Leif Østergaard

Preserved cerebral microcirculation after cardiac arrest in a rat model

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

OBJECTIVE: Recent studies show that sublingual microcirculation is altered in patients resuscitated from cardiac arrest (CA). The objective of this study was to investigate whether the cerebral microcirculation is disturbed in the early post resuscitation period.

METHODS: Male Sprague-Dawley rats were randomized to either 10 min of CA or uninterrupted circulation, and observed for 120 or 360min after return of spontaneous circulation (ROSC). At 120 and 360min, cerebral microcirculation was evaluated by side stream dark field microscopy (SDF) through a craniotomy. Plasma samples were analyzed for endothelial adhesion molecules and inflammatory markers, and brains were fixated for histological analysis.

RESULTS: Cerebral microcirculation, evaluated by total vessel density, perfused vessel density, proportion of perfused vessels, and microvascular flow index did not differ between groups (p>0.16). Plasma samples drawn 360 min after ROSC displayed a significant increase in sE-selectin, sL-selectin, sI-CAM1, IL-1β, IL-6, IL-10, and elastase compared to controls. In the CA animals, sE-selectin and elastase increased between 120 and 360 min after resuscitation (p<0.007). Histological analysis revealed neuronal death in hippocampus layer CA1.

CONCLUSION: When evaluated by SDF, the cerebral microcirculation appears unaffected in the early post CA period despite hypotension, systemic inflammation, endothelial activation, and neuronal injury. This article is protected by copyright. All rights reserved.

Original languageEnglish
JournalMicrocirculation (New York, N.Y. : 1994)
Pages (from-to)464-74
Number of pages11
Publication statusPublished - 1 Aug 2015

See relations at Aarhus University Citationformats

ID: 90161607