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Jørgen Frøkiær

Cisplatin decreases renal cyclooxygenase-2 expression and activity in rats

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Cisplatin decreases renal cyclooxygenase-2 expression and activity in rats. / Tusgaard, Birgitte; Nørregaard, Rikke; Jensen, Anja M; Wang, Guixian; Topcu, Sükrü O; Wang, Yan; Nielsen, Søren; Frøkiaer, Jørgen.

In: Acta Physiologica (Print), Vol. 202, No. 1, 27.01.2011, p. 79-90.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Tusgaard, B, Nørregaard, R, Jensen, AM, Wang, G, Topcu, SO, Wang, Y, Nielsen, S & Frøkiaer, J 2011, 'Cisplatin decreases renal cyclooxygenase-2 expression and activity in rats', Acta Physiologica (Print), vol. 202, no. 1, pp. 79-90. https://doi.org/10.1111/j.1748-1716.2011.02257.x

APA

Tusgaard, B., Nørregaard, R., Jensen, A. M., Wang, G., Topcu, S. O., Wang, Y., Nielsen, S., & Frøkiaer, J. (2011). Cisplatin decreases renal cyclooxygenase-2 expression and activity in rats. Acta Physiologica (Print), 202(1), 79-90. https://doi.org/10.1111/j.1748-1716.2011.02257.x

CBE

Tusgaard B, Nørregaard R, Jensen AM, Wang G, Topcu SO, Wang Y, Nielsen S, Frøkiaer J. 2011. Cisplatin decreases renal cyclooxygenase-2 expression and activity in rats. Acta Physiologica (Print). 202(1):79-90. https://doi.org/10.1111/j.1748-1716.2011.02257.x

MLA

Vancouver

Tusgaard B, Nørregaard R, Jensen AM, Wang G, Topcu SO, Wang Y et al. Cisplatin decreases renal cyclooxygenase-2 expression and activity in rats. Acta Physiologica (Print). 2011 Jan 27;202(1):79-90. https://doi.org/10.1111/j.1748-1716.2011.02257.x

Author

Tusgaard, Birgitte ; Nørregaard, Rikke ; Jensen, Anja M ; Wang, Guixian ; Topcu, Sükrü O ; Wang, Yan ; Nielsen, Søren ; Frøkiaer, Jørgen. / Cisplatin decreases renal cyclooxygenase-2 expression and activity in rats. In: Acta Physiologica (Print). 2011 ; Vol. 202, No. 1. pp. 79-90.

Bibtex

@article{e87be1994dee4437a72bf7bc6977ea93,
title = "Cisplatin decreases renal cyclooxygenase-2 expression and activity in rats",
abstract = "Aim:  Cisplatin induced acute renal failure has previously been associated with decreased urinary prostaglandin E2 excretion and reduced aquaporin 2 expression in kidney collecting duct. In this study we examined the expression of cyclooxygenase-1 and 2 as well as aquaporin 2 and the Na-K-2Cl cotransporter in kidneys from rats with cisplatin induced acute renal failure. Methods:  Rats were treated with either cisplatin or saline and followed for 5 days. Kidneys were dissected into 3 zones and prepared for immunoblotting, quantitative PCR and immunohistochemistry. Renal content and urinary prostaglandin E2 excretion was measured. Results:  Cisplatin treatment was associated with polyuria and a significant decreased creatinine clearence. Inner medullary prostaglandin E2 content and urinary prostaglandin E2 excretion was decreased in cisplatin treated rats. Quantitative polymerase chain reaction and semiquatitative immunoblotting demonstrated that cisplatin treatment reduced cyclooxygenase-2, aquaporin 2 and Na-K-2Cl cotransporter abundance in the different kidney zones, whereas no change in cyclooxygenase-1 was observed. Results were confirmed by immunohistochemistry. Conclusion:  Cyclooxygenase-2 expression is decreased in inner medulla and cortex. Consistent with this urinary prostaglandin E2 levels were reduced. These data suggest that downregulation of cyclooxygenase-2 is responsible for impaired de novo generation of vasodilatory prostaglandins which may play an important role for the cisplatin induced renal vasoconstriction and development of nephropathy.",
author = "Birgitte Tusgaard and Rikke N{\o}rregaard and Jensen, {Anja M} and Guixian Wang and Topcu, {S{\"u}kr{\"u} O} and Yan Wang and S{\o}ren Nielsen and J{\o}rgen Fr{\o}kiaer",
note = "Copyright {\textcopyright} 2011 Scandinavian Physiological Society.",
year = "2011",
month = jan,
day = "27",
doi = "10.1111/j.1748-1716.2011.02257.x",
language = "English",
volume = "202",
pages = "79--90",
journal = "Acta Physiologica (Print)",
issn = "1748-1708",
publisher = "Wiley-Blackwell Publishing Ltd.",
number = "1",

