Jørgen Frøkiær

Immunolocalization of aquaporin-8 in rat kidney, gastrointestinal tract, testis, and airways

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Immunolocalization of aquaporin-8 in rat kidney, gastrointestinal tract, testis, and airways. / Elkjær, M.-L.; Nejsum, Lene Niemann; Gresz, V et al.

In: American Journal of Physiology: Renal Physiology, Vol. 281, No. 6, 01.12.2001, p. F1047-57.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Elkjær, M-L, Nejsum, LN, Gresz, V, Kwon, TH, Jensen, UB, Frøkiaer, J & Nielsen, S 2001, 'Immunolocalization of aquaporin-8 in rat kidney, gastrointestinal tract, testis, and airways', American Journal of Physiology: Renal Physiology, vol. 281, no. 6, pp. F1047-57.

APA

Elkjær, M-L., Nejsum, L. N., Gresz, V., Kwon, T. H., Jensen, U. B., Frøkiaer, J., & Nielsen, S. (2001). Immunolocalization of aquaporin-8 in rat kidney, gastrointestinal tract, testis, and airways. American Journal of Physiology: Renal Physiology, 281(6), F1047-57.

CBE

Elkjær M-L, Nejsum LN, Gresz V, Kwon TH, Jensen UB, Frøkiaer J, Nielsen S. 2001. Immunolocalization of aquaporin-8 in rat kidney, gastrointestinal tract, testis, and airways. American Journal of Physiology: Renal Physiology. 281(6):F1047-57.

MLA

Elkjær, M.-L. et al. "Immunolocalization of aquaporin-8 in rat kidney, gastrointestinal tract, testis, and airways". American Journal of Physiology: Renal Physiology. 2001, 281(6). F1047-57.

Vancouver

Elkjær M-L, Nejsum LN, Gresz V, Kwon TH, Jensen UB, Frøkiaer J et al. Immunolocalization of aquaporin-8 in rat kidney, gastrointestinal tract, testis, and airways. American Journal of Physiology: Renal Physiology. 2001 Dec 1;281(6):F1047-57.

Author

Elkjær, M.-L. ; Nejsum, Lene Niemann ; Gresz, V et al. / Immunolocalization of aquaporin-8 in rat kidney, gastrointestinal tract, testis, and airways. In: American Journal of Physiology: Renal Physiology. 2001 ; Vol. 281, No. 6. pp. F1047-57.

Bibtex

@article{90ff7e2e7f1b46c7abb831de16f5ea6e,
title = "Immunolocalization of aquaporin-8 in rat kidney, gastrointestinal tract, testis, and airways",
abstract = "The purpose of this study was to determine the cellular and subcellular localization of aquaporin-8 (AQP8) in rat kidney and other organs by RT-PCR analyses and by immunoblotting and immunohistochemistry using peptide-derived rabbit antibodies to rat AQP8. RT-PCR and Southern blotting revealed the presence of AQP8 mRNA in all kidney zones. LLC-PK(1) cells transfected with a rat AQP8 construct exhibited strong labeling with the affinity-purified antibodies, whereas controls using cells transfected with the vector, but without the insert, were negative. The labeling was almost exclusively associated with intracellular vesicles. Immunoblotting of kidney membrane fractions revealed a predominant single band of 26-28 kDa. AQP8 immunoreactivity was mainly present in the cortex and outer stripe of the outer medulla. Sequential ultracentrifugation of rat kidney membrane revealed that AQP8 resides predominantly in intracellular vesicular fractions. Immunocytochemistry revealed modest labeling of proximal tubules and weak labeling of collecting ducts in cortex and medulla of rat kidney. The labeling was confined to cytoplasmic areas with no labeling of the brush border. Immunoblotting and RT-PCR/Southern blotting also revealed the presence of AQP8 protein and mRNA in rat liver, testis, epididymis, duodenum, jejunum, colon, and bronchi/trachea. Consistent with this, immunohistochemistry revealed AQP8 labeling in the hepatocytes and spermatogenic cells in testis and in the basal cells in ductus epididymis, trachea, and bronchial epithelia. Moreover, AQP8 labeling was observed in the myoepithelial cells in salivary, bronchial, and tracheal glands with no labeling of acini or ductal epithelial cells. AQP8 is also present in the surface epithelial cells in duodenum, jejunum, and colon. In conclusion, AQP8 is expressed at low levels in rat kidney proximal tubules and collecting ducts, and it is present in distinct cell types in liver, testis, epididymis, duodenum, jejunum, colon, trachea, and principal bronchi as well as in multiple glands, including salivary glands.",
keywords = "Animals, Aquaporins, Cell Line, Digestive System, Epididymis, Immunoblotting, Immunohistochemistry, Intestinal Mucosa, Ion Channels, Kidney, Liver, Male, RNA, Messenger, Rats, Rats, Wistar, Respiratory Mucosa, Respiratory System, Salivary Glands, Testis, Tissue Distribution, Transfection",
author = "M.-L. Elkj{\ae}r and Nejsum, {Lene Niemann} and V Gresz and Kwon, {T H} and Jensen, {U B} and J Fr{\o}kiaer and S{\o}ren Nielsen",
year = "2001",
month = dec,
day = "1",
language = "English",
volume = "281",
pages = "F1047--57",
journal = "American Journal of Physiology: Renal Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "6",

}

RIS

TY - JOUR

T1 - Immunolocalization of aquaporin-8 in rat kidney, gastrointestinal tract, testis, and airways

AU - Elkjær, M.-L.

