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Jørgen Frøkiær

Age-dependent renal expression of acid-base transporters in neonatal ureter obstruction

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Age-dependent renal expression of acid-base transporters in neonatal ureter obstruction. / Wang, Guixian; Topçu, Sükrü Oguzkan; Ring, Troels; Wen, Jianguo; Djurhuus, Jens Christian; Kwon, Tae-Hwan; Nielsen, Søren; Frøkiaer, Jørgen.

In: Pediatric Nephrology, Vol. 24, No. 8, 2009, p. 1487-500.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Wang, G, Topçu, SO, Ring, T, Wen, J, Djurhuus, JC, Kwon, T-H, Nielsen, S & Frøkiaer, J 2009, 'Age-dependent renal expression of acid-base transporters in neonatal ureter obstruction', Pediatric Nephrology, vol. 24, no. 8, pp. 1487-500. https://doi.org/10.1007/s00467-009-1193-y

APA

Wang, G., Topçu, S. O., Ring, T., Wen, J., Djurhuus, J. C., Kwon, T-H., Nielsen, S., & Frøkiaer, J. (2009). Age-dependent renal expression of acid-base transporters in neonatal ureter obstruction. Pediatric Nephrology, 24(8), 1487-500. https://doi.org/10.1007/s00467-009-1193-y

CBE

MLA

Vancouver

Wang G, Topçu SO, Ring T, Wen J, Djurhuus JC, Kwon T-H et al. Age-dependent renal expression of acid-base transporters in neonatal ureter obstruction. Pediatric Nephrology. 2009;24(8):1487-500. https://doi.org/10.1007/s00467-009-1193-y

Author

Wang, Guixian ; Topçu, Sükrü Oguzkan ; Ring, Troels ; Wen, Jianguo ; Djurhuus, Jens Christian ; Kwon, Tae-Hwan ; Nielsen, Søren ; Frøkiaer, Jørgen. / Age-dependent renal expression of acid-base transporters in neonatal ureter obstruction. In: Pediatric Nephrology. 2009 ; Vol. 24, No. 8. pp. 1487-500.

Bibtex

@article{fcbd96105feb11deb2cc000ea68e967b,
title = "Age-dependent renal expression of acid-base transporters in neonatal ureter obstruction",
abstract = "Congenital obstructive nephropathy accounts for a major proportion of renal insufficiency in infancy and childhood. In an earlier investigation we demonstrated that bilateral complete ureteral obstruction (BUO) in rats is associated with inadequate urinary acidification [Am J Physiol Renal Physiol. 295(2):F497-506, 2008]. The aim of the study reported here was to determine whether this defect is also associated with unilateral ureteral obstruction (UUO), which is clinically more common than BUO. The time-course of the changes in protein expression levels of major renal acid-base transporters was examined at 7 and 14 weeks in rats with neonatally induced partial unilateral ureteral obstruction (PUUO), which was performed within the first 48 h of life. We observed that protein expression of the renal acid-base transporters NHE3, NBC1, NBCn1, pendrin and Na(+)-K(+)-ATPase was increased in both obstructed and non-obstructed kidneys 7 weeks after the induction of neonatal PUUO. This was confirmed by immunocytochemistry. In contrast, 14 weeks after the induction of PUUO, there was a significant downregulation of the renal acid-base transporters NBC1, NBCn1 and Na(+)-K(+)-ATPase in the obstructed kidneys. These time/age-dependent changes in protein expression were associated with parallel changes in renal function resulting in urine acidification in response to exogenous acid loading. In conclusion, these results show that downregulation of protein expression is a time/age-dependent response to PUUO, which could contribute to the decreased net acid excretion and development of metabolic acidosis in neonatal rats with PUUO.",
author = "Guixian Wang and Top{\c c}u, {S{\"u}kr{\"u} Oguzkan} and Troels Ring and Jianguo Wen and Djurhuus, {Jens Christian} and Tae-Hwan Kwon and S{\o}ren Nielsen and J{\o}rgen Fr{\o}kiaer",
year = "2009",
doi = "10.1007/s00467-009-1193-y",
language = "English",
volume = "24",
pages = "1487--500",
journal = "Pediatric Nephrology",
issn = "0931-041X",
publisher = "Springer",
number = "8",

