Leif Østergaard

More homogeneous capillary flow and oxygenation in deeper cortical layers correlate with increased oxygen extraction

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

DOI

  • Baoqiang Lu, Harvard Med Sch, Harvard University, VA Boston Healthcare System, Massachusetts General Hospital, Massachusetts Gen Hosp, Athinoula A Martinos Ctr Biomed Imaging
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  • Tatiana Esipova, Univ Penn, University of Pennsylvania, Dept Chem
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  • Ikbal Sencan, Harvard Med Sch, Harvard University, VA Boston Healthcare System, Massachusetts General Hospital, Massachusetts Gen Hosp, Athinoula A Martinos Ctr Biomed Imaging
  • ,
  • Kivilcim Kilic, Univ Calif San Diego, University of California System, University of California San Diego, Dept Neurosci
  • ,
  • Buyin Fu, Harvard Med Sch, Harvard University, VA Boston Healthcare System, Massachusetts General Hospital, Massachusetts Gen Hosp, Athinoula A Martinos Ctr Biomed Imaging
  • ,
  • Michele Desjardins, Univ Calif San Diego, University of California System, University of California San Diego, Dept Radiol
  • ,
  • Mohammad Moeini, Montreal Heart Inst, University of Montreal, Res Ctr
  • ,
  • Sreekanth Kura, Harvard Med Sch, Harvard University, VA Boston Healthcare System, Massachusetts General Hospital, Massachusetts Gen Hosp, Athinoula A Martinos Ctr Biomed Imaging
  • ,
  • Mohammad A. Yaseen, Harvard Med Sch, Harvard University, VA Boston Healthcare System, Massachusetts General Hospital, Massachusetts Gen Hosp, Athinoula A Martinos Ctr Biomed Imaging
  • ,
  • Frederic Lesage, Montreal Heart Inst, University of Montreal, Res Ctr
  • ,
  • Leif Ostergaard
  • Anna Devor, Univ Calif San Diego, University of California System, University of California San Diego, Dept Radiol
  • ,
  • David A. Boas
  • Sergei A. Vinogradov, Univ Penn, University of Pennsylvania, Dept Chem
  • ,
  • Sava Sakadzic, Harvard Med Sch, Harvard University, VA Boston Healthcare System, Massachusetts General Hospital, Massachusetts Gen Hosp, Athinoula A Martinos Ctr Biomed Imaging

Our understanding of how capillary blood flow and oxygen distribute across cortical layers to meet the local metabolic demand is incomplete. We addressed this question by using two-photon imaging of resting-state microvascular oxygen partial pressure (PO2) and flow in the whisker barrel cortex in awake mice. Our measurements in layers I-V show that the capillary red-blood-cell flux and oxygenation heterogeneity, and the intracapillary resistance to oxygen delivery, all decrease with depth, reaching a minimum around layer IV, while the depth-dependent oxygen extraction fraction is increased in layer IV, where oxygen demand is presumably the highest. Our findings suggest that more homogeneous distribution of the physiological observables relevant to oxygen transport to tissue is an important part of the microvascular network adaptation to local brain metabolism. These results will inform the biophysical models of layer-specific cerebral oxygen delivery and consumption and improve our understanding of the diseases that affect cerebral microcirculation.

Original languageEnglish
Article number42299
JournaleLife
Volume8
Number of pages28
ISSN2050-084X
DOIs
Publication statusPublished - 2019

    Research areas

  • TRANSIT-TIME HETEROGENEITY, CEREBRAL-BLOOD-FLOW, ERYTHROCYTE-ASSOCIATED TRANSIENTS, LOCAL NEURONAL-ACTIVITY, SMALL VESSEL DISEASE, ALZHEIMERS-DISEASE, OPTOGENETIC STIMULATION, STRUCTURAL ADAPTATION, BRAIN OXYGENATION, VASCULAR NETWORK

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