Lambert Kristiansen Sørensen

Simultaneous determination of β-hydroxybutyrate and β-hydroxy-β-methylbutyrate in human whole blood using hydrophilic interaction liquid chromatography electrospray tandem mass spectrometry

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Simultaneous determination of β-hydroxybutyrate and β-hydroxy-β-methylbutyrate in human whole blood using hydrophilic interaction liquid chromatography electrospray tandem mass spectrometry. / Sørensen, Lambert K; Rittig, Nikolaj; Holmquist, Emil F et al.

In: Clinical Biochemistry, Vol. 46, 2013, p. 1877-1883.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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@article{4063b0ce5d274db19fb5d94d4db80d6c,
title = "Simultaneous determination of β-hydroxybutyrate and β-hydroxy-β-methylbutyrate in human whole blood using hydrophilic interaction liquid chromatography electrospray tandem mass spectrometry",
abstract = "For the quantification of β-hydroxybutyrate (BHB) and β-hydroxy-β-methylbutyrate (HMB) in human whole blood, a method using hydrophilic interaction liquid chromatography tandem mass spectrometry (HILIC-MS/MS) was developed, which does not require chemical modification of the analytes. Design and methods Samples were deproteinised by a mixture of methanol and acetonitrile, and the extracts were cleaned-up using both polymeric strong cation exchange and strong anion exchange sorbents. The analytes and their structural isomers were separated using a column with a zwitterionic stationary phase. Isotope dilution of both analytes was used for quantitative analysis.",
author = "S{\o}rensen, {Lambert K} and Nikolaj Rittig and Holmquist, {Emil F} and J{\o}rgensen, {Karl Anker} and J{\o}rgensen, {Jens Otto Lunde} and Niels M{\o}ller and Mogens Johannsen",
note = "{\textcopyright} 2013.",
year = "2013",
doi = "10.1016/j.clinbiochem.2013.08.011",
language = "English",
volume = "46",
pages = "1877--1883",
journal = "Clinical Biochemistry",
issn = "0009-9120",
publisher = "Elsevier Inc.",

}

RIS

TY - JOUR

T1 - Simultaneous determination of β-hydroxybutyrate and β-hydroxy-β-methylbutyrate in human whole blood using hydrophilic interaction liquid chromatography electrospray tandem mass spectrometry

AU - Sørensen, Lambert K

AU - Rittig, Nikolaj

AU - Holmquist, Emil F

AU - Jørgensen, Karl Anker

AU - Jørgensen, Jens Otto Lunde

AU - Møller, Niels

AU - Johannsen, Mogens

N1 - © 2013.

PY - 2013

Y1 - 2013

N2 - For the quantification of β-hydroxybutyrate (BHB) and β-hydroxy-β-methylbutyrate (HMB) in human whole blood, a method using hydrophilic interaction liquid chromatography tandem mass spectrometry (HILIC-MS/MS) was developed, which does not require chemical modification of the analytes. Design and methods Samples were deproteinised by a mixture of methanol and acetonitrile, and the extracts were cleaned-up using both polymeric strong cation exchange and strong anion exchange sorbents. The analytes and their structural isomers were separated using a column with a zwitterionic stationary phase. Isotope dilution of both analytes was used for quantitative analysis.

AB - For the quantification of β-hydroxybutyrate (BHB) and β-hydroxy-β-methylbutyrate (HMB) in human whole blood, a method using hydrophilic interaction liquid chromatography tandem mass spectrometry (HILIC-MS/MS) was developed, which does not require chemical modification of the analytes. Design and methods Samples were deproteinised by a mixture of methanol and acetonitrile, and the extracts were cleaned-up using both polymeric strong cation exchange and strong anion exchange sorbents. The analytes and their structural isomers were separated using a column with a zwitterionic stationary phase. Isotope dilution of both analytes was used for quantitative analysis.

U2 - 10.1016/j.clinbiochem.2013.08.011

DO - 10.1016/j.clinbiochem.2013.08.011

M3 - Journal article

C2 - 23994603

VL - 46

SP - 1877

EP - 1883

JO - Clinical Biochemistry

JF - Clinical Biochemistry

SN - 0009-9120

ER -