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Jørgen Frøkiær

Tissue, urine and blood metabolite signatures of chronic kidney disease in the 5/6 nephrectomy rat model

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Tissue, urine and blood metabolite signatures of chronic kidney disease in the 5/6 nephrectomy rat model. / Hanifa, Munsoor A.; Skott, Martin; Maltesen, Raluca G.; Rasmussen, Bodil S.; Nielsen, Søren; Frøkiær, Jørgen; Ring, Troels; Wimmer, Reinhard.

In: Metabolomics, Vol. 15, No. 8, 112, 08.2019.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Hanifa, MA, Skott, M, Maltesen, RG, Rasmussen, BS, Nielsen, S, Frøkiær, J, Ring, T & Wimmer, R 2019, 'Tissue, urine and blood metabolite signatures of chronic kidney disease in the 5/6 nephrectomy rat model', Metabolomics, vol. 15, no. 8, 112. https://doi.org/10.1007/s11306-019-1569-3

APA

Hanifa, M. A., Skott, M., Maltesen, R. G., Rasmussen, B. S., Nielsen, S., Frøkiær, J., Ring, T., & Wimmer, R. (2019). Tissue, urine and blood metabolite signatures of chronic kidney disease in the 5/6 nephrectomy rat model. Metabolomics, 15(8), [112]. https://doi.org/10.1007/s11306-019-1569-3

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MLA

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Author

Hanifa, Munsoor A. ; Skott, Martin ; Maltesen, Raluca G. ; Rasmussen, Bodil S. ; Nielsen, Søren ; Frøkiær, Jørgen ; Ring, Troels ; Wimmer, Reinhard. / Tissue, urine and blood metabolite signatures of chronic kidney disease in the 5/6 nephrectomy rat model. In: Metabolomics. 2019 ; Vol. 15, No. 8.

Bibtex

@article{31d50bc9b76f4d719861fe65c6a1458c,
title = "Tissue, urine and blood metabolite signatures of chronic kidney disease in the 5/6 nephrectomy rat model",
abstract = "Introduction: Progressive chronic kidney disease (CKD) is an important cause of morbidity and mortality. It has a long asymptomatic phase, where routine blood tests cannot identify early functional losses, and therefore identifying common mechanisms across the many etiologies is an important goal. Objectives: Our aim was to characterize serum, urine and tissue (kidney, lung, heart, spleen and liver) metabolomics changes in a rat model of CKD. Methods: A total of 17 male Wistar rats underwent 5/6 nephrectomy, whilst 13 rats underwent sham operation. Urine samples were collected weekly, for 6 weeks; blood was collected at weeks 0, 3 and 6; and tissue samples were collected at week 6. Samples were analyzed on a nuclear magnetic resonance spectroscopy platform with multivariate and univariate data analysis. Results: Changes in several metabolites were statistically significant. Allantoin was affected in all compartments. Renal asparagine, creatine, hippurate and trimethylamine were significantly different; in other tissues creatine, dimethylamine, dimethylglycine, trigonelline and trimethylamine were significant. Benzoate, citrate, dimethylglycine, fumarate, guanidinoacetate, malate, myo-inositol and oxoglutarate were altered in urine or serum. Conclusion: Although the metabolic picture is complex, we suggest oxidative stress, the gut-kidney axis, acid–base balance, and energy metabolism as promising areas for future investigation.",
keywords = "5/6 Nephrectomy, Allantoin, Chronic kidney disease, Metabolomics, Oxidative stress",
author = "Hanifa, {Munsoor A.} and Martin Skott and Maltesen, {Raluca G.} and Rasmussen, {Bodil S.} and S{\o}ren Nielsen and J{\o}rgen Fr{\o}ki{\ae}r and Troels Ring and Reinhard Wimmer",
year = "2019",
month = aug,
doi = "10.1007/s11306-019-1569-3",
language = "English",
volume = "15",
journal = "Metabolomics",
issn = "1573-3882",
publisher = "Springer New York LLC",
number = "8",

}

RIS

TY - JOUR

T1 - Tissue, urine and blood metabolite signatures of chronic kidney disease in the 5/6 nephrectomy rat model

AU - Hanifa, Munsoor A.

AU - Skott, Martin

AU - Maltesen, Raluca G.

AU - Rasmussen, Bodil S.

AU - Nielsen, Søren

AU - Frøkiær, Jørgen

AU - Ring, Troels

AU - Wimmer, Reinhard

PY - 2019/8

Y1 - 2019/8

N2 - Introduction: Progressive chronic kidney disease (CKD) is an important cause of morbidity and mortality. It has a long asymptomatic phase, where routine blood tests cannot identify early functional losses, and therefore identifying common mechanisms across the many etiologies is an important goal. Objectives: Our aim was to characterize serum, urine and tissue (kidney, lung, heart, spleen and liver) metabolomics changes in a rat model of CKD. Methods: A total of 17 male Wistar rats underwent 5/6 nephrectomy, whilst 13 rats underwent sham operation. Urine samples were collected weekly, for 6 weeks; blood was collected at weeks 0, 3 and 6; and tissue samples were collected at week 6. Samples were analyzed on a nuclear magnetic resonance spectroscopy platform with multivariate and univariate data analysis. Results: Changes in several metabolites were statistically significant. Allantoin was affected in all compartments. Renal asparagine, creatine, hippurate and trimethylamine were significantly different; in other tissues creatine, dimethylamine, dimethylglycine, trigonelline and trimethylamine were significant. Benzoate, citrate, dimethylglycine, fumarate, guanidinoacetate, malate, myo-inositol and oxoglutarate were altered in urine or serum. Conclusion: Although the metabolic picture is complex, we suggest oxidative stress, the gut-kidney axis, acid–base balance, and energy metabolism as promising areas for future investigation.

AB - Introduction: Progressive chronic kidney disease (CKD) is an important cause of morbidity and mortality. It has a long asymptomatic phase, where routine blood tests cannot identify early functional losses, and therefore identifying common mechanisms across the many etiologies is an important goal. Objectives: Our aim was to characterize serum, urine and tissue (kidney, lung, heart, spleen and liver) metabolomics changes in a rat model of CKD. Methods: A total of 17 male Wistar rats underwent 5/6 nephrectomy, whilst 13 rats underwent sham operation. Urine samples were collected weekly, for 6 weeks; blood was collected at weeks 0, 3 and 6; and tissue samples were collected at week 6. Samples were analyzed on a nuclear magnetic resonance spectroscopy platform with multivariate and univariate data analysis. Results: Changes in several metabolites were statistically significant. Allantoin was affected in all compartments. Renal asparagine, creatine, hippurate and trimethylamine were significantly different; in other tissues creatine, dimethylamine, dimethylglycine, trigonelline and trimethylamine were significant. Benzoate, citrate, dimethylglycine, fumarate, guanidinoacetate, malate, myo-inositol and oxoglutarate were altered in urine or serum. Conclusion: Although the metabolic picture is complex, we suggest oxidative stress, the gut-kidney axis, acid–base balance, and energy metabolism as promising areas for future investigation.

KW - 5/6 Nephrectomy

KW - Allantoin

KW - Chronic kidney disease

KW - Metabolomics

KW - Oxidative stress

UR - http://www.scopus.com/inward/record.url?scp=85070778077&partnerID=8YFLogxK

U2 - 10.1007/s11306-019-1569-3

DO - 10.1007/s11306-019-1569-3

M3 - Journal article

C2 - 31422467

AN - SCOPUS:85070778077

VL - 15

JO - Metabolomics

JF - Metabolomics

SN - 1573-3882

IS - 8

M1 - 112

ER -