Kristian Stengaard-Pedersen

SKG arthritis as a model for evaluating therapies in rheumatoid arthritis with special focus on bone changes

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SKG arthritis as a model for evaluating therapies in rheumatoid arthritis with special focus on bone changes. / Keller, Kresten Krarup; Lindgaard, Lisa Mejlvang; Wogensen, Lise; Dagnæs-Hansen, Frederik; Thomsen, Jesper Skovhus; Sakaguchi, Shimon; Stengaard-Pedersen, Kristian; Hauge, Ellen Margrethe.

In: Rheumatology International, Vol. 33, No. 5, 2013, p. 1127-1133.

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@article{1831f7425fbe4a77899e25157102c573,
title = "SKG arthritis as a model for evaluating therapies in rheumatoid arthritis with special focus on bone changes",
abstract = "The aim was to further characterize the SKG model of rheumatoid arthritis (RA) and its potential for studying intervention treatments, with special focus on bone targeting therapies. Three individual studies were conducted, using a total of 71 SKG mice, comparing arthritis induction with mannan versus zymosan A, female versus male mice, and the effect of dexamethasone intervention treatment initiated at different time points after arthritis induction. Hind paws were embedded undecalcified in methyl methacrylate, and sections were stained with Masson-Goldner trichrome. Areal Bone Mineral Density (aBMD) of the femora was determined with pDXA. RNA was extracted from the hind paws followed by the quantification by reverse transcriptase PCR. SKG mice stimulated with mannan presented a higher arthritis score than mice stimulated with zymosan A. Female SKG mice developed a more severe arthritis than male SKG mice. Dexamethasone inhibited arthritis clinically as well as histologically when the treatment was initiated prophylactically or within the first week of arthritis. Femoral aBMD was lower in animals with arthritis than in control animals. The RANKL RNA expression was elevated in arthritic mice, whereas OPG RNA expression was unchanged. The results suggest mannan as arthritis inductor and female instead of male mice in experiments as well as an optimal time window for the initiation of treatment. Systemic bone loss as well as local up regulation of RANKL was present early in SKG arthritis. These results demonstrate that SKG arthritis is a suitable new model for evaluating therapies in RA.",
author = "Keller, {Kresten Krarup} and Lindgaard, {Lisa Mejlvang} and Lise Wogensen and Frederik Dagn{\ae}s-Hansen and Thomsen, {Jesper Skovhus} and Shimon Sakaguchi and Kristian Stengaard-Pedersen and Hauge, {Ellen Margrethe}",
year = "2013",
doi = "10.1007/s00296-012-2500-7",
language = "English",
volume = "33",
pages = "1127--1133",
journal = "Rheumatology International",
issn = "0172-8172",
publisher = "Springer",
number = "5",

}

RIS

TY - JOUR

T1 - SKG arthritis as a model for evaluating therapies in rheumatoid arthritis with special focus on bone changes

AU - Keller, Kresten Krarup

AU - Lindgaard, Lisa Mejlvang

AU - Wogensen, Lise

AU - Dagnæs-Hansen, Frederik

AU - Thomsen, Jesper Skovhus

AU - Sakaguchi, Shimon

AU - Stengaard-Pedersen, Kristian

AU - Hauge, Ellen Margrethe

PY - 2013

Y1 - 2013

N2 - The aim was to further characterize the SKG model of rheumatoid arthritis (RA) and its potential for studying intervention treatments, with special focus on bone targeting therapies. Three individual studies were conducted, using a total of 71 SKG mice, comparing arthritis induction with mannan versus zymosan A, female versus male mice, and the effect of dexamethasone intervention treatment initiated at different time points after arthritis induction. Hind paws were embedded undecalcified in methyl methacrylate, and sections were stained with Masson-Goldner trichrome. Areal Bone Mineral Density (aBMD) of the femora was determined with pDXA. RNA was extracted from the hind paws followed by the quantification by reverse transcriptase PCR. SKG mice stimulated with mannan presented a higher arthritis score than mice stimulated with zymosan A. Female SKG mice developed a more severe arthritis than male SKG mice. Dexamethasone inhibited arthritis clinically as well as histologically when the treatment was initiated prophylactically or within the first week of arthritis. Femoral aBMD was lower in animals with arthritis than in control animals. The RANKL RNA expression was elevated in arthritic mice, whereas OPG RNA expression was unchanged. The results suggest mannan as arthritis inductor and female instead of male mice in experiments as well as an optimal time window for the initiation of treatment. Systemic bone loss as well as local up regulation of RANKL was present early in SKG arthritis. These results demonstrate that SKG arthritis is a suitable new model for evaluating therapies in RA.

AB - The aim was to further characterize the SKG model of rheumatoid arthritis (RA) and its potential for studying intervention treatments, with special focus on bone targeting therapies. Three individual studies were conducted, using a total of 71 SKG mice, comparing arthritis induction with mannan versus zymosan A, female versus male mice, and the effect of dexamethasone intervention treatment initiated at different time points after arthritis induction. Hind paws were embedded undecalcified in methyl methacrylate, and sections were stained with Masson-Goldner trichrome. Areal Bone Mineral Density (aBMD) of the femora was determined with pDXA. RNA was extracted from the hind paws followed by the quantification by reverse transcriptase PCR. SKG mice stimulated with mannan presented a higher arthritis score than mice stimulated with zymosan A. Female SKG mice developed a more severe arthritis than male SKG mice. Dexamethasone inhibited arthritis clinically as well as histologically when the treatment was initiated prophylactically or within the first week of arthritis. Femoral aBMD was lower in animals with arthritis than in control animals. The RANKL RNA expression was elevated in arthritic mice, whereas OPG RNA expression was unchanged. The results suggest mannan as arthritis inductor and female instead of male mice in experiments as well as an optimal time window for the initiation of treatment. Systemic bone loss as well as local up regulation of RANKL was present early in SKG arthritis. These results demonstrate that SKG arthritis is a suitable new model for evaluating therapies in RA.

U2 - 10.1007/s00296-012-2500-7

DO - 10.1007/s00296-012-2500-7

M3 - Journal article

C2 - 22948540

VL - 33

SP - 1127

EP - 1133

JO - Rheumatology International

JF - Rheumatology International

SN - 0172-8172

IS - 5

ER -