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Lene Pedersen

A correlation between dexamethasone inducibility and basal expression levels of retroviral vector proviruses

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  • M Duch, Denmark
  • K Paludan, Denmark
  • J Lovmand, Denmark
  • L Pedersen
  • Poul Jørgensen, Denmark
  • F S Pedersen, Denmark
  • Interdisciplinary Nanoscience Center
  • Department of Science Studies
  • Department of Molecular Biology
Identical transcription units inserted at different positions of mammalian chromosomes may vary widely in transcriptional activity. We have used a set of ten cell clones with random unselected single integrations of retroviral vectors to study such position effects. The vector used carries a neo gene driven by the Akv murine leukemia virus long terminal repeat that has only a weak promoter-enhancer activity in the target cell, the lymphoid cell line L691. Under transient expression conditions, the strength of the Akv promoter-enhancer in the L691 cells is increased by dexamethasone. In cell clones with single vector integrations, a correlation is observed between the non-induced expression levels and the degree of dexamethasone induction. The strongest relative induction is found for the integrated vectors with the lowest non-induced expression levels and approaches the inducibility under transient expression. These results indicate that expression levels are composed of distinct contributions from the integrated vector and from the site of integration and are best explained in terms of a model in which the sites of chromosomal integration exert variable positive enhancer effects upon vector transcription.
Original languageEnglish
JournalNucleic Acids Research
Pages (from-to)4777-82
Number of pages5
Publication statusPublished - 1993

    Research areas

  • Animals, Base Sequence, Cell Line, DNA, Viral, Dexamethasone, Gene Expression Regulation, Viral, Genetic Vectors, Mice, Molecular Sequence Data, Promoter Regions (Genetics), Proviruses, Retroviridae, Transcription, Genetic

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