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Lars Bolund

EWS and FUS bind a subset of transcribed genes encoding proteins enriched in RNA regulatory functions

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Standard

EWS and FUS bind a subset of transcribed genes encoding proteins enriched in RNA regulatory functions. / Luo, Yonglun; Friis, Jenny Blechingberg; Fernandes, Ana Miguel et al.
In: BMC Genomics, Vol. 16, 11.2015.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Harvard

Luo, Y, Friis, JB, Fernandes, AM, Li, S, Fryland, T, Børglum, A, Bolund, L & Nielsen, AL 2015, 'EWS and FUS bind a subset of transcribed genes encoding proteins enriched in RNA regulatory functions', BMC Genomics, vol. 16.

APA

Luo, Y., Friis, J. B., Fernandes, A. M., Li, S., Fryland, T., Børglum, A., Bolund, L., & Nielsen, A. L. (2015). EWS and FUS bind a subset of transcribed genes encoding proteins enriched in RNA regulatory functions. BMC Genomics, 16.

CBE

Luo Y, Friis JB, Fernandes AM, Li S, Fryland T, Børglum A, Bolund L, Nielsen AL. 2015. EWS and FUS bind a subset of transcribed genes encoding proteins enriched in RNA regulatory functions. BMC Genomics. 16.

MLA

Luo, Yonglun et al. "EWS and FUS bind a subset of transcribed genes encoding proteins enriched in RNA regulatory functions". BMC Genomics. 2015. 16.

Vancouver

Luo Y, Friis JB, Fernandes AM, Li S, Fryland T, Børglum A et al. EWS and FUS bind a subset of transcribed genes encoding proteins enriched in RNA regulatory functions. BMC Genomics. 2015 Nov;16.

Author

Luo, Yonglun ; Friis, Jenny Blechingberg ; Fernandes, Ana Miguel et al. / EWS and FUS bind a subset of transcribed genes encoding proteins enriched in RNA regulatory functions. In: BMC Genomics. 2015 ; Vol. 16.

Bibtex

@article{5df3612b871742cfb79d7c00bdc68a05,
title = "EWS and FUS bind a subset of transcribed genes encoding proteins enriched in RNA regulatory functions",
abstract = "BackgroundFUS (TLS) and EWS (EWSR1) belong to the FET-protein family of RNA and DNA binding proteins. FUS and EWS are structurally and functionally related and participate in transcriptional regulation and RNA processing. FUS and EWS are identified in translocation generated cancer fusion proteins and involved in the human neurological diseases amyotrophic lateral sclerosis and fronto-temporal lobar degeneration.ResultsTo determine the gene regulatory functions of FUS and EWS at the level of chromatin, we have performed chromatin immunoprecipitation followed by next generation sequencing (ChIP-seq). Our results show that FUS and EWS bind to a subset of actively transcribed genes, that binding often is downstream the poly(A)-signal, and that binding overlaps with RNA polymerase II. Functional examinations of selected target genes identified that FUS and EWS can regulate gene expression at different levels. Gene Ontology analyses showed that FUS and EWS target genes preferentially encode proteins involved in regulatory processes at the RNA level.ConclusionsThe presented results yield new insights into gene interactions of EWS and FUS and have identified a set of FUS and EWS target genes involved in pathways at the RNA regulatory level with potential to mediate normal and disease-associated functions of the FUS and EWS proteins.",
author = "Yonglun Luo and Friis, {Jenny Blechingberg} and Fernandes, {Ana Miguel} and Shengting Li and Tue Fryland and Anders B{\o}rglum and Lars Bolund and Nielsen, {Anders Lade}",
year = "2015",
month = nov,
language = "English",
volume = "16",
journal = "B M C Genomics",
issn = "1471-2164",
publisher = "BioMed Central Ltd.",

}

RIS

TY - JOUR

T1 - EWS and FUS bind a subset of transcribed genes encoding proteins enriched in RNA regulatory functions

AU - Luo, Yonglun

AU - Friis, Jenny Blechingberg

AU - Fernandes, Ana Miguel

AU - Li, Shengting

AU - Fryland, Tue

AU - Børglum, Anders

AU - Bolund, Lars

AU - Nielsen, Anders Lade

PY - 2015/11

Y1 - 2015/11

N2 - BackgroundFUS (TLS) and EWS (EWSR1) belong to the FET-protein family of RNA and DNA binding proteins. FUS and EWS are structurally and functionally related and participate in transcriptional regulation and RNA processing. FUS and EWS are identified in translocation generated cancer fusion proteins and involved in the human neurological diseases amyotrophic lateral sclerosis and fronto-temporal lobar degeneration.ResultsTo determine the gene regulatory functions of FUS and EWS at the level of chromatin, we have performed chromatin immunoprecipitation followed by next generation sequencing (ChIP-seq). Our results show that FUS and EWS bind to a subset of actively transcribed genes, that binding often is downstream the poly(A)-signal, and that binding overlaps with RNA polymerase II. Functional examinations of selected target genes identified that FUS and EWS can regulate gene expression at different levels. Gene Ontology analyses showed that FUS and EWS target genes preferentially encode proteins involved in regulatory processes at the RNA level.ConclusionsThe presented results yield new insights into gene interactions of EWS and FUS and have identified a set of FUS and EWS target genes involved in pathways at the RNA regulatory level with potential to mediate normal and disease-associated functions of the FUS and EWS proteins.

AB - BackgroundFUS (TLS) and EWS (EWSR1) belong to the FET-protein family of RNA and DNA binding proteins. FUS and EWS are structurally and functionally related and participate in transcriptional regulation and RNA processing. FUS and EWS are identified in translocation generated cancer fusion proteins and involved in the human neurological diseases amyotrophic lateral sclerosis and fronto-temporal lobar degeneration.ResultsTo determine the gene regulatory functions of FUS and EWS at the level of chromatin, we have performed chromatin immunoprecipitation followed by next generation sequencing (ChIP-seq). Our results show that FUS and EWS bind to a subset of actively transcribed genes, that binding often is downstream the poly(A)-signal, and that binding overlaps with RNA polymerase II. Functional examinations of selected target genes identified that FUS and EWS can regulate gene expression at different levels. Gene Ontology analyses showed that FUS and EWS target genes preferentially encode proteins involved in regulatory processes at the RNA level.ConclusionsThe presented results yield new insights into gene interactions of EWS and FUS and have identified a set of FUS and EWS target genes involved in pathways at the RNA regulatory level with potential to mediate normal and disease-associated functions of the FUS and EWS proteins.

M3 - Journal article

VL - 16

JO - B M C Genomics

JF - B M C Genomics

SN - 1471-2164

ER -