AU - Nejsum, Lene Niemann

AU - Gresz, V

AU - Kwon, T H

AU - Jensen, U B

AU - Frøkiaer, J

AU - Nielsen, Søren

PY - 2001/12/1

Y1 - 2001/12/1

N2 - The purpose of this study was to determine the cellular and subcellular localization of aquaporin-8 (AQP8) in rat kidney and other organs by RT-PCR analyses and by immunoblotting and immunohistochemistry using peptide-derived rabbit antibodies to rat AQP8. RT-PCR and Southern blotting revealed the presence of AQP8 mRNA in all kidney zones. LLC-PK(1) cells transfected with a rat AQP8 construct exhibited strong labeling with the affinity-purified antibodies, whereas controls using cells transfected with the vector, but without the insert, were negative. The labeling was almost exclusively associated with intracellular vesicles. Immunoblotting of kidney membrane fractions revealed a predominant single band of 26-28 kDa. AQP8 immunoreactivity was mainly present in the cortex and outer stripe of the outer medulla. Sequential ultracentrifugation of rat kidney membrane revealed that AQP8 resides predominantly in intracellular vesicular fractions. Immunocytochemistry revealed modest labeling of proximal tubules and weak labeling of collecting ducts in cortex and medulla of rat kidney. The labeling was confined to cytoplasmic areas with no labeling of the brush border. Immunoblotting and RT-PCR/Southern blotting also revealed the presence of AQP8 protein and mRNA in rat liver, testis, epididymis, duodenum, jejunum, colon, and bronchi/trachea. Consistent with this, immunohistochemistry revealed AQP8 labeling in the hepatocytes and spermatogenic cells in testis and in the basal cells in ductus epididymis, trachea, and bronchial epithelia. Moreover, AQP8 labeling was observed in the myoepithelial cells in salivary, bronchial, and tracheal glands with no labeling of acini or ductal epithelial cells. AQP8 is also present in the surface epithelial cells in duodenum, jejunum, and colon. In conclusion, AQP8 is expressed at low levels in rat kidney proximal tubules and collecting ducts, and it is present in distinct cell types in liver, testis, epididymis, duodenum, jejunum, colon, trachea, and principal bronchi as well as in multiple glands, including salivary glands.

AB - The purpose of this study was to determine the cellular and subcellular localization of aquaporin-8 (AQP8) in rat kidney and other organs by RT-PCR analyses and by immunoblotting and immunohistochemistry using peptide-derived rabbit antibodies to rat AQP8. RT-PCR and Southern blotting revealed the presence of AQP8 mRNA in all kidney zones. LLC-PK(1) cells transfected with a rat AQP8 construct exhibited strong labeling with the affinity-purified antibodies, whereas controls using cells transfected with the vector, but without the insert, were negative. The labeling was almost exclusively associated with intracellular vesicles. Immunoblotting of kidney membrane fractions revealed a predominant single band of 26-28 kDa. AQP8 immunoreactivity was mainly present in the cortex and outer stripe of the outer medulla. Sequential ultracentrifugation of rat kidney membrane revealed that AQP8 resides predominantly in intracellular vesicular fractions. Immunocytochemistry revealed modest labeling of proximal tubules and weak labeling of collecting ducts in cortex and medulla of rat kidney. The labeling was confined to cytoplasmic areas with no labeling of the brush border. Immunoblotting and RT-PCR/Southern blotting also revealed the presence of AQP8 protein and mRNA in rat liver, testis, epididymis, duodenum, jejunum, colon, and bronchi/trachea. Consistent with this, immunohistochemistry revealed AQP8 labeling in the hepatocytes and spermatogenic cells in testis and in the basal cells in ductus epididymis, trachea, and bronchial epithelia. Moreover, AQP8 labeling was observed in the myoepithelial cells in salivary, bronchial, and tracheal glands with no labeling of acini or ductal epithelial cells. AQP8 is also present in the surface epithelial cells in duodenum, jejunum, and colon. In conclusion, AQP8 is expressed at low levels in rat kidney proximal tubules and collecting ducts, and it is present in distinct cell types in liver, testis, epididymis, duodenum, jejunum, colon, trachea, and principal bronchi as well as in multiple glands, including salivary glands.

KW - Animals

KW - Aquaporins

KW - Cell Line

KW - Digestive System

KW - Epididymis

KW - Immunoblotting

KW - Immunohistochemistry

KW - Intestinal Mucosa

KW - Ion Channels

KW - Kidney

KW - Liver

KW - Male

KW - RNA, Messenger

KW - Rats

KW - Rats, Wistar

KW - Respiratory Mucosa

KW - Respiratory System

KW - Salivary Glands

KW - Testis

KW - Tissue Distribution

KW - Transfection

M3 - Journal article

C2 - 11704555

VL - 281

SP - F1047-57

JO - American Journal of Physiology: Renal Physiology

JF - American Journal of Physiology: Renal Physiology

SN - 1931-857X

IS - 6

ER -