from the integrated vector and from the site of integration and are best explained in terms of a model in which the sites of chromosomal integration exert variable positive enhancer effects upon vector transcription.

AB - Identical transcription units inserted at different positions of mammalian chromosomes may vary widely in transcriptional activity. We have used a set of ten cell clones with random unselected single integrations of retroviral vectors to study such position effects. The vector used carries a neo gene driven by the Akv murine leukemia virus long terminal repeat that has only a weak promoter-enhancer activity in the target cell, the lymphoid cell line L691. Under transient expression conditions, the strength of the Akv promoter-enhancer in the L691 cells is increased by dexamethasone. In cell clones with single vector integrations, a correlation is observed between the non-induced expression levels and the degree of dexamethasone induction. The strongest relative induction is found for the integrated vectors with the lowest non-induced expression levels and approaches the inducibility under transient expression. These results indicate that expression levels are composed of distinct contributions from the integrated vector and from the site of integration and are best explained in terms of a model in which the sites of chromosomal integration exert variable positive enhancer effects upon vector transcription.

KW - Animals

KW - Base Sequence

KW - Cell Line

KW - DNA, Viral

KW - Dexamethasone

KW - Gene Expression Regulation, Viral

KW - Genetic Vectors

KW - Mice

KW - Molecular Sequence Data

KW - Promoter Regions (Genetics)

KW - Proviruses

KW - Retroviridae

KW - Transcription, Genetic

M3 - Journal article

C2 - 8233826

VL - 21

SP - 4777

EP - 4782

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 20

ER -