Jens Randel Nyengaard

Zebrafish Whole-Mount In Situ Hybridization Followed by Sectioning

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In situ hybridization is a powerful technique used for locating specific nucleic acid targets within morphologically preserved tissues and cell preparations. A labeled RNA or DNA probe hybridizes to its complementary mRNA or DNA sequence within a sample. Here, we describe RNA in situ hybridization protocol for whole-mount zebrafish embryos.

Original languageEnglish
JournalMethods in molecular biology (Clifton, N.J.)
Pages (from-to)353-63
Number of pages11
Publication statusPublished - 2016

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