Jørgen Frøkiær

Changes in cellular composition of kidney collecting duct cells in rats with lithium-induced NDI

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Changes in cellular composition of kidney collecting duct cells in rats with lithium-induced NDI. / Christensen, Birgitte Mønster; Marples, David; Kim, Young-Hee; Wang, Weidong; Frøkiaer, Jørgen; Nielsen, Søren.

In: American Journal of Physiology: Cell Physiology, Vol. 286, No. 4, 2004, p. C952-64.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Christensen, BM, Marples, D, Kim, Y-H, Wang, W, Frøkiaer, J & Nielsen, S 2004, 'Changes in cellular composition of kidney collecting duct cells in rats with lithium-induced NDI', American Journal of Physiology: Cell Physiology, vol. 286, no. 4, pp. C952-64. https://doi.org/10.1152/ajpcell.00266.2003

APA

Christensen, B. M., Marples, D., Kim, Y-H., Wang, W., Frøkiaer, J., & Nielsen, S. (2004). Changes in cellular composition of kidney collecting duct cells in rats with lithium-induced NDI. American Journal of Physiology: Cell Physiology, 286(4), C952-64. https://doi.org/10.1152/ajpcell.00266.2003

CBE

Christensen BM, Marples D, Kim Y-H, Wang W, Frøkiaer J, Nielsen S. 2004. Changes in cellular composition of kidney collecting duct cells in rats with lithium-induced NDI. American Journal of Physiology: Cell Physiology. 286(4):C952-64. https://doi.org/10.1152/ajpcell.00266.2003

MLA

Vancouver

Christensen BM, Marples D, Kim Y-H, Wang W, Frøkiaer J, Nielsen S. Changes in cellular composition of kidney collecting duct cells in rats with lithium-induced NDI. American Journal of Physiology: Cell Physiology. 2004;286(4):C952-64. https://doi.org/10.1152/ajpcell.00266.2003

Author

Christensen, Birgitte Mønster ; Marples, David ; Kim, Young-Hee ; Wang, Weidong ; Frøkiaer, Jørgen ; Nielsen, Søren. / Changes in cellular composition of kidney collecting duct cells in rats with lithium-induced NDI. In: American Journal of Physiology: Cell Physiology. 2004 ; Vol. 286, No. 4. pp. C952-64.

Bibtex

@article{11ae3730cc4611dd9710000ea68e967b,
title = "Changes in cellular composition of kidney collecting duct cells in rats with lithium-induced NDI",
abstract = "Lithium treatment for 4 wk caused severe polyuria, dramatic downregulation in aquaporin-2 (AQP-2) expression, and marked decrease in AQP-2 immunoreactivity with the appearance of a large number of cells without AQP-2 labeling in the collecting ducts after lithium treatment. Surprisingly, this was not all due to an increase in AQP-2-negative principal cells, because double immunolabeling revealed that the majority of the AQP-2-negative cells displayed [H(+)]ATPase labeling, which identified them as intercalated cells. Moreover, multiple [H(+)]ATPase-labeled cells were adjacent, which was never seen in control rats. Quantitation confirmed a significant decrease in the fraction of collecting duct cells that exhibited detectable AQP-2 labeling compared with control rats: in cortical collecting ducts, 40 +/- 3.4 vs. 62 +/- 1.8% of controls (P < 0.05; n = 4) and in inner medullary collecting ducts, 58 +/- 1.6 vs. 81 +/- 1.3% of controls (P < 0.05; n = 4). In parallel, a significant increase in the fraction of intercalated ([H(+)]ATPase-positive) cells was shown. Urine output, whole kidney AQP-2 expression, cellular organization, and the fractions of principal and intercalated cells in cortex and inner medulla returned to control levels after 4 wk on a lithium-free diet following 4 wk on a lithium-containing diet. In conclusion, lithium treatment not only decreased AQP-2 expression, but dramatically and reversibly reduced the fraction of principal cells and altered the cellular organization in collecting ducts. These effects are likely to be important in lithium-induced nephrogenic diabetes insipidus.",
keywords = "Animals, Antibodies, Aquaporin 2, Aquaporin 4, Aquaporin 6, Aquaporins, Carrier Proteins, Diabetes Insipidus, Nephrogenic, Down-Regulation, Kidney Tubules, Collecting, Lithium, Membrane Transport Proteins, Microscopy, Immunoelectron, Proton-Translocating ATPases, Rats, Rats, Wistar, Recovery of Function, Urine",
author = "Christensen, {Birgitte M{\o}nster} and David Marples and Young-Hee Kim and Weidong Wang and J{\o}rgen Fr{\o}kiaer and S{\o}ren Nielsen",
year = "2004",
doi = "10.1152/ajpcell.00266.2003",
language = "English",
volume = "286",
pages = "C952--64",
journal = "American Journal of Physiology: Cell Physiology",
issn = "0363-6143",
publisher = "American Physiological Society",
number = "4",