}

RIS

TY - JOUR

T1 - Cisplatin decreases renal cyclooxygenase-2 expression and activity in rats

AU - Tusgaard, Birgitte

AU - Nørregaard, Rikke

AU - Jensen, Anja M

AU - Wang, Guixian

AU - Topcu, Sükrü O

AU - Wang, Yan

AU - Nielsen, Søren

AU - Frøkiaer, Jørgen

N1 - Copyright © 2011 Scandinavian Physiological Society.

PY - 2011/1/27

Y1 - 2011/1/27

N2 - Aim:  Cisplatin induced acute renal failure has previously been associated with decreased urinary prostaglandin E2 excretion and reduced aquaporin 2 expression in kidney collecting duct. In this study we examined the expression of cyclooxygenase-1 and 2 as well as aquaporin 2 and the Na-K-2Cl cotransporter in kidneys from rats with cisplatin induced acute renal failure. Methods:  Rats were treated with either cisplatin or saline and followed for 5 days. Kidneys were dissected into 3 zones and prepared for immunoblotting, quantitative PCR and immunohistochemistry. Renal content and urinary prostaglandin E2 excretion was measured. Results:  Cisplatin treatment was associated with polyuria and a significant decreased creatinine clearence. Inner medullary prostaglandin E2 content and urinary prostaglandin E2 excretion was decreased in cisplatin treated rats. Quantitative polymerase chain reaction and semiquatitative immunoblotting demonstrated that cisplatin treatment reduced cyclooxygenase-2, aquaporin 2 and Na-K-2Cl cotransporter abundance in the different kidney zones, whereas no change in cyclooxygenase-1 was observed. Results were confirmed by immunohistochemistry. Conclusion:  Cyclooxygenase-2 expression is decreased in inner medulla and cortex. Consistent with this urinary prostaglandin E2 levels were reduced. These data suggest that downregulation of cyclooxygenase-2 is responsible for impaired de novo generation of vasodilatory prostaglandins which may play an important role for the cisplatin induced renal vasoconstriction and development of nephropathy.

AB - Aim:  Cisplatin induced acute renal failure has previously been associated with decreased urinary prostaglandin E2 excretion and reduced aquaporin 2 expression in kidney collecting duct. In this study we examined the expression of cyclooxygenase-1 and 2 as well as aquaporin 2 and the Na-K-2Cl cotransporter in kidneys from rats with cisplatin induced acute renal failure. Methods:  Rats were treated with either cisplatin or saline and followed for 5 days. Kidneys were dissected into 3 zones and prepared for immunoblotting, quantitative PCR and immunohistochemistry. Renal content and urinary prostaglandin E2 excretion was measured. Results:  Cisplatin treatment was associated with polyuria and a significant decreased creatinine clearence. Inner medullary prostaglandin E2 content and urinary prostaglandin E2 excretion was decreased in cisplatin treated rats. Quantitative polymerase chain reaction and semiquatitative immunoblotting demonstrated that cisplatin treatment reduced cyclooxygenase-2, aquaporin 2 and Na-K-2Cl cotransporter abundance in the different kidney zones, whereas no change in cyclooxygenase-1 was observed. Results were confirmed by immunohistochemistry. Conclusion:  Cyclooxygenase-2 expression is decreased in inner medulla and cortex. Consistent with this urinary prostaglandin E2 levels were reduced. These data suggest that downregulation of cyclooxygenase-2 is responsible for impaired de novo generation of vasodilatory prostaglandins which may play an important role for the cisplatin induced renal vasoconstriction and development of nephropathy.

U2 - 10.1111/j.1748-1716.2011.02257.x

DO - 10.1111/j.1748-1716.2011.02257.x

M3 - Journal article

C2 - 21272267

VL - 202

SP - 79

EP - 90

JO - Acta Physiologica (Print)

JF - Acta Physiologica (Print)

SN - 1748-1708

IS - 1

ER -