Lars-Erik Holm

Whole-Genome de novo Sequencing Of Quail And Grey Partridge

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  • Lars-Erik Holm
  • Frank Panitz
  • Dave Burt, Division of Genetics and Genomics, The Roslin Institute, University of Edinburgh, United Kingdom
  • Richard Talbot, ARK-Genomics, The Roslin Institute, University of Edinburgh, United Kingdom
  • Sarah Smith, ARK-Genomics, The Roslin Institute, University of Edinburgh, United Kingdom
  • Liselotte Wesley Andersen
  • Christian Bendixen, Denmark
The development in sequencing methods has made it possible to perform whole genome de novo sequencing of species without large commercial interests. Within the EU-financed QUANTOMICS project (KBBE-2A-222664), we have performed de novo sequencing of quail (Coturnix coturnix) and grey partridge (Perdix perdix) on a Genome Analyzer GAII (Illumina) using paired-end sequencing. The amount of generated sequences amounts to 8 to 9 Gb for each species. The analysis and assembly of the generated sequences is ongoing. Access to the whole genome sequence from these two species will enable enhanced comparative studies towards the chicken genome and will aid in identifying evolutionarily conserved sequences within the Galliformes. The obtained sequences from quail and partridge represent a beginning of generating the whole genome sequence for these species. The continuation of establishing the genome sequence of these species will require generation of more sequences from more diverse libraries. The QUANTOMICS group encourages other research groups to participate in a joint effort to establish the genome sequence of these two Galliformes species.

Original languageEnglish
Publication year2011
Number of pages1
Publication statusPublished - 2011

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