Jørgen Frøkiær

Long-Term EGF Treatment Partially Prevents Reduction of Renal Blood Flow in Response to Neonatally Induced Partial Unilateral Ureteral Obstruction

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  • Department of Anatomy
  • Biomedical Radio Isotope Techniques
  • Institute of Clinical Medicine
  • Center of Magnetic Resonance
  • The Department of Urology K
Background/Aims: To investigate the effects of long-term epidermal growth factor (EGF) treatment on kidney growth, renal blood flow (RBF), AQP2 expression and urine output in newborn rats with partial unilateral ureteral obstruction (PUUO). Methods: PUUO (n = 29) and sham-operated control (CON) (n = 26) was created in 2-day-old rats. Fifteen PUUO and 14 CON rats were treated with EGF beginning from the second day after operation to 8 weeks of age. Total kidney volume (TKV) and RBF were measured by MRI at weeks 4 and 8, respectively. Urine volume and osmolality, plasma osmolality, and free water clearance were measured. Renal AQP2 expression was determined using semiquantitative immunoblotting. Results: PUUO induced a severe hydronephrosis and RBF reduction in the obstructed kidney which was attenuated in EGF-treated rats. Body weight, urine output and free water clearance increased, osmolality and the osmolar clearance decreased significantly in EGF-treated rats, despite unchanged AQP2 levels. Conclusions: Long-term EGF treatment attenuated the obstruction- induced RBF reduction. Body weight, urine output increased, and urine osmolality decreased in both control and PUUO rats treated with EGF. Solute-free water clearance increased in PUUO rats which seem to be independent of AQP2 expression.
Original languageEnglish
JournalNephron Experimental Nephrology
Volume111
Issue2
Pages (from-to)e51-e59
ISSN1660-2129
DOIs
Publication statusPublished - 2009

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