}

RIS

TY - JOUR

T1 - Changes in cellular composition of kidney collecting duct cells in rats with lithium-induced NDI

AU - Christensen, Birgitte Mønster

AU - Marples, David

AU - Kim, Young-Hee

AU - Wang, Weidong

AU - Frøkiaer, Jørgen

AU - Nielsen, Søren

PY - 2004

Y1 - 2004

N2 - Lithium treatment for 4 wk caused severe polyuria, dramatic downregulation in aquaporin-2 (AQP-2) expression, and marked decrease in AQP-2 immunoreactivity with the appearance of a large number of cells without AQP-2 labeling in the collecting ducts after lithium treatment. Surprisingly, this was not all due to an increase in AQP-2-negative principal cells, because double immunolabeling revealed that the majority of the AQP-2-negative cells displayed [H(+)]ATPase labeling, which identified them as intercalated cells. Moreover, multiple [H(+)]ATPase-labeled cells were adjacent, which was never seen in control rats. Quantitation confirmed a significant decrease in the fraction of collecting duct cells that exhibited detectable AQP-2 labeling compared with control rats: in cortical collecting ducts, 40 +/- 3.4 vs. 62 +/- 1.8% of controls (P < 0.05; n = 4) and in inner medullary collecting ducts, 58 +/- 1.6 vs. 81 +/- 1.3% of controls (P < 0.05; n = 4). In parallel, a significant increase in the fraction of intercalated ([H(+)]ATPase-positive) cells was shown. Urine output, whole kidney AQP-2 expression, cellular organization, and the fractions of principal and intercalated cells in cortex and inner medulla returned to control levels after 4 wk on a lithium-free diet following 4 wk on a lithium-containing diet. In conclusion, lithium treatment not only decreased AQP-2 expression, but dramatically and reversibly reduced the fraction of principal cells and altered the cellular organization in collecting ducts. These effects are likely to be important in lithium-induced nephrogenic diabetes insipidus.

AB - Lithium treatment for 4 wk caused severe polyuria, dramatic downregulation in aquaporin-2 (AQP-2) expression, and marked decrease in AQP-2 immunoreactivity with the appearance of a large number of cells without AQP-2 labeling in the collecting ducts after lithium treatment. Surprisingly, this was not all due to an increase in AQP-2-negative principal cells, because double immunolabeling revealed that the majority of the AQP-2-negative cells displayed [H(+)]ATPase labeling, which identified them as intercalated cells. Moreover, multiple [H(+)]ATPase-labeled cells were adjacent, which was never seen in control rats. Quantitation confirmed a significant decrease in the fraction of collecting duct cells that exhibited detectable AQP-2 labeling compared with control rats: in cortical collecting ducts, 40 +/- 3.4 vs. 62 +/- 1.8% of controls (P < 0.05; n = 4) and in inner medullary collecting ducts, 58 +/- 1.6 vs. 81 +/- 1.3% of controls (P < 0.05; n = 4). In parallel, a significant increase in the fraction of intercalated ([H(+)]ATPase-positive) cells was shown. Urine output, whole kidney AQP-2 expression, cellular organization, and the fractions of principal and intercalated cells in cortex and inner medulla returned to control levels after 4 wk on a lithium-free diet following 4 wk on a lithium-containing diet. In conclusion, lithium treatment not only decreased AQP-2 expression, but dramatically and reversibly reduced the fraction of principal cells and altered the cellular organization in collecting ducts. These effects are likely to be important in lithium-induced nephrogenic diabetes insipidus.

KW - Animals

KW - Antibodies

KW - Aquaporin 2

KW - Aquaporin 4

KW - Aquaporin 6

KW - Aquaporins

KW - Carrier Proteins

KW - Diabetes Insipidus, Nephrogenic

KW - Down-Regulation

KW - Kidney Tubules, Collecting

KW - Lithium

KW - Membrane Transport Proteins

KW - Microscopy, Immunoelectron

KW - Proton-Translocating ATPases

KW - Rats

KW - Rats, Wistar

KW - Recovery of Function

KW - Urine

U2 - 10.1152/ajpcell.00266.2003

DO - 10.1152/ajpcell.00266.2003

M3 - Journal article

C2 - 14613889

VL - 286

SP - C952-64

JO - American Journal of Physiology: Cell Physiology

JF - American Journal of Physiology: Cell Physiology

SN - 0363-6143

IS - 4

ER -