}

RIS

TY - JOUR

T1 - Age-dependent renal expression of acid-base transporters in neonatal ureter obstruction

AU - Wang, Guixian

AU - Topçu, Sükrü Oguzkan

AU - Ring, Troels

AU - Wen, Jianguo

AU - Djurhuus, Jens Christian

AU - Kwon, Tae-Hwan

AU - Nielsen, Søren

AU - Frøkiaer, Jørgen

PY - 2009

Y1 - 2009

N2 - Congenital obstructive nephropathy accounts for a major proportion of renal insufficiency in infancy and childhood. In an earlier investigation we demonstrated that bilateral complete ureteral obstruction (BUO) in rats is associated with inadequate urinary acidification [Am J Physiol Renal Physiol. 295(2):F497-506, 2008]. The aim of the study reported here was to determine whether this defect is also associated with unilateral ureteral obstruction (UUO), which is clinically more common than BUO. The time-course of the changes in protein expression levels of major renal acid-base transporters was examined at 7 and 14 weeks in rats with neonatally induced partial unilateral ureteral obstruction (PUUO), which was performed within the first 48 h of life. We observed that protein expression of the renal acid-base transporters NHE3, NBC1, NBCn1, pendrin and Na(+)-K(+)-ATPase was increased in both obstructed and non-obstructed kidneys 7 weeks after the induction of neonatal PUUO. This was confirmed by immunocytochemistry. In contrast, 14 weeks after the induction of PUUO, there was a significant downregulation of the renal acid-base transporters NBC1, NBCn1 and Na(+)-K(+)-ATPase in the obstructed kidneys. These time/age-dependent changes in protein expression were associated with parallel changes in renal function resulting in urine acidification in response to exogenous acid loading. In conclusion, these results show that downregulation of protein expression is a time/age-dependent response to PUUO, which could contribute to the decreased net acid excretion and development of metabolic acidosis in neonatal rats with PUUO.

AB - Congenital obstructive nephropathy accounts for a major proportion of renal insufficiency in infancy and childhood. In an earlier investigation we demonstrated that bilateral complete ureteral obstruction (BUO) in rats is associated with inadequate urinary acidification [Am J Physiol Renal Physiol. 295(2):F497-506, 2008]. The aim of the study reported here was to determine whether this defect is also associated with unilateral ureteral obstruction (UUO), which is clinically more common than BUO. The time-course of the changes in protein expression levels of major renal acid-base transporters was examined at 7 and 14 weeks in rats with neonatally induced partial unilateral ureteral obstruction (PUUO), which was performed within the first 48 h of life. We observed that protein expression of the renal acid-base transporters NHE3, NBC1, NBCn1, pendrin and Na(+)-K(+)-ATPase was increased in both obstructed and non-obstructed kidneys 7 weeks after the induction of neonatal PUUO. This was confirmed by immunocytochemistry. In contrast, 14 weeks after the induction of PUUO, there was a significant downregulation of the renal acid-base transporters NBC1, NBCn1 and Na(+)-K(+)-ATPase in the obstructed kidneys. These time/age-dependent changes in protein expression were associated with parallel changes in renal function resulting in urine acidification in response to exogenous acid loading. In conclusion, these results show that downregulation of protein expression is a time/age-dependent response to PUUO, which could contribute to the decreased net acid excretion and development of metabolic acidosis in neonatal rats with PUUO.

U2 - 10.1007/s00467-009-1193-y

DO - 10.1007/s00467-009-1193-y

M3 - Journal article

C2 - 19495807

VL - 24

SP - 1487

EP - 1500

JO - Pediatric Nephrology

JF - Pediatric Nephrology

SN - 0931-041X

IS - 8

ER -