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Jette F Young

NMR-Based Metabonomic Investigation of Heat Stress in Myotubes Reveals a Time-Dependent Change in the Metabolites

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  • Nutrition and Production Physiology
  • Dairy, Meat and Egg Quality and Technology
  • Plant Food Science
  • Department of Food Science
  • Department of Animal Health and Bioscience
  • Research Unit for Molecular Medicine
NMR-based metabonomics was applied to elucidate the time-dependent stress responses in mouse myotubes after heat exposure of either 42 or 45 degrees C for 1 h. Principal component analysis (PCA) revealed that the gradual time-dependent changes in metabolites contributing to the clustering and separation of the control samples from the different time points after heat stress primarily are in the metabolites glucose, leucine, lysine, phenylalanine, creatine, glutamine, and acetate. In addition, PC scores revealed a maximum change in metabolite composition 4 h after the stress exposure; thereafter, samples returned toward control samples, however, without reaching the control samples even 10 h after stress. The results also indicate that the myotubes efficiently regulate the pH level by release of lactate to the culture medium at a heat stress level of 42 degrees C, which is a temperature level reached in muscles of pigs during exposure to slaughter stress.
Original languageEnglish
JournalJournal of Agricultural and Food Chemistry
Pages (from-to)6376-6386
Number of pages11
Publication statusPublished - 2010

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