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Jørgen Frøkiær

Cyclooxygenase 2 inhibition exacerbates AQP2 and pAQP2 downregulation independently of V2 receptor abundance in the postobstructed kidney

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Cyclooxygenase 2 inhibition exacerbates AQP2 and pAQP2 downregulation independently of V2 receptor abundance in the postobstructed kidney. / Jensen, Anja M; Bae, Eun Hui; Nørregaard, Rikke; Wang, Guixian; Nielsen, Søren; Schweer, Horst; Kim, Soo Wan; Frøkiaer, Jørgen.

In: American Journal of Physiology: Renal Physiology, Vol. 298, No. 4, 2010, p. F941-50.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Jensen, AM, Bae, EH, Nørregaard, R, Wang, G, Nielsen, S, Schweer, H, Kim, SW & Frøkiaer, J 2010, 'Cyclooxygenase 2 inhibition exacerbates AQP2 and pAQP2 downregulation independently of V2 receptor abundance in the postobstructed kidney', American Journal of Physiology: Renal Physiology, vol. 298, no. 4, pp. F941-50. https://doi.org/10.1152/ajprenal.00605.2009

APA

Jensen, A. M., Bae, E. H., Nørregaard, R., Wang, G., Nielsen, S., Schweer, H., Kim, S. W., & Frøkiaer, J. (2010). Cyclooxygenase 2 inhibition exacerbates AQP2 and pAQP2 downregulation independently of V2 receptor abundance in the postobstructed kidney. American Journal of Physiology: Renal Physiology, 298(4), F941-50. https://doi.org/10.1152/ajprenal.00605.2009

CBE

MLA

Vancouver

Author

Jensen, Anja M ; Bae, Eun Hui ; Nørregaard, Rikke ; Wang, Guixian ; Nielsen, Søren ; Schweer, Horst ; Kim, Soo Wan ; Frøkiaer, Jørgen. / Cyclooxygenase 2 inhibition exacerbates AQP2 and pAQP2 downregulation independently of V2 receptor abundance in the postobstructed kidney. In: American Journal of Physiology: Renal Physiology. 2010 ; Vol. 298, No. 4. pp. F941-50.

Bibtex

@article{5d093fe0af6111df8c1a000ea68e967b,
title = "Cyclooxygenase 2 inhibition exacerbates AQP2 and pAQP2 downregulation independently of V2 receptor abundance in the postobstructed kidney",
abstract = "Previously we demonstrated that ANG II receptor (AT1R) blockade attenuates V2 receptor (V2R), AQP2, and pS256-AQP2 downregulation in the postobstructed kidney and partially reverses obstruction-induced inhibition of cAMP generation and cyclooxygenase 2 (COX-2) induction. Therefore, we speculated whether the effects of AT1R blockade on V2R and the vasopressin-regulated pathway are attributable to attenuated COX-2 induction. To examine this, rats were subjected to 24-h bilateral ureteral obstruction (BUO) followed by 48-h release and treated with the COX-2 inhibitor parecoxib or saline. Control rats were sham-operated. Parecoxib treatment significantly reduced urine output 24 h after release of BUO whereas urine osmolality and solute-free water reabsorption was comparable between saline- and parecoxib-treated BUO rats. Immunoblotting revealed a significant decrease in AQP2 and pS256-AQP2 abundance to 20 and 23% of sham levels in parecoxib-treated BUO rats compared with 40 and 55% of sham levels in saline-treated BUO rats. Immunohistochemistry confirmed the exacerbated AQP2 and pS256-AQP2 downregulation in parecoxib-treated BUO rats. Finally, parecoxib treatment had no effect on V2R downregulation and the inhibited, vasopressin-stimulated cAMP generation in inner medullary membrane fractions from the postobstructed kidney. In conclusion, COX-2 inhibition exacerbates AQP2 and pS256-AQP2 downregulation 48 h after release of 24-h BUO independently of V2R abundance and vasopressin-stimulated cAMP generation. The results indicate that COX-2 inhibition does not mimic AT1R blockade-mediated effects and that AT1R-mediated AQP2 regulation in the postobstructed kidney collecting duct is independent of COX-2 induction.",
keywords = "Adenylate Cyclase, Animals, Aquaporin 2, Cyclooxygenase 2 Inhibitors, Down-Regulation, Isoxazoles, Kidney, Male, Phosphorylation, Rats, Rats, Sprague-Dawley, Receptors, Vasopressin, Reperfusion Injury, Sodium-Potassium-Chloride Symporters, Vasopressins",
author = "Jensen, {Anja M} and Bae, {Eun Hui} and Rikke N{\o}rregaard and Guixian Wang and S{\o}ren Nielsen and Horst Schweer and Kim, {Soo Wan} and J{\o}rgen Fr{\o}kiaer",
year = "2010",
doi = "10.1152/ajprenal.00605.2009",
language = "English",
volume = "298",
pages = "F941--50",
journal = "American Journal of Physiology: Renal Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "4",

