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Karen-Marie Pedersen

Characterization of two novel nuclear BTB/POZ domain zinc finger isoforms. Association with differentiation of hippocampal neurons, cerebellar granule cells, and macroglia

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Cathy Mitchelmore
  • ,
  • Karen M Kjaerulff
  • ,
  • Hans C Pedersen
  • ,
  • Jakob V. Nielsen
  • ,
  • Thomas E Rasmussen
  • ,
  • Mads F Fisker
  • ,
  • Bente Finsen
  • ,
  • Karen-Marie Pedersen
  • Niels Aagaard Jensen
BTB/POZ (broad complex tramtrack bric-a-brac/poxvirus and zinc finger) zinc finger factors are a class of nuclear DNA-binding proteins involved in development, chromatin remodeling, and cancer. However, BTB/POZ domain zinc finger factors linked to development of the mammalian cerebral cortex, cerebellum, and macroglia have not been described previously. We report here the isolation and characterization of two novel nuclear BTB/POZ domain zinc finger isoforms, designated HOF(L) and HOF(S), that are specifically expressed in early hippocampal neurons, cerebellar granule cells, and gliogenic progenitors as well as in differentiated glia. During embryonic development of the murine cerebral cortex, HOF expression is restricted to the hippocampal subdivision. Expression coincides with early differentiation of presumptive CA1 and CA3 pyramidal neurons and dentate gyrus granule cells, with a sharp decline in expression at the CA1/subicular border. By using bromodeoxyuridine labeling and immunohistochemistry, we show that HOF expression coincides with immature non-dividing cells and is down-regulated in differentiated cells, suggesting a role for HOF in hippocampal neurogenesis. Consistent with the postulated role of the POZ domain as a site for protein-protein interactions, both HOF isoforms are able to dimerize. The HOF zinc fingers bind specifically to the binding site for the related promyelocytic leukemia zinc finger protein as well as to a newly identified DNA sequence.
Original languageEnglish
JournalJournal of Biological Chemistry
Volume277
Issue9
Pages (from-to)7598-609
Number of pages12
ISSN0021-9258
DOIs
Publication statusPublished - 2002

    Research areas

  • Alternative Splicing, Amino Acid Sequence, Animals, Blotting, Western, Cell Line, Cell Nucleus, Cerebellum, Cerebral Cortex, Cloning, Molecular, DNA, Complementary, Down-Regulation, Gene Library, Glutathione Transferase, Hippocampus, Immunohistochemistry, In Situ Hybridization, Mice, Mice, Transgenic, Microscopy, Fluorescence, Molecular Sequence Data, Neurons, Plasmids, Protein Isoforms, Protein Structure, Tertiary, Recombinant Fusion Proteins, Repressor Proteins, Sequence Homology, Amino Acid, Transcription Factors, Two-Hybrid System Techniques, Zinc Fingers

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