Jørgen Frøkiær

α-Melanocyte Stimulating Hormone Treatment in Pigs Does Not Improve Early Graft Function in Kidney Transplants from Brain Dead Donors

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  • Willem G van Rijt, Department of Surgery, University Medical Center Groningen, Groningen, The Netherlands; Department of Pathology and Medical Biology, University Medical Center Groningen, Groningen, The Netherlands.
  • ,
  • Niels Secher
  • Anna K Keller
  • Ulla Møldrup
  • Yahor Chynau, Department of Urology, Aarhus University Hospital, Aarhus, Denmark., Denmark
  • Rutger J Ploeg, Nuffield Department of Surgical Sciences, University of Oxford, Oxford, United Kingdom.
  • ,
  • Harry van Goor, Department of Pathology and Medical Biology, University Medical Center Groningen, Groningen, The Netherlands.
  • ,
  • Rikke Nørregaard
  • Henrik Birn
  • Jørgen Frøkiaer
  • Søren Nielsen, Denmark
  • Henri G D Leuvenink, Department of Surgery, University Medical Center Groningen, Groningen, The Netherlands., Denmark
  • Bente Jespersen

Delayed graft function and primary non-function are serious complications following transplantation of kidneys derived from deceased brain dead (DBD) donors. α-melanocyte stimulating hormone (α-MSH) is a pleiotropic neuropeptide and its renoprotective effects have been demonstrated in models of acute kidney injury. We hypothesized that α-MSH treatment of the recipient improves early graft function and reduces inflammation following DBD kidney transplantation. Eight Danish landrace pigs served as DBD donors. After four hours of brain death both kidneys were removed and stored for 18 hours at 4°C in Custodiol preservation solution. Sixteen recipients were randomized in a paired design into two treatment groups, transplanted simultaneously. α-MSH or a vehicle was administered at start of surgery, during reperfusion and two hours post-reperfusion. The recipients were observed for ten hours following reperfusion. Blood, urine and kidney tissue samples were collected during and at the end of follow-up. α-MSH treatment reduced urine flow and impaired recovery of glomerular filtration rate (GFR) compared to controls. After each dose of α-MSH, a trend towards reduced mean arterial blood pressure and increased heart rate was observed. α-MSH did not affect expression of inflammatory markers. Surprisingly, α-MSH impaired recovery of renal function in the first ten hours following DBD kidney transplantation possibly due to hemodynamic changes. Thus, in a porcine experimental model α-MSH did not reduce renal inflammation and did not improve short-term graft function following DBD kidney transplantation.

Original languageEnglish
JournalPLOS ONE
Volume9
Issue4
Pages (from-to)e94609
ISSN1932-6203
DOIs
Publication statusPublished - 2014

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