}

RIS

TY - JOUR

T1 - Cyclooxygenase 2 inhibition exacerbates AQP2 and pAQP2 downregulation independently of V2 receptor abundance in the postobstructed kidney

AU - Jensen, Anja M

AU - Bae, Eun Hui

AU - Nørregaard, Rikke

AU - Wang, Guixian

AU - Nielsen, Søren

AU - Schweer, Horst

AU - Kim, Soo Wan

AU - Frøkiaer, Jørgen

PY - 2010

Y1 - 2010

N2 - Previously we demonstrated that ANG II receptor (AT1R) blockade attenuates V2 receptor (V2R), AQP2, and pS256-AQP2 downregulation in the postobstructed kidney and partially reverses obstruction-induced inhibition of cAMP generation and cyclooxygenase 2 (COX-2) induction. Therefore, we speculated whether the effects of AT1R blockade on V2R and the vasopressin-regulated pathway are attributable to attenuated COX-2 induction. To examine this, rats were subjected to 24-h bilateral ureteral obstruction (BUO) followed by 48-h release and treated with the COX-2 inhibitor parecoxib or saline. Control rats were sham-operated. Parecoxib treatment significantly reduced urine output 24 h after release of BUO whereas urine osmolality and solute-free water reabsorption was comparable between saline- and parecoxib-treated BUO rats. Immunoblotting revealed a significant decrease in AQP2 and pS256-AQP2 abundance to 20 and 23% of sham levels in parecoxib-treated BUO rats compared with 40 and 55% of sham levels in saline-treated BUO rats. Immunohistochemistry confirmed the exacerbated AQP2 and pS256-AQP2 downregulation in parecoxib-treated BUO rats. Finally, parecoxib treatment had no effect on V2R downregulation and the inhibited, vasopressin-stimulated cAMP generation in inner medullary membrane fractions from the postobstructed kidney. In conclusion, COX-2 inhibition exacerbates AQP2 and pS256-AQP2 downregulation 48 h after release of 24-h BUO independently of V2R abundance and vasopressin-stimulated cAMP generation. The results indicate that COX-2 inhibition does not mimic AT1R blockade-mediated effects and that AT1R-mediated AQP2 regulation in the postobstructed kidney collecting duct is independent of COX-2 induction.

AB - Previously we demonstrated that ANG II receptor (AT1R) blockade attenuates V2 receptor (V2R), AQP2, and pS256-AQP2 downregulation in the postobstructed kidney and partially reverses obstruction-induced inhibition of cAMP generation and cyclooxygenase 2 (COX-2) induction. Therefore, we speculated whether the effects of AT1R blockade on V2R and the vasopressin-regulated pathway are attributable to attenuated COX-2 induction. To examine this, rats were subjected to 24-h bilateral ureteral obstruction (BUO) followed by 48-h release and treated with the COX-2 inhibitor parecoxib or saline. Control rats were sham-operated. Parecoxib treatment significantly reduced urine output 24 h after release of BUO whereas urine osmolality and solute-free water reabsorption was comparable between saline- and parecoxib-treated BUO rats. Immunoblotting revealed a significant decrease in AQP2 and pS256-AQP2 abundance to 20 and 23% of sham levels in parecoxib-treated BUO rats compared with 40 and 55% of sham levels in saline-treated BUO rats. Immunohistochemistry confirmed the exacerbated AQP2 and pS256-AQP2 downregulation in parecoxib-treated BUO rats. Finally, parecoxib treatment had no effect on V2R downregulation and the inhibited, vasopressin-stimulated cAMP generation in inner medullary membrane fractions from the postobstructed kidney. In conclusion, COX-2 inhibition exacerbates AQP2 and pS256-AQP2 downregulation 48 h after release of 24-h BUO independently of V2R abundance and vasopressin-stimulated cAMP generation. The results indicate that COX-2 inhibition does not mimic AT1R blockade-mediated effects and that AT1R-mediated AQP2 regulation in the postobstructed kidney collecting duct is independent of COX-2 induction.

KW - Adenylate Cyclase

KW - Animals

KW - Aquaporin 2

KW - Cyclooxygenase 2 Inhibitors

KW - Down-Regulation

KW - Isoxazoles

KW - Kidney

KW - Male

KW - Phosphorylation

KW - Rats

KW - Rats, Sprague-Dawley

KW - Receptors, Vasopressin

KW - Reperfusion Injury

KW - Sodium-Potassium-Chloride Symporters

KW - Vasopressins

U2 - 10.1152/ajprenal.00605.2009

DO - 10.1152/ajprenal.00605.2009

M3 - Journal article

C2 - 20107111

VL - 298

SP - F941-50

JO - American Journal of Physiology: Renal Physiology

JF - American Journal of Physiology: Renal Physiology

SN - 1931-857X

IS - 4

ER -