Jørgen Frøkiær

Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra. / Hanifa, Munsoor Ali; Maltesen, Raluca G.; Rasmussen, Bodil S.; Buggeskov, Katrine B.; Ravn, Hanne Berg; Skott, Martin; Nielsen, Søren; Frøkiær, Jørgen; Ring, Troels; Wimmer, Reinhard.

In: Metabolomics, Vol. 16, No. 1, 7, 01.2020.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Hanifa, MA, Maltesen, RG, Rasmussen, BS, Buggeskov, KB, Ravn, HB, Skott, M, Nielsen, S, Frøkiær, J, Ring, T & Wimmer, R 2020, 'Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra', Metabolomics, vol. 16, no. 1, 7. https://doi.org/10.1007/s11306-019-1629-8

APA

Hanifa, M. A., Maltesen, R. G., Rasmussen, B. S., Buggeskov, K. B., Ravn, H. B., Skott, M., Nielsen, S., Frøkiær, J., Ring, T., & Wimmer, R. (2020). Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra. Metabolomics, 16(1), [7]. https://doi.org/10.1007/s11306-019-1629-8

CBE

Hanifa MA, Maltesen RG, Rasmussen BS, Buggeskov KB, Ravn HB, Skott M, Nielsen S, Frøkiær J, Ring T, Wimmer R. 2020. Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra. Metabolomics. 16(1):Article 7. https://doi.org/10.1007/s11306-019-1629-8

MLA

Vancouver

Hanifa MA, Maltesen RG, Rasmussen BS, Buggeskov KB, Ravn HB, Skott M et al. Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra. Metabolomics. 2020 Jan;16(1). 7. https://doi.org/10.1007/s11306-019-1629-8

Author

Hanifa, Munsoor Ali ; Maltesen, Raluca G. ; Rasmussen, Bodil S. ; Buggeskov, Katrine B. ; Ravn, Hanne Berg ; Skott, Martin ; Nielsen, Søren ; Frøkiær, Jørgen ; Ring, Troels ; Wimmer, Reinhard. / Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra. In: Metabolomics. 2020 ; Vol. 16, No. 1.

Bibtex

@article{0ee2a960850a4b988b3bc2176e21b534,
title = "Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra",
abstract = "{\textcopyright} 2019, Springer Science+Business Media, LLC, part of Springer Nature. Background: In our metabolomics studies we have noticed that repeated NMR acquisition on the same sample can result in altered metabolite signal intensities. Aims: To investigate the reproducibility of repeated NMR acquisition on selected metabolites in serum and plasma from two large human metabolomics studies. Methods: Two peak regions for each metabolite were integrated and changes occurring after reacquisition were correlated. Results: Integral changes were generally small, but serum citrate signals decreased significantly in some samples. Conclusions: Several metabolite integrals were not reproducible in some of the repeated spectra. Following established protocols, randomising analysis order and biomarker validation are important.",
keywords = "Metabolomics, NMR, Plasma, Reproducibility, Serum",
author = "Hanifa, {Munsoor Ali} and Maltesen, {Raluca G.} and Rasmussen, {Bodil S.} and Buggeskov, {Katrine B.} and Ravn, {Hanne Berg} and Martin Skott and S{\o}ren Nielsen and J{\o}rgen Fr{\o}ki{\ae}r and Troels Ring and Reinhard Wimmer",
year = "2020",
month = jan,
doi = "10.1007/s11306-019-1629-8",
language = "English",
volume = "16",
journal = "Metabolomics",
issn = "1573-3882",
publisher = "Springer New York LLC",
number = "1",

}

RIS

TY - JOUR

T1 - Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra

AU - Hanifa, Munsoor Ali

AU - Maltesen, Raluca G.

AU - Rasmussen, Bodil S.

AU - Buggeskov, Katrine B.

AU - Ravn, Hanne Berg

AU - Skott, Martin

AU - Nielsen, Søren

AU - Frøkiær, Jørgen

AU - Ring, Troels

AU - Wimmer, Reinhard

PY - 2020/1

Y1 - 2020/1

N2 - © 2019, Springer Science+Business Media, LLC, part of Springer Nature. Background: In our metabolomics studies we have noticed that repeated NMR acquisition on the same sample can result in altered metabolite signal intensities. Aims: To investigate the reproducibility of repeated NMR acquisition on selected metabolites in serum and plasma from two large human metabolomics studies. Methods: Two peak regions for each metabolite were integrated and changes occurring after reacquisition were correlated. Results: Integral changes were generally small, but serum citrate signals decreased significantly in some samples. Conclusions: Several metabolite integrals were not reproducible in some of the repeated spectra. Following established protocols, randomising analysis order and biomarker validation are important.

AB - © 2019, Springer Science+Business Media, LLC, part of Springer Nature. Background: In our metabolomics studies we have noticed that repeated NMR acquisition on the same sample can result in altered metabolite signal intensities. Aims: To investigate the reproducibility of repeated NMR acquisition on selected metabolites in serum and plasma from two large human metabolomics studies. Methods: Two peak regions for each metabolite were integrated and changes occurring after reacquisition were correlated. Results: Integral changes were generally small, but serum citrate signals decreased significantly in some samples. Conclusions: Several metabolite integrals were not reproducible in some of the repeated spectra. Following established protocols, randomising analysis order and biomarker validation are important.

KW - Metabolomics

KW - NMR

KW - Plasma

KW - Reproducibility

KW - Serum

UR - http://www.scopus.com/inward/record.url?scp=85076909285&partnerID=8YFLogxK

U2 - 10.1007/s11306-019-1629-8

DO - 10.1007/s11306-019-1629-8

M3 - Journal article

C2 - 31858270

AN - SCOPUS:85076909285

VL - 16

JO - Metabolomics

JF - Metabolomics

SN - 1573-3882

IS - 1

M1 - 7

ER -