Jørgen Frøkiær
Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra
Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review
- Munsoor Ali Hanifa, Aalborg University, Denmark
- Raluca G. Maltesen, Aalborg University
, - Bodil S. Rasmussen
, - Katrine B. Buggeskov, University of Copenhagen
, - Hanne Berg Ravn, University of Copenhagen, Denmark
- Martin Skott
- Søren Nielsen, Meta-IQ, Denmark
- Jørgen Frøkiær
- Troels Ring, University of Pittsburgh
, - Reinhard Wimmer, Aalborg University
© 2019, Springer Science+Business Media, LLC, part of Springer Nature. Background: In our metabolomics studies we have noticed that repeated NMR acquisition on the same sample can result in altered metabolite signal intensities. Aims: To investigate the reproducibility of repeated NMR acquisition on selected metabolites in serum and plasma from two large human metabolomics studies. Methods: Two peak regions for each metabolite were integrated and changes occurring after reacquisition were correlated. Results: Integral changes were generally small, but serum citrate signals decreased significantly in some samples. Conclusions: Several metabolite integrals were not reproducible in some of the repeated spectra. Following established protocols, randomising analysis order and biomarker validation are important.
| Original language | English |
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| Article number | 7 |
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| Journal | Metabolomics |
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| Volume | 16 |
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| Issue | 1 |
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| Number of pages | 7 |
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| ISSN | 1573-3882 |
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| DOIs | |
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| Publication status | Published - Jan 2020 |
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- Metabolomics, NMR, Plasma, Reproducibility, Serum
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ID: 186086648